Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain
The intracellular actions of the antidepressant, venlafaxine, were studied in C6-gliomas using a phosphoproteomics approach. Long-term pre-treatment of C6-gliomas with venlafaxine followed by an acute challenge with isoproterenol (a β-adrenoceptor agonist), resulted in increased p90Rsk phosphorylati...
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| Veröffentlicht in: | Neuroscience letters 2004-11, Vol.372 (1), p.99-103 |
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| creator | Khawaja, Xavier Z. Storm, Stephen Liang, Jin-Jun |
| description | The intracellular actions of the antidepressant, venlafaxine, were studied in C6-gliomas using a phosphoproteomics approach. Long-term pre-treatment of C6-gliomas with venlafaxine followed by an acute challenge with isoproterenol (a β-adrenoceptor agonist), resulted in increased p90Rsk phosphorylation (three-fold) versus control levels (isoproterenol alone). The effect of venlafaxine pre-treatment on p90Rsk activity was dose-dependent (EC
50
=
3.75
nM) in C6 gliomas. In rat brain sections, intense immunoreactive phospho-p90Rsk labeling was observed for both neurons and glia, especially in cortical layers II/III and hippocampal formations. In vivo studies demonstrated an intense but similar distribution pattern of phospho-p90Rsk staining after chronic venlafaxine dosing of rats compared to naives and no region-specific drug effect was observed in vivo. In conclusion, our findings suggest that some of the centrally-mediated benefits of venlafaxine in depression may be due to its intracellular properties especially on the neuro-glial circuitry and MAPK/p90Rsk-dependent pathways at an early stage. |
| doi_str_mv | 10.1016/j.neulet.2004.09.029 |
| format | Article |
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50
=
3.75
nM) in C6 gliomas. In rat brain sections, intense immunoreactive phospho-p90Rsk labeling was observed for both neurons and glia, especially in cortical layers II/III and hippocampal formations. In vivo studies demonstrated an intense but similar distribution pattern of phospho-p90Rsk staining after chronic venlafaxine dosing of rats compared to naives and no region-specific drug effect was observed in vivo. In conclusion, our findings suggest that some of the centrally-mediated benefits of venlafaxine in depression may be due to its intracellular properties especially on the neuro-glial circuitry and MAPK/p90Rsk-dependent pathways at an early stage.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2004.09.029</identifier><identifier>PMID: 15531096</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Brain - drug effects ; Brain - metabolism ; C6-glioma ; Cell Line, Tumor ; Cyclohexanols - pharmacology ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Glioma - metabolism ; Male ; p90Rsk ; Phosphoproteomics ; Rat brain ; Rats ; Rats, Sprague-Dawley ; Ribosomal Protein S6 Kinases, 90-kDa - metabolism ; Signal transduction ; Venlafaxine Hydrochloride ; Vertebrates: nervous system and sense organs ; β-Adrenergic receptors</subject><ispartof>Neuroscience letters, 2004-11, Vol.372 (1), p.99-103</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-519a6c0bd3ee27d437def0ff0221802b26679fc3e1efb1317f2004c7fb97035b3</citedby><cites>FETCH-LOGICAL-c388t-519a6c0bd3ee27d437def0ff0221802b26679fc3e1efb1317f2004c7fb97035b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2004.09.029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16258903$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15531096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khawaja, Xavier Z.</creatorcontrib><creatorcontrib>Storm, Stephen</creatorcontrib><creatorcontrib>Liang, Jin-Jun</creatorcontrib><title>Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The intracellular actions of the antidepressant, venlafaxine, were studied in C6-gliomas using a phosphoproteomics approach. Long-term pre-treatment of C6-gliomas with venlafaxine followed by an acute challenge with isoproterenol (a β-adrenoceptor agonist), resulted in increased p90Rsk phosphorylation (three-fold) versus control levels (isoproterenol alone). The effect of venlafaxine pre-treatment on p90Rsk activity was dose-dependent (EC
50
=
3.75
nM) in C6 gliomas. In rat brain sections, intense immunoreactive phospho-p90Rsk labeling was observed for both neurons and glia, especially in cortical layers II/III and hippocampal formations. In vivo studies demonstrated an intense but similar distribution pattern of phospho-p90Rsk staining after chronic venlafaxine dosing of rats compared to naives and no region-specific drug effect was observed in vivo. In conclusion, our findings suggest that some of the centrally-mediated benefits of venlafaxine in depression may be due to its intracellular properties especially on the neuro-glial circuitry and MAPK/p90Rsk-dependent pathways at an early stage.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>C6-glioma</subject><subject>Cell Line, Tumor</subject><subject>Cyclohexanols - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glioma - metabolism</subject><subject>Male</subject><subject>p90Rsk</subject><subject>Phosphoproteomics</subject><subject>Rat brain</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Ribosomal Protein S6 Kinases, 90-kDa - metabolism</subject><subject>Signal transduction</subject><subject>Venlafaxine Hydrochloride</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>β-Adrenergic receptors</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A5Fc9NY6SdqmuQiyrB-wIIieQ5pOJGs3XZPuov_eLrvgzdNcnvedmYeQSwY5A1bdLvKA6w6HnAMUOagcuDogE1ZLnkkl-SGZgIAiE6qAE3Ka0gIASlYWx-SElaVgoKoJmc2cQzsk2ju6wdAZZ759QNoHulLwmj6psYPf-OGH-kCjGei0yj463y9Noia0tInGh3Ny5EyX8GI_z8j7w-xt-pTNXx6fp_fzzIq6HrKSKVNZaFqByGVbCNmiA-eAc1YDb3hVSeWsQIauYYJJt_3NStcoCaJsxBm52fWuYv-1xjTopU8Wu84E7NdJVxJKxQoYwWIH2tinFNHpVfRLE380A73Vpxd6p09vV2hQetQ3xq72_etmie1faO9rBK73gEnWdC6aYH364ype1grEyN3tOBxtbDxGnazHYLH1cdSt297_f8kvVM-OTA</recordid><startdate>20041130</startdate><enddate>20041130</enddate><creator>Khawaja, Xavier Z.</creator><creator>Storm, Stephen</creator><creator>Liang, Jin-Jun</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041130</creationdate><title>Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain</title><author>Khawaja, Xavier Z. ; Storm, Stephen ; Liang, Jin-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-519a6c0bd3ee27d437def0ff0221802b26679fc3e1efb1317f2004c7fb97035b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>C6-glioma</topic><topic>Cell Line, Tumor</topic><topic>Cyclohexanols - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glioma - metabolism</topic><topic>Male</topic><topic>p90Rsk</topic><topic>Phosphoproteomics</topic><topic>Rat brain</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Ribosomal Protein S6 Kinases, 90-kDa - metabolism</topic><topic>Signal transduction</topic><topic>Venlafaxine Hydrochloride</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>β-Adrenergic receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khawaja, Xavier Z.</creatorcontrib><creatorcontrib>Storm, Stephen</creatorcontrib><creatorcontrib>Liang, Jin-Jun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khawaja, Xavier Z.</au><au>Storm, Stephen</au><au>Liang, Jin-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2004-11-30</date><risdate>2004</risdate><volume>372</volume><issue>1</issue><spage>99</spage><epage>103</epage><pages>99-103</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The intracellular actions of the antidepressant, venlafaxine, were studied in C6-gliomas using a phosphoproteomics approach. Long-term pre-treatment of C6-gliomas with venlafaxine followed by an acute challenge with isoproterenol (a β-adrenoceptor agonist), resulted in increased p90Rsk phosphorylation (three-fold) versus control levels (isoproterenol alone). The effect of venlafaxine pre-treatment on p90Rsk activity was dose-dependent (EC
50
=
3.75
nM) in C6 gliomas. In rat brain sections, intense immunoreactive phospho-p90Rsk labeling was observed for both neurons and glia, especially in cortical layers II/III and hippocampal formations. In vivo studies demonstrated an intense but similar distribution pattern of phospho-p90Rsk staining after chronic venlafaxine dosing of rats compared to naives and no region-specific drug effect was observed in vivo. In conclusion, our findings suggest that some of the centrally-mediated benefits of venlafaxine in depression may be due to its intracellular properties especially on the neuro-glial circuitry and MAPK/p90Rsk-dependent pathways at an early stage.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15531096</pmid><doi>10.1016/j.neulet.2004.09.029</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neuroscience letters, 2004-11, Vol.372 (1), p.99-103 |
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| subjects | Animals Biological and medical sciences Brain - drug effects Brain - metabolism C6-glioma Cell Line, Tumor Cyclohexanols - pharmacology Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Glioma - metabolism Male p90Rsk Phosphoproteomics Rat brain Rats Rats, Sprague-Dawley Ribosomal Protein S6 Kinases, 90-kDa - metabolism Signal transduction Venlafaxine Hydrochloride Vertebrates: nervous system and sense organs β-Adrenergic receptors |
| title | Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain |
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