Activation of extracellular signal-regulated kinases in the sciatic nerves of rats with experimental autoimmune neuritis
To investigate whether the phosphorylation of extracellular signal-regulated kinase (ERK) is involved in autoimmune injury of the peripheral nervous system (PNS), the expression of phosphorylated ERK (p-ERK) was analyzed in experimental autoimmune neuritis (EAN) in rats. Western blot analysis showed...
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| Veröffentlicht in: | Neuroscience letters 2004-11, Vol.372 (1), p.57-61 |
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| creator | Ahn, Meejung Moon, Changjong Lee, Yongduk Koh, Chang Sung Kohyama, Kuniko Tanuma, Naoyuki Matsumoto, Yoh Kim, Hyung-Min Kim, Seung-Ryul Shin, Taekyun |
| description | To investigate whether the phosphorylation of extracellular signal-regulated kinase (ERK) is involved in autoimmune injury of the peripheral nervous system (PNS), the expression of phosphorylated ERK (p-ERK) was analyzed in experimental autoimmune neuritis (EAN) in rats. Western blot analysis showed that the level of p-ERK was increased significantly in the sciatic nerves of rats on days 14 (
p
<
0.05) and 24 (
p
<
0.01) post-immunization, compared with controls, and its reaction declined at day 30 post-immunization. Immunohistochemistry showed that p-ERK protein was weakly expressed in Schwann cells and vascular endothelial cells in the sciatic nerves of CFA-immunized control rats. In EAN-affected sciatic nerves, p-ERK immunoreactivity was found mainly in ED1-positive macrophages on days 14 and 24 post-immunization. Moreover, on days 24 and 30 post-immunization, p-ERK immunoreactivity increased gradually in the Schwann cells of rat sciatic nerves with EAN. Based on these results, we postulated that the phosphorylation of ERK has an important role in the differentiation and survival of cells, including inflammatory cells and Schwann cells, in the rat sciatic nerve in EAN. Specifically, the activation of ERK in the recovery phase of EAN paralysis seems to be related in the survival of Schwann cells. |
| doi_str_mv | 10.1016/j.neulet.2004.09.016 |
| format | Article |
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p
<
0.05) and 24 (
p
<
0.01) post-immunization, compared with controls, and its reaction declined at day 30 post-immunization. Immunohistochemistry showed that p-ERK protein was weakly expressed in Schwann cells and vascular endothelial cells in the sciatic nerves of CFA-immunized control rats. In EAN-affected sciatic nerves, p-ERK immunoreactivity was found mainly in ED1-positive macrophages on days 14 and 24 post-immunization. Moreover, on days 24 and 30 post-immunization, p-ERK immunoreactivity increased gradually in the Schwann cells of rat sciatic nerves with EAN. Based on these results, we postulated that the phosphorylation of ERK has an important role in the differentiation and survival of cells, including inflammatory cells and Schwann cells, in the rat sciatic nerve in EAN. Specifically, the activation of ERK in the recovery phase of EAN paralysis seems to be related in the survival of Schwann cells.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2004.09.016</identifier><identifier>PMID: 15531088</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Demyelination ; Enzyme Activation - physiology ; Experimental autoimmune neuritis ; Extracellular signal-regulated kinase ; Extracellular Signal-Regulated MAP Kinases - immunology ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Medical sciences ; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis ; Neuritis, Autoimmune, Experimental - enzymology ; Neuritis, Autoimmune, Experimental - immunology ; Neurology ; Rats ; Rats, Inbred Lew ; Schwann cell ; Sciatic nerve ; Sciatic Nerve - enzymology ; Sciatic Nerve - immunology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2004-11, Vol.372 (1), p.57-61</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-76b5ab78ce6897624eb909c92efe379a822070d158c4d5097836ca2666faea143</citedby><cites>FETCH-LOGICAL-c388t-76b5ab78ce6897624eb909c92efe379a822070d158c4d5097836ca2666faea143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2004.09.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16258895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15531088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahn, Meejung</creatorcontrib><creatorcontrib>Moon, Changjong</creatorcontrib><creatorcontrib>Lee, Yongduk</creatorcontrib><creatorcontrib>Koh, Chang Sung</creatorcontrib><creatorcontrib>Kohyama, Kuniko</creatorcontrib><creatorcontrib>Tanuma, Naoyuki</creatorcontrib><creatorcontrib>Matsumoto, Yoh</creatorcontrib><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Kim, Seung-Ryul</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><title>Activation of extracellular signal-regulated kinases in the sciatic nerves of rats with experimental autoimmune neuritis</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>To investigate whether the phosphorylation of extracellular signal-regulated kinase (ERK) is involved in autoimmune injury of the peripheral nervous system (PNS), the expression of phosphorylated ERK (p-ERK) was analyzed in experimental autoimmune neuritis (EAN) in rats. Western blot analysis showed that the level of p-ERK was increased significantly in the sciatic nerves of rats on days 14 (
p
<
0.05) and 24 (
p
<
0.01) post-immunization, compared with controls, and its reaction declined at day 30 post-immunization. Immunohistochemistry showed that p-ERK protein was weakly expressed in Schwann cells and vascular endothelial cells in the sciatic nerves of CFA-immunized control rats. In EAN-affected sciatic nerves, p-ERK immunoreactivity was found mainly in ED1-positive macrophages on days 14 and 24 post-immunization. Moreover, on days 24 and 30 post-immunization, p-ERK immunoreactivity increased gradually in the Schwann cells of rat sciatic nerves with EAN. Based on these results, we postulated that the phosphorylation of ERK has an important role in the differentiation and survival of cells, including inflammatory cells and Schwann cells, in the rat sciatic nerve in EAN. Specifically, the activation of ERK in the recovery phase of EAN paralysis seems to be related in the survival of Schwann cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Demyelination</subject><subject>Enzyme Activation - physiology</subject><subject>Experimental autoimmune neuritis</subject><subject>Extracellular signal-regulated kinase</subject><subject>Extracellular Signal-Regulated MAP Kinases - immunology</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Medical sciences</subject><subject>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</subject><subject>Neuritis, Autoimmune, Experimental - enzymology</subject><subject>Neuritis, Autoimmune, Experimental - immunology</subject><subject>Neurology</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Schwann cell</subject><subject>Sciatic nerve</subject><subject>Sciatic Nerve - enzymology</subject><subject>Sciatic Nerve - immunology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EotvCP0DIF7gl2E78dUGqKqBIlbjA2Zp1Jq2XxFlsZ1v-PV7tSr1xsjx63lczDyHvOGs54-rTro24TlhawVjfMtvW4Quy4UaLRlstXpIN61jfdLZnF-Qy5x1jTHLZvyYXXMqOM2M25Onal3CAEpZIl5HiU0ngcZrWCRLN4T7C1CS8r9-CA_0dImTMNERaHpBmH2rS04jpUKc1n6Bk-hjKQ23aYwozxgIThbUsYZ7XiJVdUyghvyGvRpgyvj2_V-TX1y8_b26bux_fvt9c3zW-M6Y0Wm0lbLXxqIzVSvS4tcx6K3DETlswQjDNBi6N7wfJrDad8iCUUiMg8L67Ih9Pvfu0_FkxFzeHfLwQIi5rdkozaTrNK9ifQJ-WnBOObl_3h_TXceaOxt3OnYy7o3HHrKvDGnt_7l-3Mw7PobPiCnw4A5A9TGOC6EN-5pSQxlhZuc8nDquNQ8Dkql6MHoeQ0Bc3LOH_m_wDDVWjiw</recordid><startdate>20041130</startdate><enddate>20041130</enddate><creator>Ahn, Meejung</creator><creator>Moon, Changjong</creator><creator>Lee, Yongduk</creator><creator>Koh, Chang Sung</creator><creator>Kohyama, Kuniko</creator><creator>Tanuma, Naoyuki</creator><creator>Matsumoto, Yoh</creator><creator>Kim, Hyung-Min</creator><creator>Kim, Seung-Ryul</creator><creator>Shin, Taekyun</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041130</creationdate><title>Activation of extracellular signal-regulated kinases in the sciatic nerves of rats with experimental autoimmune neuritis</title><author>Ahn, Meejung ; Moon, Changjong ; Lee, Yongduk ; Koh, Chang Sung ; Kohyama, Kuniko ; Tanuma, Naoyuki ; Matsumoto, Yoh ; Kim, Hyung-Min ; Kim, Seung-Ryul ; Shin, Taekyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-76b5ab78ce6897624eb909c92efe379a822070d158c4d5097836ca2666faea143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Demyelination</topic><topic>Enzyme Activation - physiology</topic><topic>Experimental autoimmune neuritis</topic><topic>Extracellular signal-regulated kinase</topic><topic>Extracellular Signal-Regulated MAP Kinases - immunology</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Medical sciences</topic><topic>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</topic><topic>Neuritis, Autoimmune, Experimental - enzymology</topic><topic>Neuritis, Autoimmune, Experimental - immunology</topic><topic>Neurology</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Schwann cell</topic><topic>Sciatic nerve</topic><topic>Sciatic Nerve - enzymology</topic><topic>Sciatic Nerve - immunology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahn, Meejung</creatorcontrib><creatorcontrib>Moon, Changjong</creatorcontrib><creatorcontrib>Lee, Yongduk</creatorcontrib><creatorcontrib>Koh, Chang Sung</creatorcontrib><creatorcontrib>Kohyama, Kuniko</creatorcontrib><creatorcontrib>Tanuma, Naoyuki</creatorcontrib><creatorcontrib>Matsumoto, Yoh</creatorcontrib><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Kim, Seung-Ryul</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahn, Meejung</au><au>Moon, Changjong</au><au>Lee, Yongduk</au><au>Koh, Chang Sung</au><au>Kohyama, Kuniko</au><au>Tanuma, Naoyuki</au><au>Matsumoto, Yoh</au><au>Kim, Hyung-Min</au><au>Kim, Seung-Ryul</au><au>Shin, Taekyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of extracellular signal-regulated kinases in the sciatic nerves of rats with experimental autoimmune neuritis</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2004-11-30</date><risdate>2004</risdate><volume>372</volume><issue>1</issue><spage>57</spage><epage>61</epage><pages>57-61</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>To investigate whether the phosphorylation of extracellular signal-regulated kinase (ERK) is involved in autoimmune injury of the peripheral nervous system (PNS), the expression of phosphorylated ERK (p-ERK) was analyzed in experimental autoimmune neuritis (EAN) in rats. Western blot analysis showed that the level of p-ERK was increased significantly in the sciatic nerves of rats on days 14 (
p
<
0.05) and 24 (
p
<
0.01) post-immunization, compared with controls, and its reaction declined at day 30 post-immunization. Immunohistochemistry showed that p-ERK protein was weakly expressed in Schwann cells and vascular endothelial cells in the sciatic nerves of CFA-immunized control rats. In EAN-affected sciatic nerves, p-ERK immunoreactivity was found mainly in ED1-positive macrophages on days 14 and 24 post-immunization. Moreover, on days 24 and 30 post-immunization, p-ERK immunoreactivity increased gradually in the Schwann cells of rat sciatic nerves with EAN. Based on these results, we postulated that the phosphorylation of ERK has an important role in the differentiation and survival of cells, including inflammatory cells and Schwann cells, in the rat sciatic nerve in EAN. Specifically, the activation of ERK in the recovery phase of EAN paralysis seems to be related in the survival of Schwann cells.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15531088</pmid><doi>10.1016/j.neulet.2004.09.016</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Demyelination Enzyme Activation - physiology Experimental autoimmune neuritis Extracellular signal-regulated kinase Extracellular Signal-Regulated MAP Kinases - immunology Extracellular Signal-Regulated MAP Kinases - metabolism Female Fundamental and applied biological sciences. Psychology Medical sciences Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Neuritis, Autoimmune, Experimental - enzymology Neuritis, Autoimmune, Experimental - immunology Neurology Rats Rats, Inbred Lew Schwann cell Sciatic nerve Sciatic Nerve - enzymology Sciatic Nerve - immunology Vertebrates: nervous system and sense organs |
| title | Activation of extracellular signal-regulated kinases in the sciatic nerves of rats with experimental autoimmune neuritis |
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