Recombinant viruses obtained from co-infection in vitro with a live vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus display different plaque phenotypes and loss of the transgene
Some poxviruses are very attractive as transgenic vaccine vectors for humans, domestic animals and wildlife. Poxviridae family members circulate in different ecosystems and parts of the world, providing a pool of possible recombination partners for released or escaped genetically modified poxviruses...
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| Veröffentlicht in: | Vaccine 2004-12, Vol.23 (4), p.499-506 |
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| creator | Hansen, Hilde Okeke, Malachy Ifeanyi Nilssen, Øivind Traavik, Terje |
| description | Some poxviruses are very attractive as transgenic vaccine vectors for humans, domestic animals and wildlife.
Poxviridae family members circulate in different ecosystems and parts of the world, providing a pool of possible recombination partners for released or escaped genetically modified poxviruses. We performed in vitro double infections with a vaccinia virus strain Ankara (MVA) vectored influenza vaccine and a cowpox virus isolate from Norway, isolated hybrids, and further analyzed three hybrid viruses with different plaque phenotypes. One of the hybrids was genetically unstable, and during adaptation to new host cells its MVA derived influenza gene was deleted at a high frequency. This is significant in a risk assessment context, since the transgene would be the only logical tag for monitoring unwanted spread and non-target effects of a vaccine virus. Putative recombination events involving genetically modified and naturally occurring viruses should be included in health and environmental risk assessments. |
| doi_str_mv | 10.1016/j.vaccine.2004.06.032 |
| format | Article |
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Poxviridae family members circulate in different ecosystems and parts of the world, providing a pool of possible recombination partners for released or escaped genetically modified poxviruses. We performed in vitro double infections with a vaccinia virus strain Ankara (MVA) vectored influenza vaccine and a cowpox virus isolate from Norway, isolated hybrids, and further analyzed three hybrid viruses with different plaque phenotypes. One of the hybrids was genetically unstable, and during adaptation to new host cells its MVA derived influenza gene was deleted at a high frequency. This is significant in a risk assessment context, since the transgene would be the only logical tag for monitoring unwanted spread and non-target effects of a vaccine virus. Putative recombination events involving genetically modified and naturally occurring viruses should be included in health and environmental risk assessments.</description><subject>Animals</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>Cowpox virus</subject><subject>Cowpox virus - genetics</subject><subject>Cowpox virus - growth & development</subject><subject>DNA, Viral - isolation & purification</subject><subject>Domestic animals</subject><subject>Environmental assessment</subject><subject>Environmental risk</subject><subject>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hansen, Hilde</au><au>Okeke, Malachy Ifeanyi</au><au>Nilssen, Øivind</au><au>Traavik, Terje</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant viruses obtained from co-infection in vitro with a live vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus display different plaque phenotypes and loss of the transgene</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2004-12-09</date><risdate>2004</risdate><volume>23</volume><issue>4</issue><spage>499</spage><epage>506</epage><pages>499-506</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Some poxviruses are very attractive as transgenic vaccine vectors for humans, domestic animals and wildlife.
Poxviridae family members circulate in different ecosystems and parts of the world, providing a pool of possible recombination partners for released or escaped genetically modified poxviruses. We performed in vitro double infections with a vaccinia virus strain Ankara (MVA) vectored influenza vaccine and a cowpox virus isolate from Norway, isolated hybrids, and further analyzed three hybrid viruses with different plaque phenotypes. One of the hybrids was genetically unstable, and during adaptation to new host cells its MVA derived influenza gene was deleted at a high frequency. This is significant in a risk assessment context, since the transgene would be the only logical tag for monitoring unwanted spread and non-target effects of a vaccine virus. Putative recombination events involving genetically modified and naturally occurring viruses should be included in health and environmental risk assessments.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15530698</pmid><doi>10.1016/j.vaccine.2004.06.032</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Applied microbiology Biological and medical sciences Blotting, Southern Cell Line Cercopithecus aethiops Cowpox virus Cowpox virus - genetics Cowpox virus - growth & development DNA, Viral - isolation & purification Domestic animals Environmental assessment Environmental risk Fundamental and applied biological sciences. Psychology Genes Genomes Hybrids Immunization Infections Influenza Influenza Vaccines - genetics Influenza virus Microbiology Miscellaneous MVA recombinant vaccines Organisms, Genetically Modified Orthopoxvirus Phenotype Polymerase Chain Reaction Poxvirus Proteins Reassortant Viruses - genetics Reassortant Viruses - physiology Recombination, Genetic Risk assessment Safety Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccinia virus Vaccinia virus - genetics Vaccinia virus - growth & development Vero Cells Viral Plaque Assay Viral Vaccines - genetics Virology Viruses Wildlife |
| title | Recombinant viruses obtained from co-infection in vitro with a live vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus display different plaque phenotypes and loss of the transgene |
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