Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor
This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis sp...
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| Veröffentlicht in: | Analyst (London) 2009-04, Vol.134 (4), p.738-742 |
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| container_title | Analyst (London) |
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| creator | WANG, Dian-Bing BI, Li-Jun ZHANG, Zhi-Ping CHEN, Yuan-Yuan YANG, Rui-Fu WEI, Hong-Ping ZHOU, Ya-Feng ZHANG, Xian-En |
| description | This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores. |
| doi_str_mv | 10.1039/b813038h |
| format | Article |
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The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/b813038h</identifier><identifier>PMID: 19305924</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical chemistry ; Antibodies, Monoclonal - chemistry ; Antigens, Bacterial - chemistry ; Bacillus ; Bacillus anthracis - physiology ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Methods. Procedures. Technologies ; Spores, Bacterial ; Surface Plasmon Resonance - methods ; Various methods and equipments</subject><ispartof>Analyst (London), 2009-04, Vol.134 (4), p.738-742</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-a57ae0318636a1edd3dccea69c2affe1b46134e9f8cef764254cf3101f3357ee3</citedby><cites>FETCH-LOGICAL-c459t-a57ae0318636a1edd3dccea69c2affe1b46134e9f8cef764254cf3101f3357ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2832,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21348868$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19305924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, Dian-Bing</creatorcontrib><creatorcontrib>BI, Li-Jun</creatorcontrib><creatorcontrib>ZHANG, Zhi-Ping</creatorcontrib><creatorcontrib>CHEN, Yuan-Yuan</creatorcontrib><creatorcontrib>YANG, Rui-Fu</creatorcontrib><creatorcontrib>WEI, Hong-Ping</creatorcontrib><creatorcontrib>ZHOU, Ya-Feng</creatorcontrib><creatorcontrib>ZHANG, Xian-En</creatorcontrib><title>Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.</description><subject>Analytical chemistry</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antigens, Bacterial - chemistry</subject><subject>Bacillus</subject><subject>Bacillus anthracis - physiology</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Methods. Procedures. 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The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>19305924</pmid><doi>10.1039/b813038h</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry Antibodies, Monoclonal - chemistry Antigens, Bacterial - chemistry Bacillus Bacillus anthracis - physiology Biological and medical sciences Biosensors Biotechnology Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Methods. Procedures. Technologies Spores, Bacterial Surface Plasmon Resonance - methods Various methods and equipments |
| title | Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor |
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