Immunochemical approach to the problem of differential determination of natural forms of abscisic acid
An original modification of the standard ELISA procedure for differential determination of different forms of abscisic acid (ABA) is proposed. It is shown that endogenous forms of ABA may be quantitatively determined in plant tissues subjected to minimal treatment, without purification of the hormon...
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| Veröffentlicht in: | Biochemistry (Moscow) 2004-10, Vol.69 (10), p.1099-1108 |
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| creator | Blintsov, A N Gussakovskaya, M A |
| description | An original modification of the standard ELISA procedure for differential determination of different forms of abscisic acid (ABA) is proposed. It is shown that endogenous forms of ABA may be quantitatively determined in plant tissues subjected to minimal treatment, without purification of the hormones and their chemical modification. The modification has been approved when analyzing changes in the content of different ABA forms in plant tissues differing in physiological activity. Quantitative differential determination of changes in the content of different ABA forms has been performed in ovaries of Triticum aestivum L. and Taraxacum officinale Web. in the period of activity of the ovule (from the moment of its activation to the beginning of division). It is shown that, despite the different types of reproduction in the species studied (amphimixis and apomixis), the time course of changes in the content of different forms of ABA in ovaries is similar, which is suggestive of a correlation between the activity of endogenous hormonal system and chronology of main events (e.g., the beginning of endospermogenesis) of the reproductive cycle. |
| doi_str_mv | 10.1023/B:BIRY.0000046883.07615.e5 |
| format | Article |
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It is shown that endogenous forms of ABA may be quantitatively determined in plant tissues subjected to minimal treatment, without purification of the hormones and their chemical modification. The modification has been approved when analyzing changes in the content of different ABA forms in plant tissues differing in physiological activity. Quantitative differential determination of changes in the content of different ABA forms has been performed in ovaries of Triticum aestivum L. and Taraxacum officinale Web. in the period of activity of the ovule (from the moment of its activation to the beginning of division). It is shown that, despite the different types of reproduction in the species studied (amphimixis and apomixis), the time course of changes in the content of different forms of ABA in ovaries is similar, which is suggestive of a correlation between the activity of endogenous hormonal system and chronology of main events (e.g., the beginning of endospermogenesis) of the reproductive cycle.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1023/B:BIRY.0000046883.07615.e5</identifier><identifier>PMID: 15527409</identifier><language>eng</language><publisher>United States: Springer</publisher><subject>Abscisic acid ; Abscisic Acid - analogs & derivatives ; Abscisic Acid - analysis ; Abscisic Acid - immunology ; Enzyme-Linked Immunosorbent Assay ; Hormones ; Immunochemistry ; Oxidation-Reduction ; Plant tissues ; Reproductive cycle ; Taraxacum - metabolism ; Triticum - metabolism ; Wheat</subject><ispartof>Biochemistry (Moscow), 2004-10, Vol.69 (10), p.1099-1108</ispartof><rights>COPYRIGHT 2004 Springer</rights><rights>MAIK "Nauka/Interperiodica" 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-f5166636b2cf69765d6c67547d2b8339a9638f600d02e832b33993124e0be27f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15527409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blintsov, A N</creatorcontrib><creatorcontrib>Gussakovskaya, M A</creatorcontrib><title>Immunochemical approach to the problem of differential determination of natural forms of abscisic acid</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry (Mosc)</addtitle><description>An original modification of the standard ELISA procedure for differential determination of different forms of abscisic acid (ABA) is proposed. It is shown that endogenous forms of ABA may be quantitatively determined in plant tissues subjected to minimal treatment, without purification of the hormones and their chemical modification. The modification has been approved when analyzing changes in the content of different ABA forms in plant tissues differing in physiological activity. Quantitative differential determination of changes in the content of different ABA forms has been performed in ovaries of Triticum aestivum L. and Taraxacum officinale Web. in the period of activity of the ovule (from the moment of its activation to the beginning of division). It is shown that, despite the different types of reproduction in the species studied (amphimixis and apomixis), the time course of changes in the content of different forms of ABA in ovaries is similar, which is suggestive of a correlation between the activity of endogenous hormonal system and chronology of main events (e.g., the beginning of endospermogenesis) of the reproductive cycle.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - analogs & derivatives</subject><subject>Abscisic Acid - analysis</subject><subject>Abscisic Acid - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Hormones</subject><subject>Immunochemistry</subject><subject>Oxidation-Reduction</subject><subject>Plant tissues</subject><subject>Reproductive cycle</subject><subject>Taraxacum - metabolism</subject><subject>Triticum - metabolism</subject><subject>Wheat</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUFr3DAQhUVpaLZp_0IxKfTmzUiyJCu3bEjbhUChtIeehCyPugq2tZXsQ_995exCoLqMZt43w4NHyDWFLQXGb3a3u_33X1tYXyPblm9BSSq2KF6RDZXQ1hwaeE02RZc100pfkrc5P5WWgeZvyCUVgqkG9Ib4_TguU3QHHIOzQ2WPxxStO1RzrOYDVqXrBhyr6Ks-eI8JpzkUrscZ0xgmO4c4rWr5LakIPqYxrwPbZRdycJV1oX9HLrwdMr4_1yvy8_PDj_uv9eO3L_v7u8facQVz7QWVUnLZMeelVlL00kklGtWzruVcWy156yVADwxbzroy05yyBqFDpjy_Ip9Od4vvPwvm2YwhOxwGO2FcspEKmlZQKOD1f-BTXNJUvBlFNXDBJS3QxxP02w5owuTjnKxbL5o7KriQTcNX6vZEuRRzTujNMYXRpr-GglkDMzuzBmZeAjPPgRkUZfnD2cfSjdi_rJ4T4v8A3VKQnw</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Blintsov, A N</creator><creator>Gussakovskaya, M A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Immunochemical approach to the problem of differential determination of natural forms of abscisic acid</title><author>Blintsov, A N ; 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| subjects | Abscisic acid Abscisic Acid - analogs & derivatives Abscisic Acid - analysis Abscisic Acid - immunology Enzyme-Linked Immunosorbent Assay Hormones Immunochemistry Oxidation-Reduction Plant tissues Reproductive cycle Taraxacum - metabolism Triticum - metabolism Wheat |
| title | Immunochemical approach to the problem of differential determination of natural forms of abscisic acid |
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