Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment
Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO 2 mi...
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| Veröffentlicht in: | Analytica chimica acta 2009-04, Vol.638 (1), p.51-57 |
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| container_title | Analytica chimica acta |
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| creator | Leitner, Alexander Sturm, Martin Smått, Jan-Henrik Järn, Mikael Lindén, Mika Mechtler, Karl Lindner, Wolfgang |
| description | Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO
2 microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography–mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters. |
| doi_str_mv | 10.1016/j.aca.2009.01.063 |
| format | Article |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Affinity Chromatography, Liquid Exact sciences and technology Mass Spectrometry Metal oxide affinity chromatography Microspheres Nanocasting Nanotechnology Other chromatographic methods Particle Size Phosphopeptide enrichment Phosphopeptides - analysis Phosphopeptides - isolation & purification Phosphorylation Porosity Spectrometric and optical methods Tin Compounds - chemistry Tin dioxide |
| title | Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment |
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