Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging
The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induce...
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| Veröffentlicht in: | Optics express 2009-03, Vol.17 (6), p.4868-4878 |
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| container_title | Optics express |
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| creator | Matteini, Paolo Ratto, Fulvio Rossi, Francesca Cicchi, Riccardo Stringari, Chiara Kapsokalyvas, Dimitrios Pavone, Francesco S Pini, Roberto |
| description | The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues. |
| doi_str_mv | 10.1364/oe.17.004868 |
| format | Article |
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Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. 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Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues.</description><subject>Animals</subject><subject>Collagen - analysis</subject><subject>Collagen - radiation effects</subject><subject>Collagen - ultrastructure</subject><subject>Cornea - cytology</subject><subject>Cornea - radiation effects</subject><subject>Cornea - ultrastructure</subject><subject>Fourier Analysis</subject><subject>Lasers</subject><subject>Light</subject><subject>Sus scrofa</subject><subject>Temperature</subject><issn>1094-4087</issn><issn>1094-4087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM9LwzAcxYMobk5vnqUnT3YmTZomRxlzEwYT_HENafrNVmmbmbSD_fdGNtDTe1_48Hjfh9AtwVNCOXt0MCXFFGMmuDhDY4IlSxkWxfk_P0JXIXxhTFghi0s0IjKTVBIxRp-vW9e7fgu-1U1zSOuuGgxUSVUH5yvw0e5034PvQuJsYpzvQDdRm0ZvoEs87OMdqfKQvC0XSd3qTd1trtGF1U2Am5NO0Mfz_H22TFfrxcvsaZUaKmmfCsN4llmey6zSRAprNdWW6arIZSm0oKXRpqQ55zkGHsUApsbqMqec0PjDBN0fc3fefQ8QetXWwUAs14EbguIFZoxiHsGHI2i8C8GDVTsfu_qDIlj97qjWc0UKddwx4nen3KFsofqDT8PRH9wjbqU</recordid><startdate>20090316</startdate><enddate>20090316</enddate><creator>Matteini, Paolo</creator><creator>Ratto, Fulvio</creator><creator>Rossi, Francesca</creator><creator>Cicchi, Riccardo</creator><creator>Stringari, Chiara</creator><creator>Kapsokalyvas, Dimitrios</creator><creator>Pavone, Francesco S</creator><creator>Pini, Roberto</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090316</creationdate><title>Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging</title><author>Matteini, Paolo ; Ratto, Fulvio ; Rossi, Francesca ; Cicchi, Riccardo ; Stringari, Chiara ; Kapsokalyvas, Dimitrios ; Pavone, Francesco S ; Pini, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-8c4622f6592da198ffa3af4ad759b8a83bcacb356650e6566ce03cfab53613293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Collagen - analysis</topic><topic>Collagen - radiation effects</topic><topic>Collagen - ultrastructure</topic><topic>Cornea - cytology</topic><topic>Cornea - radiation effects</topic><topic>Cornea - ultrastructure</topic><topic>Fourier Analysis</topic><topic>Lasers</topic><topic>Light</topic><topic>Sus scrofa</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matteini, Paolo</creatorcontrib><creatorcontrib>Ratto, Fulvio</creatorcontrib><creatorcontrib>Rossi, Francesca</creatorcontrib><creatorcontrib>Cicchi, Riccardo</creatorcontrib><creatorcontrib>Stringari, Chiara</creatorcontrib><creatorcontrib>Kapsokalyvas, Dimitrios</creatorcontrib><creatorcontrib>Pavone, Francesco S</creatorcontrib><creatorcontrib>Pini, Roberto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Optics express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matteini, Paolo</au><au>Ratto, Fulvio</au><au>Rossi, Francesca</au><au>Cicchi, Riccardo</au><au>Stringari, Chiara</au><au>Kapsokalyvas, Dimitrios</au><au>Pavone, Francesco S</au><au>Pini, Roberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging</atitle><jtitle>Optics express</jtitle><addtitle>Opt Express</addtitle><date>2009-03-16</date><risdate>2009</risdate><volume>17</volume><issue>6</issue><spage>4868</spage><epage>4878</epage><pages>4868-4878</pages><issn>1094-4087</issn><eissn>1094-4087</eissn><abstract>The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. 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| source | MEDLINE; Directory of Open Access Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Collagen - analysis Collagen - radiation effects Collagen - ultrastructure Cornea - cytology Cornea - radiation effects Cornea - ultrastructure Fourier Analysis Lasers Light Sus scrofa Temperature |
| title | Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging |
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