Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols
To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transforman...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2009-04, Vol.82 (5), p.837-845 |
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| creator | Ohto, Chikara Muramatsu, Masayoshi Obata, Shusei Sakuradani, Eiji Shimizu, Sakayu |
| description | To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated as to the production of these alcohols. Overexpression of the gene encoding hydroxymethylglutaryl (HMG)-CoA reductase was most effective among the genes tested. A derivative of S. cerevisiae ATCC 200589, which was selected through screening, was found to be the most suitable host for the production. On cultivation of the resultant transformant, in which the HMG-CoA reductase gene was overexpressed, in a 5-liter bench-scale jar fermenter for 7 d, the production of FOH, NOH, and GGOH reached 145.7, 98.8, and 2.46 mg/l, respectively. |
| doi_str_mv | 10.1007/s00253-008-1807-5 |
| format | Article |
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Overexpression of the gene encoding hydroxymethylglutaryl (HMG)-CoA reductase was most effective among the genes tested. A derivative of S. cerevisiae ATCC 200589, which was selected through screening, was found to be the most suitable host for the production. On cultivation of the resultant transformant, in which the HMG-CoA reductase gene was overexpressed, in a 5-liter bench-scale jar fermenter for 7 d, the production of FOH, NOH, and GGOH reached 145.7, 98.8, and 2.46 mg/l, respectively.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-008-1807-5</identifier><identifier>PMID: 19083230</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Alcohol ; Biological and medical sciences ; Bioreactors ; Biosynthesis ; Biotechnological Products and Process Engineering ; Biotechnology ; Chemical synthesis ; Cloning ; Enzymes ; farnesol ; Fatty Alcohols - chemistry ; Fatty Alcohols - metabolism ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - biosynthesis ; Fungal Proteins - genetics ; Genes ; Genetics ; Geranylgeraniol ; Hydroxymethylglutaryl CoA Reductases - biosynthesis ; Hydroxymethylglutaryl CoA Reductases - chemistry ; Hydroxymethylglutaryl CoA Reductases - genetics ; Industrial Microbiology - methods ; Kinases ; Life Sciences ; Metabolic Networks and Pathways - genetics ; Mevalonic Acid - metabolism ; Microbial Genetics and Genomics ; Microbiology ; Nerolidol ; Oils & fats ; Prenyl alcohol ; Production ; Promoter Regions, Genetic ; Protein Structure, Tertiary ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Steroids ; Sterols ; Studies ; Terpenes - metabolism ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2009-04, Vol.82 (5), p.837-845</ispartof><rights>Springer-Verlag 2008</rights><rights>2009 INIST-CNRS</rights><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-cb526f3b8d45900a64c3a51abe9bb7131e02fc9b0460a49af406c3d6dbc18d8f3</citedby><cites>FETCH-LOGICAL-c520t-cb526f3b8d45900a64c3a51abe9bb7131e02fc9b0460a49af406c3d6dbc18d8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-008-1807-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-008-1807-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21274961$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19083230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohto, Chikara</creatorcontrib><creatorcontrib>Muramatsu, Masayoshi</creatorcontrib><creatorcontrib>Obata, Shusei</creatorcontrib><creatorcontrib>Sakuradani, Eiji</creatorcontrib><creatorcontrib>Shimizu, Sakayu</creatorcontrib><title>Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated as to the production of these alcohols. Overexpression of the gene encoding hydroxymethylglutaryl (HMG)-CoA reductase was most effective among the genes tested. A derivative of S. cerevisiae ATCC 200589, which was selected through screening, was found to be the most suitable host for the production. On cultivation of the resultant transformant, in which the HMG-CoA reductase gene was overexpressed, in a 5-liter bench-scale jar fermenter for 7 d, the production of FOH, NOH, and GGOH reached 145.7, 98.8, and 2.46 mg/l, respectively.</description><subject>Alcohol</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biosynthesis</subject><subject>Biotechnological Products and Process Engineering</subject><subject>Biotechnology</subject><subject>Chemical synthesis</subject><subject>Cloning</subject><subject>Enzymes</subject><subject>farnesol</subject><subject>Fatty Alcohols - chemistry</subject><subject>Fatty Alcohols - metabolism</subject><subject>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohto, Chikara</au><au>Muramatsu, Masayoshi</au><au>Obata, Shusei</au><au>Sakuradani, Eiji</au><au>Shimizu, Sakayu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>82</volume><issue>5</issue><spage>837</spage><epage>845</epage><pages>837-845</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated as to the production of these alcohols. Overexpression of the gene encoding hydroxymethylglutaryl (HMG)-CoA reductase was most effective among the genes tested. A derivative of S. cerevisiae ATCC 200589, which was selected through screening, was found to be the most suitable host for the production. On cultivation of the resultant transformant, in which the HMG-CoA reductase gene was overexpressed, in a 5-liter bench-scale jar fermenter for 7 d, the production of FOH, NOH, and GGOH reached 145.7, 98.8, and 2.46 mg/l, respectively.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19083230</pmid><doi>10.1007/s00253-008-1807-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 2009-04, Vol.82 (5), p.837-845 |
| issn | 0175-7598 1432-0614 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Alcohol Biological and medical sciences Bioreactors Biosynthesis Biotechnological Products and Process Engineering Biotechnology Chemical synthesis Cloning Enzymes farnesol Fatty Alcohols - chemistry Fatty Alcohols - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins - biosynthesis Fungal Proteins - genetics Genes Genetics Geranylgeraniol Hydroxymethylglutaryl CoA Reductases - biosynthesis Hydroxymethylglutaryl CoA Reductases - chemistry Hydroxymethylglutaryl CoA Reductases - genetics Industrial Microbiology - methods Kinases Life Sciences Metabolic Networks and Pathways - genetics Mevalonic Acid - metabolism Microbial Genetics and Genomics Microbiology Nerolidol Oils & fats Prenyl alcohol Production Promoter Regions, Genetic Protein Structure, Tertiary Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Steroids Sterols Studies Terpenes - metabolism Yeast |
| title | Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols |
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