MTP1‐dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn‐hyperaccumulating plants
Summary The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goes...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2009-03, Vol.57 (6), p.1116-1127 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Gustin, Jeffery L. Loureiro, Marcello E. Kim, Donggiun Na, Gunnam Tikhonova, Marina Salt, David E. |
| description | Summary
The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot‐specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response. |
| doi_str_mv | 10.1111/j.1365-313X.2008.03754.x |
| format | Article |
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The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot‐specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2008.03754.x</identifier><identifier>PMID: 19054361</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Biological and medical sciences ; Botany ; Cation Transport Proteins - genetics ; Cation Transport Proteins - metabolism ; Deficiencies. Phytotoxicity of elements. Salinity ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; General agronomy. Plant production ; grafting ; hyperaccumulation ; Membranes ; Metals ; micronutrient ; Molecular Sequence Data ; MTP ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Shoots - genetics ; Plant Shoots - metabolism ; Proteins ; Sequence Alignment ; Soil-plant relationships. Soil fertility. Fertilization. Amendments ; Thlaspi ; Thlaspi - genetics ; Thlaspi - metabolism ; Vacuoles - genetics ; Vacuoles - metabolism ; Zinc ; Zinc - metabolism ; Zn deficiency</subject><ispartof>The Plant journal : for cell and molecular biology, 2009-03, Vol.57 (6), p.1116-1127</ispartof><rights>2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Journal compilation © 2009 Blackwell Publishing Ltd and the Society for Experimental Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4744-69f460b067769f64913e0fafefeffb9672e726b891bbe0f15f25a2b0a44824783</citedby><cites>FETCH-LOGICAL-c4744-69f460b067769f64913e0fafefeffb9672e726b891bbe0f15f25a2b0a44824783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2008.03754.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2008.03754.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21213134$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19054361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gustin, Jeffery L.</creatorcontrib><creatorcontrib>Loureiro, Marcello E.</creatorcontrib><creatorcontrib>Kim, Donggiun</creatorcontrib><creatorcontrib>Na, Gunnam</creatorcontrib><creatorcontrib>Tikhonova, Marina</creatorcontrib><creatorcontrib>Salt, David E.</creatorcontrib><title>MTP1‐dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn‐hyperaccumulating plants</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot‐specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Biological and medical sciences</subject><subject>Botany</subject><subject>Cation Transport Proteins - genetics</subject><subject>Cation Transport Proteins - metabolism</subject><subject>Deficiencies. Phytotoxicity of elements. Salinity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>General agronomy. Plant production</subject><subject>grafting</subject><subject>hyperaccumulation</subject><subject>Membranes</subject><subject>Metals</subject><subject>micronutrient</subject><subject>Molecular Sequence Data</subject><subject>MTP</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Shoots - genetics</subject><subject>Plant Shoots - metabolism</subject><subject>Proteins</subject><subject>Sequence Alignment</subject><subject>Soil-plant relationships. Soil fertility. Fertilization. Amendments</subject><subject>Thlaspi</subject><subject>Thlaspi - genetics</subject><subject>Thlaspi - metabolism</subject><subject>Vacuoles - genetics</subject><subject>Vacuoles - metabolism</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><subject>Zn deficiency</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi1ERZfCKyALCW5J_S92cuCAqgJFRfSwSIiL5STONqusHewYujfuXHjGPgmT3WWROGEfPPL8ZuazP4QwJTmFdb7OKZdFxin_nDNCypxwVYj87gFaHBMP0YJUkmRKUHaKHse4JoQqLsUjdEorUggu6QL9_LC8ofc_frV2tK61bsJfHI72a7JxCmbqvcO9mzyOt95P-Jtpkh9sxDGtVkBE3CYz4LC7691cO0EcjGssNq7FpmnSJg1_GgEAs263IyDHjFvhcTBuik_QSWeGaJ8ezjP06c3l8uJddv3x7dXF6-usEUqITFadkKQmUikIpagot6QznYXd1ZVUzCom67KidQ0JWnSsMKwmRoiSCVXyM_Ry33cMfvdQveljYwcQYX2KWioiKKEMwOf_gGufggNtmlEuZFWyAqByDzXBxxhsp8fQb0zYakr07JZe69kUPZuiZ7f0zi19B6XPDv1TvbHt38KDPQC8OAAmNmbo5o_t45FjFGSAEOBe7bnv_WC3_y1AL2_ezxH_DTf1tJc</recordid><startdate>200903</startdate><enddate>200903</enddate><creator>Gustin, Jeffery L.</creator><creator>Loureiro, Marcello E.</creator><creator>Kim, Donggiun</creator><creator>Na, Gunnam</creator><creator>Tikhonova, Marina</creator><creator>Salt, David E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200903</creationdate><title>MTP1‐dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn‐hyperaccumulating plants</title><author>Gustin, Jeffery L. ; Loureiro, Marcello E. ; Kim, Donggiun ; Na, Gunnam ; Tikhonova, Marina ; Salt, David E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4744-69f460b067769f64913e0fafefeffb9672e726b891bbe0f15f25a2b0a44824783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Biological and medical sciences</topic><topic>Botany</topic><topic>Cation Transport Proteins - genetics</topic><topic>Cation Transport Proteins - metabolism</topic><topic>Deficiencies. Phytotoxicity of elements. Salinity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>General agronomy. Plant production</topic><topic>grafting</topic><topic>hyperaccumulation</topic><topic>Membranes</topic><topic>Metals</topic><topic>micronutrient</topic><topic>Molecular Sequence Data</topic><topic>MTP</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Shoots - genetics</topic><topic>Plant Shoots - metabolism</topic><topic>Proteins</topic><topic>Sequence Alignment</topic><topic>Soil-plant relationships. Soil fertility. Fertilization. Amendments</topic><topic>Thlaspi</topic><topic>Thlaspi - genetics</topic><topic>Thlaspi - metabolism</topic><topic>Vacuoles - genetics</topic><topic>Vacuoles - metabolism</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><topic>Zn deficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gustin, Jeffery L.</creatorcontrib><creatorcontrib>Loureiro, Marcello E.</creatorcontrib><creatorcontrib>Kim, Donggiun</creatorcontrib><creatorcontrib>Na, Gunnam</creatorcontrib><creatorcontrib>Tikhonova, Marina</creatorcontrib><creatorcontrib>Salt, David E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gustin, Jeffery L.</au><au>Loureiro, Marcello E.</au><au>Kim, Donggiun</au><au>Na, Gunnam</au><au>Tikhonova, Marina</au><au>Salt, David E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MTP1‐dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn‐hyperaccumulating plants</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2009-03</date><risdate>2009</risdate><volume>57</volume><issue>6</issue><spage>1116</spage><epage>1127</epage><pages>1116-1127</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot‐specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19054361</pmid><doi>10.1111/j.1365-313X.2008.03754.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection) |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence Arabidopsis - genetics Arabidopsis - metabolism Biological and medical sciences Botany Cation Transport Proteins - genetics Cation Transport Proteins - metabolism Deficiencies. Phytotoxicity of elements. Salinity Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant General agronomy. Plant production grafting hyperaccumulation Membranes Metals micronutrient Molecular Sequence Data MTP Plant Proteins - genetics Plant Proteins - metabolism Plant Shoots - genetics Plant Shoots - metabolism Proteins Sequence Alignment Soil-plant relationships. Soil fertility. Fertilization. Amendments Thlaspi Thlaspi - genetics Thlaspi - metabolism Vacuoles - genetics Vacuoles - metabolism Zinc Zinc - metabolism Zn deficiency |
| title | MTP1‐dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn‐hyperaccumulating plants |
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