Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy
Patients presenting with pleural effusion of undetermined aetiology were prospectively enrolled, and an enzyme-linked immunospot (ELISPOT) assay on pleural fluid and peripheral blood was performed. Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically com...
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| Veröffentlicht in: | Clinical microbiology and infection 2009-02, Vol.15 (2), p.173-179 |
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| creator | Lee, L.-N. Chou, C.-H. Wang, J.-Y. Hsu, H.-L. Tsai, T.-H. Jan, I.-S. Hsueh, P.-R. Yang, P.-C. |
| description | Patients presenting with pleural effusion of undetermined aetiology were prospectively enrolled, and an enzyme-linked immunospot (ELISPOT) assay on pleural fluid and peripheral blood was performed. Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic. |
| doi_str_mv | 10.1111/j.1469-0691.2008.02655.x |
| format | Article |
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Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2008.02655.x</identifier><identifier>PMID: 19154487</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Bacterial diseases ; Biological and medical sciences ; Blood - immunology ; Diagnosis ; enzyme-linked immunospot assay ; Exudates and Transudates - immunology ; Female ; Human bacterial diseases ; Humans ; Immunoassay - methods ; Infectious diseases ; interferon-gamma ; Interferon-gamma - analysis ; Interferon-gamma - blood ; Male ; Medical sciences ; Middle Aged ; Pneumology ; Predictive Value of Tests ; Prospective Studies ; Respiratory system : syndromes and miscellaneous diseases ; Sensitivity and Specificity ; Tuberculosis and atypical mycobacterial infections ; Tuberculosis, Pleural - diagnosis ; tuberculous pleurisy ; Young Adult</subject><ispartof>Clinical microbiology and infection, 2009-02, Vol.15 (2), p.173-179</ispartof><rights>2009 European Society of Clinical Infectious Diseases</rights><rights>2009 The Authors. 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Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Blood - immunology</subject><subject>Diagnosis</subject><subject>enzyme-linked immunospot assay</subject><subject>Exudates and Transudates - immunology</subject><subject>Female</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Infectious diseases</subject><subject>interferon-gamma</subject><subject>Interferon-gamma - analysis</subject><subject>Interferon-gamma - blood</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pneumology</subject><subject>Predictive Value of Tests</subject><subject>Prospective Studies</subject><subject>Respiratory system : syndromes and miscellaneous diseases</subject><subject>Sensitivity and Specificity</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis, Pleural - diagnosis</subject><subject>tuberculous pleurisy</subject><subject>Young Adult</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS1ERcvAX0DewC7BzzhesIBRKUhTsQGJBZJlOzfFQx6DnUDTX4_DjMqyeGFf2d-xr89BCFNS0jxe70sqKl2QStOSEVKXhFVSlreP0MX9weNcU10XSvCv5-hpSntCCONcPEHnVFMpRK0u0LfL4W7poejC8AMaHPp-HsZ0GCdsU7ILbseIwzBBbCGOQ3Fj-97mDTx9B9wEe5PhkPDY4ml2EP3cjXPChw7mGNLyDJ21tkvw_LRu0Jf3l5-3H4rdp6uP27e7wkuuZFFZ5yhRRHqunNTaNtRS7qXk1tWsVUpJ3vCaSaZq6xrHZAtaO6IroURNNN-gV8d7D3H8OUOaTB-Sh66zA-R-TKUI14LSB0FGhNCkFhmsj6CPY0oRWnOIobdxMZSYNQKzN6vTZnXarBGYvxGY2yx9cXpjdj00_4QnzzPw8gTY5G3XRjv4kO45RhmXPE8b9ObI_Q4dLP_dgNnurtcq698d9ZCt_xUgmuQDDB6aEMFPphnDw7_5A1qtt3c</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Lee, L.-N.</creator><creator>Chou, C.-H.</creator><creator>Wang, J.-Y.</creator><creator>Hsu, H.-L.</creator><creator>Tsai, T.-H.</creator><creator>Jan, I.-S.</creator><creator>Hsueh, P.-R.</creator><creator>Yang, P.-C.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200902</creationdate><title>Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy</title><author>Lee, L.-N. ; Chou, C.-H. ; Wang, J.-Y. ; Hsu, H.-L. ; Tsai, T.-H. ; Jan, I.-S. ; Hsueh, P.-R. ; Yang, P.-C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5375-6abb10705c37b599ad1a13c553ab82f77753d3825278abdb25fe99b0964748093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Blood - immunology</topic><topic>Diagnosis</topic><topic>enzyme-linked immunospot assay</topic><topic>Exudates and Transudates - immunology</topic><topic>Female</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Infectious diseases</topic><topic>interferon-gamma</topic><topic>Interferon-gamma - analysis</topic><topic>Interferon-gamma - blood</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pneumology</topic><topic>Predictive Value of Tests</topic><topic>Prospective Studies</topic><topic>Respiratory system : syndromes and miscellaneous diseases</topic><topic>Sensitivity and Specificity</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tuberculosis, Pleural - diagnosis</topic><topic>tuberculous pleurisy</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, L.-N.</creatorcontrib><creatorcontrib>Chou, C.-H.</creatorcontrib><creatorcontrib>Wang, J.-Y.</creatorcontrib><creatorcontrib>Hsu, H.-L.</creatorcontrib><creatorcontrib>Tsai, T.-H.</creatorcontrib><creatorcontrib>Jan, I.-S.</creatorcontrib><creatorcontrib>Hsueh, P.-R.</creatorcontrib><creatorcontrib>Yang, P.-C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, L.-N.</au><au>Chou, C.-H.</au><au>Wang, J.-Y.</au><au>Hsu, H.-L.</au><au>Tsai, T.-H.</au><au>Jan, I.-S.</au><au>Hsueh, P.-R.</au><au>Yang, P.-C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2009-02</date><risdate>2009</risdate><volume>15</volume><issue>2</issue><spage>173</spage><epage>179</epage><pages>173-179</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>Patients presenting with pleural effusion of undetermined aetiology were prospectively enrolled, and an enzyme-linked immunospot (ELISPOT) assay on pleural fluid and peripheral blood was performed. Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>19154487</pmid><doi>10.1111/j.1469-0691.2008.02655.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Bacterial diseases Biological and medical sciences Blood - immunology Diagnosis enzyme-linked immunospot assay Exudates and Transudates - immunology Female Human bacterial diseases Humans Immunoassay - methods Infectious diseases interferon-gamma Interferon-gamma - analysis Interferon-gamma - blood Male Medical sciences Middle Aged Pneumology Predictive Value of Tests Prospective Studies Respiratory system : syndromes and miscellaneous diseases Sensitivity and Specificity Tuberculosis and atypical mycobacterial infections Tuberculosis, Pleural - diagnosis tuberculous pleurisy Young Adult |
| title | Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy |
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