Chromator, a novel and essential chromodomain protein interacts directly with the putative spindle matrix protein skeletor

We have used a yeast two‐hybrid interaction assay to identify Chromator, a novel chromodomain containing protein that interacts directly with the putative spindle matrix protein Skeletor. Immunocytochemistry demonstrated that Chromator and Skeletor show extensive co‐localization throughout the cell...

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Veröffentlicht in:Journal of cellular biochemistry 2004-12, Vol.93 (5), p.1033-1047
Hauptverfasser: Rath, Uttama, Wang, Dong, Ding, Yun, Xu, Ying-Zhi, Qi, Hongying, Blacketer, Melissa J., Girton, Jack, Johansen, Jørgen, Johansen, Kristen M.
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container_end_page 1047
container_issue 5
container_start_page 1033
container_title Journal of cellular biochemistry
container_volume 93
creator Rath, Uttama
Wang, Dong
Ding, Yun
Xu, Ying-Zhi
Qi, Hongying
Blacketer, Melissa J.
Girton, Jack
Johansen, Jørgen
Johansen, Kristen M.
description We have used a yeast two‐hybrid interaction assay to identify Chromator, a novel chromodomain containing protein that interacts directly with the putative spindle matrix protein Skeletor. Immunocytochemistry demonstrated that Chromator and Skeletor show extensive co‐localization throughout the cell cycle. During interphase Chromator is localized on chromosomes to interband chromatin regions in a pattern that overlaps that of Skeletor. However, during mitosis both Chromator and Skeletor detach from the chromosomes and align together in a spindle‐like structure. Deletion construct analysis in S2 cells showed that the COOH‐terminal half of Chromator without the chromodomain was sufficient for both nuclear as well as spindle localization. Analysis of P‐element mutations in the Chromator locus shows that Chromator is an essential protein. Furthermore, RNAi depletion of Chromator in S2 cells leads to abnormal microtubule spindle morphology and to chromosome segregation defects. These findings suggest that Chromator is a nuclear protein that plays a role in proper spindle dynamics during mitosis. © 2004 Wiley‐Liss, Inc.
doi_str_mv 10.1002/jcb.20243
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Cell. Biochem</addtitle><description>We have used a yeast two‐hybrid interaction assay to identify Chromator, a novel chromodomain containing protein that interacts directly with the putative spindle matrix protein Skeletor. Immunocytochemistry demonstrated that Chromator and Skeletor show extensive co‐localization throughout the cell cycle. During interphase Chromator is localized on chromosomes to interband chromatin regions in a pattern that overlaps that of Skeletor. However, during mitosis both Chromator and Skeletor detach from the chromosomes and align together in a spindle‐like structure. Deletion construct analysis in S2 cells showed that the COOH‐terminal half of Chromator without the chromodomain was sufficient for both nuclear as well as spindle localization. Analysis of P‐element mutations in the Chromator locus shows that Chromator is an essential protein. 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source Wiley Online Library - AutoHoldings Journals; MEDLINE
subjects Amino Acid Sequence
Animals
Cell Cycle - physiology
Cell Line
Cell Nucleus - metabolism
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
chromosomes
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
microtubules
mitosis
Molecular Sequence Data
Nuclear Matrix-Associated Proteins - genetics
Nuclear Matrix-Associated Proteins - metabolism
Protein Binding
RNA Interference
Spindle Apparatus - metabolism
spindle matrix
Two-Hybrid System Techniques
title Chromator, a novel and essential chromodomain protein interacts directly with the putative spindle matrix protein skeletor
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