A mouse model of human adaptive immune functions: HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice
HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice were created and their immunological potential evaluated in response to hepatitis B DNA vaccine. Every single immunized mouse developed hepatitis B virus‐specific antibodies, HLA‐DR1‐restricted helper, and HLA‐A2.1‐restricted cytolytic...
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| Veröffentlicht in: | European journal of immunology 2004-11, Vol.34 (11), p.3060-3069 |
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| container_title | European journal of immunology |
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| creator | Pajot, Anthony Michel, Marie‐Louise Fazilleau, Nicolas Pancré, Véronique Auriault, Claude Ojcius, David M. Lemonnier, François A. Lone, Yu‐Chun |
| description | HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice were created and their immunological potential evaluated in response to hepatitis B DNA vaccine. Every single immunized mouse developed hepatitis B virus‐specific antibodies, HLA‐DR1‐restricted helper, and HLA‐A2.1‐restricted cytolytic T cell responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans. These mice were specifically protected against a hepatitis B‐recombinant vaccinia virus infection with a 10,000‐fold or more reduction of the virus load at day 4 post‐challenge. These mice represent a unique in vivo experimental model for human immune function studies without any interference with mouse MHC response which dwarfed the prediction of human responses. Furthermore, they enable the complete monitoring of immune adaptative responses for preclinical screening of candidate vaccines. |
| doi_str_mv | 10.1002/eji.200425463 |
| format | Article |
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Every single immunized mouse developed hepatitis B virus‐specific antibodies, HLA‐DR1‐restricted helper, and HLA‐A2.1‐restricted cytolytic T cell responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans. These mice were specifically protected against a hepatitis B‐recombinant vaccinia virus infection with a 10,000‐fold or more reduction of the virus load at day 4 post‐challenge. These mice represent a unique in vivo experimental model for human immune function studies without any interference with mouse MHC response which dwarfed the prediction of human responses. Furthermore, they enable the complete monitoring of immune adaptative responses for preclinical screening of candidate vaccines.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.200425463</identifier><identifier>PMID: 15468058</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Viral - blood ; CTL/HTL ; Flow Cytometry ; Genes, MHC Class I - immunology ; H-2 Antigens - genetics ; H-2 Antigens - immunology ; Hepatitis B - immunology ; Hepatitis B - prevention & control ; Hepatitis B Surface Antigens - immunology ; Hepatitis B Vaccines - immunology ; Hepatitis B virus - immunology ; HLA class I and II transgenic mice ; HLA-A2 Antigen - genetics ; HLA-A2 Antigen - immunology ; HLA-DR1 Antigen - genetics ; HLA-DR1 Antigen - immunology ; Human immune response ; Human vaccine evaluation ; Humans ; H‐2 class I and II KO mice ; Immunophenotyping ; Mice ; Mice, Knockout ; Mice, Transgenic ; Models, Animal ; Molecular Sequence Data ; Vaccination ; Vaccines, DNA - immunology</subject><ispartof>European journal of immunology, 2004-11, Vol.34 (11), p.3060-3069</ispartof><rights>Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3763-dbd3f7e7ab5acc2a229e0063cfc6faf8dd5e7918984ff061c0fd1d14059da3c93</citedby><cites>FETCH-LOGICAL-c3763-dbd3f7e7ab5acc2a229e0063cfc6faf8dd5e7918984ff061c0fd1d14059da3c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Feji.200425463$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Feji.200425463$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15468058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pajot, Anthony</creatorcontrib><creatorcontrib>Michel, Marie‐Louise</creatorcontrib><creatorcontrib>Fazilleau, Nicolas</creatorcontrib><creatorcontrib>Pancré, Véronique</creatorcontrib><creatorcontrib>Auriault, Claude</creatorcontrib><creatorcontrib>Ojcius, David M.</creatorcontrib><creatorcontrib>Lemonnier, François A.</creatorcontrib><creatorcontrib>Lone, Yu‐Chun</creatorcontrib><title>A mouse model of human adaptive immune functions: HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice were created and their immunological potential evaluated in response to hepatitis B DNA vaccine. Every single immunized mouse developed hepatitis B virus‐specific antibodies, HLA‐DR1‐restricted helper, and HLA‐A2.1‐restricted cytolytic T cell responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans. These mice were specifically protected against a hepatitis B‐recombinant vaccinia virus infection with a 10,000‐fold or more reduction of the virus load at day 4 post‐challenge. These mice represent a unique in vivo experimental model for human immune function studies without any interference with mouse MHC response which dwarfed the prediction of human responses. Furthermore, they enable the complete monitoring of immune adaptative responses for preclinical screening of candidate vaccines.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>CTL/HTL</subject><subject>Flow Cytometry</subject><subject>Genes, MHC Class I - immunology</subject><subject>H-2 Antigens - genetics</subject><subject>H-2 Antigens - immunology</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B - prevention & control</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Hepatitis B virus - immunology</subject><subject>HLA class I and II transgenic mice</subject><subject>HLA-A2 Antigen - genetics</subject><subject>HLA-A2 Antigen - immunology</subject><subject>HLA-DR1 Antigen - genetics</subject><subject>HLA-DR1 Antigen - immunology</subject><subject>Human immune response</subject><subject>Human vaccine evaluation</subject><subject>Humans</subject><subject>H‐2 class I and II KO mice</subject><subject>Immunophenotyping</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mice, Transgenic</subject><subject>Models, Animal</subject><subject>Molecular Sequence Data</subject><subject>Vaccination</subject><subject>Vaccines, DNA - immunology</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOAzEQRS0EgvAoaZEruk3G3qfpIggkKBISgnrl2GMw7COsd0HpUuQD-MZ8CY4SQUczoyMd3eIQcs6gzwD4AN9snwNEPI6ScI_0WMxZELGI7ZMeAIsCLjI4IsfOvQGASGJxSI6YlzOIsx5ZDWlZdw791VjQ2tDXrpQVlVrOW_uJ1JZlVyE1XaVaW1fuio6nw_Xye8j7zL_Blm4eN9A2snIvWFlFxx45VYV0br1cTTbmL2zovarVe921tLQKT8mBkYXDs90_Ic-3o6frcTB9uJtcD6eBCtMkDPRMhybFVM5iqRSXnAsESEJlVGKkybSOMRUsE1lkDCRMgdFMswhioWWoRHhCLre786b-6NC1eWmdwqKQFfoIeZJCmKUi9WKwFVVTO9egyeeNLWWzyBnkm-y5z57_Zvf-xW64m5Wo_-xdZy-kW-HLFrj4fy0f3U_-pn8AXVWWYw</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Pajot, Anthony</creator><creator>Michel, Marie‐Louise</creator><creator>Fazilleau, Nicolas</creator><creator>Pancré, Véronique</creator><creator>Auriault, Claude</creator><creator>Ojcius, David M.</creator><creator>Lemonnier, François A.</creator><creator>Lone, Yu‐Chun</creator><general>WILEY‐VCH Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200411</creationdate><title>A mouse model of human adaptive immune functions: HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice</title><author>Pajot, Anthony ; Michel, Marie‐Louise ; Fazilleau, Nicolas ; Pancré, Véronique ; Auriault, Claude ; Ojcius, David M. ; Lemonnier, François A. ; Lone, Yu‐Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3763-dbd3f7e7ab5acc2a229e0063cfc6faf8dd5e7918984ff061c0fd1d14059da3c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>CTL/HTL</topic><topic>Flow Cytometry</topic><topic>Genes, MHC Class I - immunology</topic><topic>H-2 Antigens - genetics</topic><topic>H-2 Antigens - immunology</topic><topic>Hepatitis B - immunology</topic><topic>Hepatitis B - prevention & control</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B Vaccines - immunology</topic><topic>Hepatitis B virus - immunology</topic><topic>HLA class I and II transgenic mice</topic><topic>HLA-A2 Antigen - genetics</topic><topic>HLA-A2 Antigen - immunology</topic><topic>HLA-DR1 Antigen - genetics</topic><topic>HLA-DR1 Antigen - immunology</topic><topic>Human immune response</topic><topic>Human vaccine evaluation</topic><topic>Humans</topic><topic>H‐2 class I and II KO mice</topic><topic>Immunophenotyping</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mice, Transgenic</topic><topic>Models, Animal</topic><topic>Molecular Sequence Data</topic><topic>Vaccination</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pajot, Anthony</creatorcontrib><creatorcontrib>Michel, Marie‐Louise</creatorcontrib><creatorcontrib>Fazilleau, Nicolas</creatorcontrib><creatorcontrib>Pancré, Véronique</creatorcontrib><creatorcontrib>Auriault, Claude</creatorcontrib><creatorcontrib>Ojcius, David M.</creatorcontrib><creatorcontrib>Lemonnier, François A.</creatorcontrib><creatorcontrib>Lone, Yu‐Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pajot, Anthony</au><au>Michel, Marie‐Louise</au><au>Fazilleau, Nicolas</au><au>Pancré, Véronique</au><au>Auriault, Claude</au><au>Ojcius, David M.</au><au>Lemonnier, François A.</au><au>Lone, Yu‐Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mouse model of human adaptive immune functions: HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>2004-11</date><risdate>2004</risdate><volume>34</volume><issue>11</issue><spage>3060</spage><epage>3069</epage><pages>3060-3069</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice were created and their immunological potential evaluated in response to hepatitis B DNA vaccine. Every single immunized mouse developed hepatitis B virus‐specific antibodies, HLA‐DR1‐restricted helper, and HLA‐A2.1‐restricted cytolytic T cell responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans. These mice were specifically protected against a hepatitis B‐recombinant vaccinia virus infection with a 10,000‐fold or more reduction of the virus load at day 4 post‐challenge. These mice represent a unique in vivo experimental model for human immune function studies without any interference with mouse MHC response which dwarfed the prediction of human responses. Furthermore, they enable the complete monitoring of immune adaptative responses for preclinical screening of candidate vaccines.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag</pub><pmid>15468058</pmid><doi>10.1002/eji.200425463</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of immunology, 2004-11, Vol.34 (11), p.3060-3069 |
| issn | 0014-2980 1521-4141 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Animals Antibodies, Viral - blood CTL/HTL Flow Cytometry Genes, MHC Class I - immunology H-2 Antigens - genetics H-2 Antigens - immunology Hepatitis B - immunology Hepatitis B - prevention & control Hepatitis B Surface Antigens - immunology Hepatitis B Vaccines - immunology Hepatitis B virus - immunology HLA class I and II transgenic mice HLA-A2 Antigen - genetics HLA-A2 Antigen - immunology HLA-DR1 Antigen - genetics HLA-DR1 Antigen - immunology Human immune response Human vaccine evaluation Humans H‐2 class I and II KO mice Immunophenotyping Mice Mice, Knockout Mice, Transgenic Models, Animal Molecular Sequence Data Vaccination Vaccines, DNA - immunology |
| title | A mouse model of human adaptive immune functions: HLA‐A2.1‐/HLA‐DR1‐transgenic H‐2 class I‐/class II‐knockout mice |
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