Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines
Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated. In the present study, we investigated how α-defen...
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| Veröffentlicht in: | Journal of dermatological science 2004-11, Vol.36 (2), p.87-95 |
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| container_title | Journal of dermatological science |
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| creator | Nishimura, Michiko Abiko, Yoshihiro Kurashige, Yoshihito Takeshima, Maiko Yamazaki, Mami Kusano, Kaoru Saitoh, Masato Nakashima, Keisuke Inoue, Takashi Kaku, Tohru |
| description | Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated.
In the present study, we investigated how α-defensin (HNP-1) and β-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.
Cell proliferation was assessed by the direct cell counting and XTT assay.
We found that α-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, β-defensins have little effect on these cells at any concentration, suggesting that β-defensins may have no adverse effects on the host.
Therefore, in terms of host response β-defensins may be more suitable antimicrobial agents for clinical applications than α-defensins. |
| doi_str_mv | 10.1016/j.jdermsci.2004.07.001 |
| format | Article |
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In the present study, we investigated how α-defensin (HNP-1) and β-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.
Cell proliferation was assessed by the direct cell counting and XTT assay.
We found that α-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, β-defensins have little effect on these cells at any concentration, suggesting that β-defensins may have no adverse effects on the host.
Therefore, in terms of host response β-defensins may be more suitable antimicrobial agents for clinical applications than α-defensins.</description><identifier>ISSN: 0923-1811</identifier><identifier>EISSN: 1873-569X</identifier><identifier>DOI: 10.1016/j.jdermsci.2004.07.001</identifier><identifier>PMID: 15519138</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>alpha-Defensins - administration & dosage ; alpha-Defensins - pharmacology ; Animals ; Antimicrobial peptide ; beta-Defensins - pharmacology ; Carcinoma, Squamous Cell - pathology ; Cell Count ; Cell culture ; Cell Division - drug effects ; Cell Line, Tumor ; Defensin ; Defensins ; Dose-Response Relationship, Drug ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Gingiva - cytology ; Host response ; Humans ; Oral ; Skin ; Swine</subject><ispartof>Journal of dermatological science, 2004-11, Vol.36 (2), p.87-95</ispartof><rights>2004 Japanese Society for Investigative Dermatology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-bba5a38e335858a85dbbfec40f31e098574eba1362f847e721951a38239407e3</citedby><cites>FETCH-LOGICAL-c483t-bba5a38e335858a85dbbfec40f31e098574eba1362f847e721951a38239407e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jdermsci.2004.07.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15519138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishimura, Michiko</creatorcontrib><creatorcontrib>Abiko, Yoshihiro</creatorcontrib><creatorcontrib>Kurashige, Yoshihito</creatorcontrib><creatorcontrib>Takeshima, Maiko</creatorcontrib><creatorcontrib>Yamazaki, Mami</creatorcontrib><creatorcontrib>Kusano, Kaoru</creatorcontrib><creatorcontrib>Saitoh, Masato</creatorcontrib><creatorcontrib>Nakashima, Keisuke</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><creatorcontrib>Kaku, Tohru</creatorcontrib><title>Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines</title><title>Journal of dermatological science</title><addtitle>J Dermatol Sci</addtitle><description>Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated.
In the present study, we investigated how α-defensin (HNP-1) and β-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.
Cell proliferation was assessed by the direct cell counting and XTT assay.
We found that α-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, β-defensins have little effect on these cells at any concentration, suggesting that β-defensins may have no adverse effects on the host.
Therefore, in terms of host response β-defensins may be more suitable antimicrobial agents for clinical applications than α-defensins.</description><subject>alpha-Defensins - administration & dosage</subject><subject>alpha-Defensins - pharmacology</subject><subject>Animals</subject><subject>Antimicrobial peptide</subject><subject>beta-Defensins - pharmacology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Count</subject><subject>Cell culture</subject><subject>Cell Division - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Defensin</subject><subject>Defensins</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Gingiva - cytology</subject><subject>Host response</subject><subject>Humans</subject><subject>Oral</subject><subject>Skin</subject><subject>Swine</subject><issn>0923-1811</issn><issn>1873-569X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFP3DAQhS1UBNuFv4B86qlJx3GcOD2BEC1ISFz2wM1ynLHwNomDnSD1wH-vt1nUY0_WeN6befMRcsUgZ8Cqb_t832EYonF5AVDmUOcA7IRsmKx5Jqrm-RPZQFPwjEnGzsnnGPcAIIqyOSPnTAjWMC435P3OWjQz9ZZ2aHGMbqQTTrPrMFI_Ulx-6fDbz85Qg30fv9MpuCF9UZzc_IK90_3a-Uqta4Nvex3nSPXY0fi66MEv8W-fGh2MG_2g17J3I8YLcmp1H_Hy-G7J7sfd7vY-e3z6-XB785iZUvI5a1stNJfIuZBCaim6tk2pS7CcITRS1CW2mvGqsLKssS5YI1gyFLwpoUa-JV_WsVPwrwvGWQ0uHlLoEVM-VdXAS1FBElar0AQfY0CrjtcqBurAXe3VB3d14K6gVol7Ml4dNyztgN0_2xF0ElyvAkxnvjkMKo3A0WDnQuKvOu_-t-MPUsOZvw</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Nishimura, Michiko</creator><creator>Abiko, Yoshihiro</creator><creator>Kurashige, Yoshihito</creator><creator>Takeshima, Maiko</creator><creator>Yamazaki, Mami</creator><creator>Kusano, Kaoru</creator><creator>Saitoh, Masato</creator><creator>Nakashima, Keisuke</creator><creator>Inoue, Takashi</creator><creator>Kaku, Tohru</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines</title><author>Nishimura, Michiko ; Abiko, Yoshihiro ; Kurashige, Yoshihito ; Takeshima, Maiko ; Yamazaki, Mami ; Kusano, Kaoru ; Saitoh, Masato ; Nakashima, Keisuke ; Inoue, Takashi ; Kaku, Tohru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-bba5a38e335858a85dbbfec40f31e098574eba1362f847e721951a38239407e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>alpha-Defensins - administration & dosage</topic><topic>alpha-Defensins - pharmacology</topic><topic>Animals</topic><topic>Antimicrobial peptide</topic><topic>beta-Defensins - pharmacology</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Count</topic><topic>Cell culture</topic><topic>Cell Division - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Defensin</topic><topic>Defensins</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Gingiva - cytology</topic><topic>Host response</topic><topic>Humans</topic><topic>Oral</topic><topic>Skin</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishimura, Michiko</creatorcontrib><creatorcontrib>Abiko, Yoshihiro</creatorcontrib><creatorcontrib>Kurashige, Yoshihito</creatorcontrib><creatorcontrib>Takeshima, Maiko</creatorcontrib><creatorcontrib>Yamazaki, Mami</creatorcontrib><creatorcontrib>Kusano, Kaoru</creatorcontrib><creatorcontrib>Saitoh, Masato</creatorcontrib><creatorcontrib>Nakashima, Keisuke</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><creatorcontrib>Kaku, Tohru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dermatological science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishimura, Michiko</au><au>Abiko, Yoshihiro</au><au>Kurashige, Yoshihito</au><au>Takeshima, Maiko</au><au>Yamazaki, Mami</au><au>Kusano, Kaoru</au><au>Saitoh, Masato</au><au>Nakashima, Keisuke</au><au>Inoue, Takashi</au><au>Kaku, Tohru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines</atitle><jtitle>Journal of dermatological science</jtitle><addtitle>J Dermatol Sci</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>36</volume><issue>2</issue><spage>87</spage><epage>95</epage><pages>87-95</pages><issn>0923-1811</issn><eissn>1873-569X</eissn><abstract>Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated.
In the present study, we investigated how α-defensin (HNP-1) and β-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.
Cell proliferation was assessed by the direct cell counting and XTT assay.
We found that α-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, β-defensins have little effect on these cells at any concentration, suggesting that β-defensins may have no adverse effects on the host.
Therefore, in terms of host response β-defensins may be more suitable antimicrobial agents for clinical applications than α-defensins.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>15519138</pmid><doi>10.1016/j.jdermsci.2004.07.001</doi><tpages>9</tpages></addata></record> |
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| subjects | alpha-Defensins - administration & dosage alpha-Defensins - pharmacology Animals Antimicrobial peptide beta-Defensins - pharmacology Carcinoma, Squamous Cell - pathology Cell Count Cell culture Cell Division - drug effects Cell Line, Tumor Defensin Defensins Dose-Response Relationship, Drug Epithelial Cells - cytology Epithelial Cells - drug effects Fibroblasts - cytology Fibroblasts - drug effects Gingiva - cytology Host response Humans Oral Skin Swine |
| title | Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines |
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