Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts
Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases (MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the characteristic changes of photoaging in skin. Inhibition of induction of MMPs is suggested to alleviate photoaging induced by UV irradiatio...
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| Veröffentlicht in: | International journal of molecular medicine 2009-04, Vol.23 (4), p.555-560 |
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| container_title | International journal of molecular medicine |
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| creator | Yang, Bo Ji, Chao Kang, Jian Chen, Wenqi Bi, Zhigang Wan, Yinsheng |
| description | Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases
(MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the
characteristic changes of photoaging in skin. Inhibition of induction of MMPs
is suggested to alleviate photoaging induced by UV irradiation. Zeatin, purified
from Zea mays, is a member of the cytokinin group of plant growth factors, the
activity of which is attributed to its more stable trans form. In this study,
we investigated the effect of trans-Zeatin on UVB-induced matrix metalloproteinase-1
(MMP-1) expression in cultured human skin fibroblasts (HSFs) and studied the mechanisms
of its actions. We found that pretreatment with trans-Zeatin significantly inhibits
UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner.
We also observed that trans-Zeatin inhibits UVB-induced phosphorylation of ERK,
JNK and p38 MAP kinases (MAPKs) dose-dependently. As expected, PD98059, an ERK
inhibitor, SP600125, a JNK inhibitor and SB203580, a p38 MAPK inhibitor effectively
inhibit UVB-induced phosphorylation of ERK, JNK and p38 MAPKs, respectively. Moreover,
the inhibitory mechanism of trans-Zeatin was further demonstrated in MMP-1 secretion
using MAPK-specific inhibitors. PD98059, SP600125 and SB203580 suppressed UVB-induced
MMP-1 secretion, which is consistent with the above results. Collectively, our
results suggest that trans-Zeatin inhibits UVB-induced MMP-1 expression, which
may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways in
HSFs. Trans-Zeatin is a potential agent for the management of skin photoaging |
| doi_str_mv | 10.3892/ijmm_00000164 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67027948</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67027948</sourcerecordid><originalsourceid>FETCH-LOGICAL-c432t-28e185b952741d2dc74901574e98445936575079f7bc80d3cd24a4d8b2d4126d3</originalsourceid><addsrcrecordid>eNpVkTtPwzAUhT2AaHmMrMgTCwr4GdsjIF5SEQwtA0vkxC4YEqf4Jgj49aS0CHGWO5xPR1fnILRPyTHXhp2El6YpyFI0FxtoTClRGVcyH6FtgBdCmBRGb6ERNUxrwvkYfU2TjZA9etuFiEN8DmXoAM8ezrIQXV95hxvbpfCBG9_Zum4Xqe18iBZ8RrH_WCQPENqI34PFt6f3-PXHwxCeoq1DfBoy8XPf2IhhsPA8lKktawsd7KLNua3B763vDppdXkzPr7PJ3dXN-ekkqwRnXca0p1qWRjIlqGOuUsIQKpXwRgshDc-lkkSZuSorTRyvHBNWOF0yJyjLHd9Bh6vc4fW33kNXNAEqX9c2-raHIleEKSP0AGYrsEotQPLzYpFCY9NnQUmxLLj4V_DAH6yD-7Lx7o9etzsARysAFja64Fr4Y36nYVzIQTnh35q2hyk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67027948</pqid></control><display><type>article</type><title>Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Yang, Bo ; Ji, Chao ; Kang, Jian ; Chen, Wenqi ; Bi, Zhigang ; Wan, Yinsheng</creator><creatorcontrib>Yang, Bo ; Ji, Chao ; Kang, Jian ; Chen, Wenqi ; Bi, Zhigang ; Wan, Yinsheng</creatorcontrib><description>Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases
(MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the
characteristic changes of photoaging in skin. Inhibition of induction of MMPs
is suggested to alleviate photoaging induced by UV irradiation. Zeatin, purified
from Zea mays, is a member of the cytokinin group of plant growth factors, the
activity of which is attributed to its more stable trans form. In this study,
we investigated the effect of trans-Zeatin on UVB-induced matrix metalloproteinase-1
(MMP-1) expression in cultured human skin fibroblasts (HSFs) and studied the mechanisms
of its actions. We found that pretreatment with trans-Zeatin significantly inhibits
UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner.
We also observed that trans-Zeatin inhibits UVB-induced phosphorylation of ERK,
JNK and p38 MAP kinases (MAPKs) dose-dependently. As expected, PD98059, an ERK
inhibitor, SP600125, a JNK inhibitor and SB203580, a p38 MAPK inhibitor effectively
inhibit UVB-induced phosphorylation of ERK, JNK and p38 MAPKs, respectively. Moreover,
the inhibitory mechanism of trans-Zeatin was further demonstrated in MMP-1 secretion
using MAPK-specific inhibitors. PD98059, SP600125 and SB203580 suppressed UVB-induced
MMP-1 secretion, which is consistent with the above results. Collectively, our
results suggest that trans-Zeatin inhibits UVB-induced MMP-1 expression, which
may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways in
HSFs. Trans-Zeatin is a potential agent for the management of skin photoaging</description><identifier>ISSN: 1107-3756</identifier><identifier>DOI: 10.3892/ijmm_00000164</identifier><identifier>PMID: 19288033</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Anthracenes - pharmacology ; Blotting, Western ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Enzyme Inhibitors - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - radiation effects ; Flavonoids - pharmacology ; Humans ; Imidazoles - pharmacology ; Infant, Newborn ; MAP Kinase Signaling System - drug effects ; MAP Kinase Signaling System - radiation effects ; Matrix Metalloproteinase 1 - metabolism ; Mitogen-Activated Protein Kinases - antagonists & inhibitors ; Mitogen-Activated Protein Kinases - metabolism ; Plant Growth Regulators - pharmacology ; Pyridines - pharmacology ; Skin - cytology ; Ultraviolet Rays ; Zeatin - chemistry ; Zeatin - pharmacology</subject><ispartof>International journal of molecular medicine, 2009-04, Vol.23 (4), p.555-560</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-28e185b952741d2dc74901574e98445936575079f7bc80d3cd24a4d8b2d4126d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19288033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Ji, Chao</creatorcontrib><creatorcontrib>Kang, Jian</creatorcontrib><creatorcontrib>Chen, Wenqi</creatorcontrib><creatorcontrib>Bi, Zhigang</creatorcontrib><creatorcontrib>Wan, Yinsheng</creatorcontrib><title>Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases
(MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the
characteristic changes of photoaging in skin. Inhibition of induction of MMPs
is suggested to alleviate photoaging induced by UV irradiation. Zeatin, purified
from Zea mays, is a member of the cytokinin group of plant growth factors, the
activity of which is attributed to its more stable trans form. In this study,
we investigated the effect of trans-Zeatin on UVB-induced matrix metalloproteinase-1
(MMP-1) expression in cultured human skin fibroblasts (HSFs) and studied the mechanisms
of its actions. We found that pretreatment with trans-Zeatin significantly inhibits
UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner.
We also observed that trans-Zeatin inhibits UVB-induced phosphorylation of ERK,
JNK and p38 MAP kinases (MAPKs) dose-dependently. As expected, PD98059, an ERK
inhibitor, SP600125, a JNK inhibitor and SB203580, a p38 MAPK inhibitor effectively
inhibit UVB-induced phosphorylation of ERK, JNK and p38 MAPKs, respectively. Moreover,
the inhibitory mechanism of trans-Zeatin was further demonstrated in MMP-1 secretion
using MAPK-specific inhibitors. PD98059, SP600125 and SB203580 suppressed UVB-induced
MMP-1 secretion, which is consistent with the above results. Collectively, our
results suggest that trans-Zeatin inhibits UVB-induced MMP-1 expression, which
may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways in
HSFs. Trans-Zeatin is a potential agent for the management of skin photoaging</description><subject>Anthracenes - pharmacology</subject><subject>Blotting, Western</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - radiation effects</subject><subject>Flavonoids - pharmacology</subject><subject>Humans</subject><subject>Imidazoles - pharmacology</subject><subject>Infant, Newborn</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>MAP Kinase Signaling System - radiation effects</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Pyridines - pharmacology</subject><subject>Skin - cytology</subject><subject>Ultraviolet Rays</subject><subject>Zeatin - chemistry</subject><subject>Zeatin - pharmacology</subject><issn>1107-3756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkTtPwzAUhT2AaHmMrMgTCwr4GdsjIF5SEQwtA0vkxC4YEqf4Jgj49aS0CHGWO5xPR1fnILRPyTHXhp2El6YpyFI0FxtoTClRGVcyH6FtgBdCmBRGb6ERNUxrwvkYfU2TjZA9etuFiEN8DmXoAM8ezrIQXV95hxvbpfCBG9_Zum4Xqe18iBZ8RrH_WCQPENqI34PFt6f3-PXHwxCeoq1DfBoy8XPf2IhhsPA8lKktawsd7KLNua3B763vDppdXkzPr7PJ3dXN-ekkqwRnXca0p1qWRjIlqGOuUsIQKpXwRgshDc-lkkSZuSorTRyvHBNWOF0yJyjLHd9Bh6vc4fW33kNXNAEqX9c2-raHIleEKSP0AGYrsEotQPLzYpFCY9NnQUmxLLj4V_DAH6yD-7Lx7o9etzsARysAFja64Fr4Y36nYVzIQTnh35q2hyk</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Yang, Bo</creator><creator>Ji, Chao</creator><creator>Kang, Jian</creator><creator>Chen, Wenqi</creator><creator>Bi, Zhigang</creator><creator>Wan, Yinsheng</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts</title><author>Yang, Bo ; Ji, Chao ; Kang, Jian ; Chen, Wenqi ; Bi, Zhigang ; Wan, Yinsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-28e185b952741d2dc74901574e98445936575079f7bc80d3cd24a4d8b2d4126d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anthracenes - pharmacology</topic><topic>Blotting, Western</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - radiation effects</topic><topic>Flavonoids - pharmacology</topic><topic>Humans</topic><topic>Imidazoles - pharmacology</topic><topic>Infant, Newborn</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>MAP Kinase Signaling System - radiation effects</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinases - antagonists & inhibitors</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Pyridines - pharmacology</topic><topic>Skin - cytology</topic><topic>Ultraviolet Rays</topic><topic>Zeatin - chemistry</topic><topic>Zeatin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Ji, Chao</creatorcontrib><creatorcontrib>Kang, Jian</creatorcontrib><creatorcontrib>Chen, Wenqi</creatorcontrib><creatorcontrib>Bi, Zhigang</creatorcontrib><creatorcontrib>Wan, Yinsheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Bo</au><au>Ji, Chao</au><au>Kang, Jian</au><au>Chen, Wenqi</au><au>Bi, Zhigang</au><au>Wan, Yinsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>23</volume><issue>4</issue><spage>555</spage><epage>560</epage><pages>555-560</pages><issn>1107-3756</issn><abstract>Ultraviolet (UV) irradiation induces the expression of matrix metalloproteinases
(MMPs), disturbing the metabolism of extracellular matrix (ECM), and causes the
characteristic changes of photoaging in skin. Inhibition of induction of MMPs
is suggested to alleviate photoaging induced by UV irradiation. Zeatin, purified
from Zea mays, is a member of the cytokinin group of plant growth factors, the
activity of which is attributed to its more stable trans form. In this study,
we investigated the effect of trans-Zeatin on UVB-induced matrix metalloproteinase-1
(MMP-1) expression in cultured human skin fibroblasts (HSFs) and studied the mechanisms
of its actions. We found that pretreatment with trans-Zeatin significantly inhibits
UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner.
We also observed that trans-Zeatin inhibits UVB-induced phosphorylation of ERK,
JNK and p38 MAP kinases (MAPKs) dose-dependently. As expected, PD98059, an ERK
inhibitor, SP600125, a JNK inhibitor and SB203580, a p38 MAPK inhibitor effectively
inhibit UVB-induced phosphorylation of ERK, JNK and p38 MAPKs, respectively. Moreover,
the inhibitory mechanism of trans-Zeatin was further demonstrated in MMP-1 secretion
using MAPK-specific inhibitors. PD98059, SP600125 and SB203580 suppressed UVB-induced
MMP-1 secretion, which is consistent with the above results. Collectively, our
results suggest that trans-Zeatin inhibits UVB-induced MMP-1 expression, which
may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways in
HSFs. Trans-Zeatin is a potential agent for the management of skin photoaging</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>19288033</pmid><doi>10.3892/ijmm_00000164</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Anthracenes - pharmacology Blotting, Western Cell Survival - drug effects Cell Survival - radiation effects Cells, Cultured Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Enzyme-Linked Immunosorbent Assay Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - radiation effects Flavonoids - pharmacology Humans Imidazoles - pharmacology Infant, Newborn MAP Kinase Signaling System - drug effects MAP Kinase Signaling System - radiation effects Matrix Metalloproteinase 1 - metabolism Mitogen-Activated Protein Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinases - metabolism Plant Growth Regulators - pharmacology Pyridines - pharmacology Skin - cytology Ultraviolet Rays Zeatin - chemistry Zeatin - pharmacology |
| title | Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts |
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