Determinants of sensitivity and specificity in spotted DNA microarrays with unmodified oligonucleotides

DNA microarrays with unmodified oligonucleotides are a cost-effective alternative to cDNA microarrays. This study examined how purity, length, homology and GC content of the oligonucleotide probes influence the sensitivity and specificity of the method using cyanobacterial genes. Oligonucleotide pur...

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Veröffentlicht in:	Genes & Genetic Systems 2004, Vol.79(4), pp.189-197
Hauptverfasser:	Kucho, K. (Nagoya Univ. (Japan)), Yoneda, H, Harada, M, Ishiura, M
Format:	Artikel
Sprache:	eng
Schlagworte:	BACTERIA Base Sequence cost-effective cross-hybridization DNA Genome, Bacterial GENOMES microarray MOLECULAR HYBRIDIZATION Nucleic Acid Hybridization NUCLEOTIDE SEQUENCE NUCLEOTIDES Oligonucleotide Array Sequence Analysis Oligonucleotides - chemistry RNA RNA, Bacterial - isolation & purification Sensitivity and Specificity SYNECHOCYSTIS Synechocystis - genetics unmodified oligonucleotide
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