Coupling Thin-Layer Chromatography with Vibrational Cooling Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry for the Analysis of Ganglioside Mixtures
Thin-layer chromatography (TLC), which is widely used for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest, can be readily coupled to matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometers, but this arrange...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2004-11, Vol.76 (21), p.6484-6491 |
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| creator | Ivleva, Vera B Elkin, Yuri N Budnik, Bogdan A Moyer, Susanne C O'Connor, Peter B Costello, Catherine E |
| description | Thin-layer chromatography (TLC), which is widely used for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest, can be readily coupled to matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometers, but this arrangement usually compromises mass spectral resolution due to the irregularity of the TLC surface. However, TLC can be coupled to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as α-cyano-4-hydroxycinnamic acid is possible. In this work, whole brain gangliosides are separated using TLC; the TLC plates are attached directly to the MALDI target, where the gangliosides are desorbed, ionized, and detected in the FTMS with >70 000 resolving power. |
| doi_str_mv | 10.1021/ac0491556 |
| format | Article |
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However, TLC can be coupled to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as α-cyano-4-hydroxycinnamic acid is possible. 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Chem</addtitle><description>Thin-layer chromatography (TLC), which is widely used for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest, can be readily coupled to matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometers, but this arrangement usually compromises mass spectral resolution due to the irregularity of the TLC surface. However, TLC can be coupled to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as α-cyano-4-hydroxycinnamic acid is possible. 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Chem</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>76</volume><issue>21</issue><spage>6484</spage><epage>6491</epage><pages>6484-6491</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Thin-layer chromatography (TLC), which is widely used for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest, can be readily coupled to matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometers, but this arrangement usually compromises mass spectral resolution due to the irregularity of the TLC surface. However, TLC can be coupled to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as α-cyano-4-hydroxycinnamic acid is possible. In this work, whole brain gangliosides are separated using TLC; the TLC plates are attached directly to the MALDI target, where the gangliosides are desorbed, ionized, and detected in the FTMS with >70 000 resolving power.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15516145</pmid><doi>10.1021/ac0491556</doi><tpages>8</tpages></addata></record> |
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| subjects | Analytical chemistry Carbohydrate Sequence Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Thin Layer - methods Exact sciences and technology Fourier Analysis Gangliosides - analysis Gangliosides - chemistry Mass Spectrometry - methods Molecular Sequence Data Other chromatographic methods Reference Standards Spectrometric and optical methods |
| title | Coupling Thin-Layer Chromatography with Vibrational Cooling Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry for the Analysis of Ganglioside Mixtures |
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