Synaptobrevin is essential for fast synaptic-vesicle endocytosis
Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle...
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| Veröffentlicht in: | Nature cell biology 2004-11, Vol.6 (11), p.1102-1108 |
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| creator | Südhof, Thomas C Kavalali, Ege T Deák, Ferenc Schoch, Susanne Liu, Xinran |
| description | Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles. |
| doi_str_mv | 10.1038/ncb1185 |
| format | Article |
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Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles.</description><identifier>ISSN: 1465-7392</identifier><identifier>ISSN: 1476-4679</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb1185</identifier><identifier>PMID: 15475946</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biomedical and Life Sciences ; Calcium ; Cancer Research ; Cell Biology ; Developmental Biology ; Endocytosis ; Endocytosis - physiology ; Horseradish Peroxidase - metabolism ; In Vitro Techniques ; letter ; Life Sciences ; Membrane proteins ; Membrane Proteins - genetics ; Membrane Proteins - physiology ; Mice ; Mice, Knockout ; Microscopy, Electron ; Physiological aspects ; Pyridinium Compounds - metabolism ; Quaternary Ammonium Compounds - metabolism ; R-SNARE Proteins ; Stem Cells ; Synapses ; Synaptic Vesicles - metabolism ; Synaptic Vesicles - physiology</subject><ispartof>Nature cell biology, 2004-11, Vol.6 (11), p.1102-1108</ispartof><rights>Springer Nature Limited 2004</rights><rights>COPYRIGHT 2004 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-776c6e79b326c35ef9a26dc0e6f44e4a1e0c33179ae9d72788ee91ff9f3941883</citedby><cites>FETCH-LOGICAL-c529t-776c6e79b326c35ef9a26dc0e6f44e4a1e0c33179ae9d72788ee91ff9f3941883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ncb1185$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ncb1185$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2727,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15475946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Südhof, Thomas C</creatorcontrib><creatorcontrib>Kavalali, Ege T</creatorcontrib><creatorcontrib>Deák, Ferenc</creatorcontrib><creatorcontrib>Schoch, Susanne</creatorcontrib><creatorcontrib>Liu, Xinran</creatorcontrib><title>Synaptobrevin is essential for fast synaptic-vesicle endocytosis</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Endocytosis</subject><subject>Endocytosis - physiology</subject><subject>Horseradish Peroxidase - metabolism</subject><subject>In Vitro Techniques</subject><subject>letter</subject><subject>Life Sciences</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - physiology</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Microscopy, Electron</subject><subject>Physiological aspects</subject><subject>Pyridinium Compounds - metabolism</subject><subject>Quaternary Ammonium Compounds - metabolism</subject><subject>R-SNARE Proteins</subject><subject>Stem Cells</subject><subject>Synapses</subject><subject>Synaptic Vesicles - metabolism</subject><subject>Synaptic Vesicles - physiology</subject><issn>1465-7392</issn><issn>1476-4679</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0VtrFDEUB_BBLLZW8RMog4LVh6m5TS5vLcVLoSBYfQ7ZzMmSMpusSaZ0v71ZZ7WsCpKHhJzf-cPhNM0zjE4xovJdsAuMZf-gOcJM8I5xoR5u37zvBFXksHmc8w1CmDEkHjWHuGeiV4wfNWfXm2DWJS4S3PrQ-txCzhCKN2PrYmqdyaXNP4233S1kb0doIQzRbkrMPj9pDpwZMzzd3cfNtw_vv1586q4-f7y8OL_qbE9U6YTgloNQC0q4pT04ZQgfLALuGANmMCBLKRbKgBoEEVICKOycclQxLCU9bl7PuesUv0-Qi175bGEcTYA4Zc0FIn3f_x9iISRiSFX48g94E6cU6hCaEEK54JhV9GpGSzOC9sHFkozdJupzLCmRkiBc1ek_VD0DrLyNAZyv_3sNb_caqilwV5ZmyllfXn_ZtyeztSnmnMDpdfIrkzYaI73dvt5tv8oXu4mmxQqGe7dbdwVvZpBrKSwh3Y_8d9bzmQZTpgS_s37VfwDavb6N</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Südhof, Thomas C</creator><creator>Kavalali, Ege T</creator><creator>Deák, Ferenc</creator><creator>Schoch, Susanne</creator><creator>Liu, Xinran</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Synaptobrevin is essential for fast synaptic-vesicle endocytosis</title><author>Südhof, Thomas C ; Kavalali, Ege T ; Deák, Ferenc ; Schoch, Susanne ; Liu, Xinran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-776c6e79b326c35ef9a26dc0e6f44e4a1e0c33179ae9d72788ee91ff9f3941883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium</topic><topic>Cancer Research</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>Endocytosis</topic><topic>Endocytosis - 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Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Südhof, Thomas C</au><au>Kavalali, Ege T</au><au>Deák, Ferenc</au><au>Schoch, Susanne</au><au>Liu, Xinran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synaptobrevin is essential for fast synaptic-vesicle endocytosis</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>6</volume><issue>11</issue><spage>1102</spage><epage>1108</epage><pages>1102-1108</pages><issn>1465-7392</issn><issn>1476-4679</issn><eissn>1476-4679</eissn><abstract>Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>15475946</pmid><doi>10.1038/ncb1185</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biomedical and Life Sciences Calcium Cancer Research Cell Biology Developmental Biology Endocytosis Endocytosis - physiology Horseradish Peroxidase - metabolism In Vitro Techniques letter Life Sciences Membrane proteins Membrane Proteins - genetics Membrane Proteins - physiology Mice Mice, Knockout Microscopy, Electron Physiological aspects Pyridinium Compounds - metabolism Quaternary Ammonium Compounds - metabolism R-SNARE Proteins Stem Cells Synapses Synaptic Vesicles - metabolism Synaptic Vesicles - physiology |
| title | Synaptobrevin is essential for fast synaptic-vesicle endocytosis |
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