Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting
The study describes the first attempt to record leptospirosis in Erode by isolation and serological tests such as the microscopic agglutination test and IgM-based enzyme-linked immunosorbent assay. Twenty-nine clinically suspected cases showing fever, headache, body ache associated with jaundice, de...
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| Veröffentlicht in: | Japanese journal of infectious diseases 2004-10, Vol.57 (5), p.193-197 |
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| container_title | Japanese journal of infectious diseases |
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| creator | Natarajaseenivasan, Kalimuthusamy Prabhu, Nagarajan Selvanayaki, Krishnaswamy Raja, Suresh Savalaikarankulam Sudalaimuthu Ratnam, Sivalingam |
| description | The study describes the first attempt to record leptospirosis in Erode by isolation and serological tests such as the microscopic agglutination test and IgM-based enzyme-linked immunosorbent assay. Twenty-nine clinically suspected cases showing fever, headache, body ache associated with jaundice, decreased urine output, and conjunctival suffusion were included. The age of the patients ranged between 10-71 years and most of them were agricultural workers. Paired sera were possible among 12 cases. All the patients had fever and headache and other more common symptoms were myalgia and icterus. Leptospiral culture was positive in 7 (24.1%) patients. Out of 29 patients, 26 (89.7%) were diagnosed as having current leptospiral infection based on serology and isolation. The leptospiral isolates KSR 1-6 were further characterized by using randomly amplified polymorphic DNA fingerprinting shown genetic similarities with Leptospira interrogans spp. This study shows the presence of leptospirosis among the hospital cases of Erode and that this disease is a potential health hazard of agricultural workers in Cauvery basin. |
| format | Article |
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The leptospiral isolates KSR 1-6 were further characterized by using randomly amplified polymorphic DNA fingerprinting shown genetic similarities with Leptospira interrogans spp. This study shows the presence of leptospirosis among the hospital cases of Erode and that this disease is a potential health hazard of agricultural workers in Cauvery basin.</description><identifier>ISSN: 1344-6304</identifier><identifier>PMID: 15507774</identifier><language>eng</language><publisher>Japan</publisher><subject>Adolescent ; Adult ; Aged ; Child ; DNA Fingerprinting - methods ; Female ; Humans ; India - epidemiology ; Leptospira - genetics ; Leptospira - isolation & purification ; Leptospirosis - epidemiology ; Leptospirosis - microbiology ; Male ; Middle Aged ; Phylogeny ; Random Amplified Polymorphic DNA Technique ; Seroepidemiologic Studies</subject><ispartof>Japanese journal of infectious diseases, 2004-10, Vol.57 (5), p.193-197</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15507774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Natarajaseenivasan, Kalimuthusamy</creatorcontrib><creatorcontrib>Prabhu, Nagarajan</creatorcontrib><creatorcontrib>Selvanayaki, Krishnaswamy</creatorcontrib><creatorcontrib>Raja, Suresh Savalaikarankulam Sudalaimuthu</creatorcontrib><creatorcontrib>Ratnam, Sivalingam</creatorcontrib><title>Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting</title><title>Japanese journal of infectious diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>The study describes the first attempt to record leptospirosis in Erode by isolation and serological tests such as the microscopic agglutination test and IgM-based enzyme-linked immunosorbent assay. Twenty-nine clinically suspected cases showing fever, headache, body ache associated with jaundice, decreased urine output, and conjunctival suffusion were included. The age of the patients ranged between 10-71 years and most of them were agricultural workers. Paired sera were possible among 12 cases. All the patients had fever and headache and other more common symptoms were myalgia and icterus. Leptospiral culture was positive in 7 (24.1%) patients. Out of 29 patients, 26 (89.7%) were diagnosed as having current leptospiral infection based on serology and isolation. The leptospiral isolates KSR 1-6 were further characterized by using randomly amplified polymorphic DNA fingerprinting shown genetic similarities with Leptospira interrogans spp. This study shows the presence of leptospirosis among the hospital cases of Erode and that this disease is a potential health hazard of agricultural workers in Cauvery basin.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Child</subject><subject>DNA Fingerprinting - methods</subject><subject>Female</subject><subject>Humans</subject><subject>India - epidemiology</subject><subject>Leptospira - genetics</subject><subject>Leptospira - isolation & purification</subject><subject>Leptospirosis - epidemiology</subject><subject>Leptospirosis - microbiology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phylogeny</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Seroepidemiologic Studies</subject><issn>1344-6304</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kN1KwzAcxXuhuDl9BflficIKSZs0q3djTjcYKn5clzRJ14y0qUl6UZ_GR7XovDqHw4_D4ZxEU5wSEmcpIpPo3PsDQgmlGJ1FE0wpYoyRafS96RveglFdsL7TznrtQbewdlaqObzZPtSwbaXmd-CVs8buhzlobw0P2rZz4K0EUXPHRVBOf_2mYCsItTpiykM5gBtB25gBeNMZXWklobNmaKzrai3g_mkJN6_Ll_tbqHS7V65zug2ju4hOK268ujzqLPp4WL-vNvHu-XG7Wu7iQ0KzEGNOhZSMM6mSqiwZIZjlCAkm8hzRnFaLjOQiUXkqEkpymSwow3JRyRyXKKNlOouu_3o7Zz975UPRaC-UMbxVtvdFxhDOCMMjeHUE-7JRshiHNtwNxf-n6Q83zHRu</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Natarajaseenivasan, Kalimuthusamy</creator><creator>Prabhu, Nagarajan</creator><creator>Selvanayaki, Krishnaswamy</creator><creator>Raja, Suresh Savalaikarankulam Sudalaimuthu</creator><creator>Ratnam, Sivalingam</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting</title><author>Natarajaseenivasan, Kalimuthusamy ; Prabhu, Nagarajan ; Selvanayaki, Krishnaswamy ; Raja, Suresh Savalaikarankulam Sudalaimuthu ; Ratnam, Sivalingam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j256t-1a5cdd7a7de2fbb74417900c7c990595f8649c2e93c2549d28571d8fd91b065b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Child</topic><topic>DNA Fingerprinting - methods</topic><topic>Female</topic><topic>Humans</topic><topic>India - epidemiology</topic><topic>Leptospira - genetics</topic><topic>Leptospira - isolation & purification</topic><topic>Leptospirosis - epidemiology</topic><topic>Leptospirosis - microbiology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phylogeny</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Seroepidemiologic Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Natarajaseenivasan, Kalimuthusamy</creatorcontrib><creatorcontrib>Prabhu, Nagarajan</creatorcontrib><creatorcontrib>Selvanayaki, Krishnaswamy</creatorcontrib><creatorcontrib>Raja, Suresh Savalaikarankulam Sudalaimuthu</creatorcontrib><creatorcontrib>Ratnam, Sivalingam</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Natarajaseenivasan, Kalimuthusamy</au><au>Prabhu, Nagarajan</au><au>Selvanayaki, Krishnaswamy</au><au>Raja, Suresh Savalaikarankulam Sudalaimuthu</au><au>Ratnam, Sivalingam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting</atitle><jtitle>Japanese journal of infectious diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>57</volume><issue>5</issue><spage>193</spage><epage>197</epage><pages>193-197</pages><issn>1344-6304</issn><abstract>The study describes the first attempt to record leptospirosis in Erode by isolation and serological tests such as the microscopic agglutination test and IgM-based enzyme-linked immunosorbent assay. 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The leptospiral isolates KSR 1-6 were further characterized by using randomly amplified polymorphic DNA fingerprinting shown genetic similarities with Leptospira interrogans spp. This study shows the presence of leptospirosis among the hospital cases of Erode and that this disease is a potential health hazard of agricultural workers in Cauvery basin.</abstract><cop>Japan</cop><pmid>15507774</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Japanese journal of infectious diseases, 2004-10, Vol.57 (5), p.193-197 |
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| language | eng |
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| source | Freely Accessible Japanese Titles - check A-Z of ejournals; MEDLINE; J-STAGE フリー; EZB Electronic Journals Library |
| subjects | Adolescent Adult Aged Child DNA Fingerprinting - methods Female Humans India - epidemiology Leptospira - genetics Leptospira - isolation & purification Leptospirosis - epidemiology Leptospirosis - microbiology Male Middle Aged Phylogeny Random Amplified Polymorphic DNA Technique Seroepidemiologic Studies |
| title | Human leptospirosis in Erode, South India: serology, isolation, and characterization of the isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting |
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