Vr2: a new apple scab resistance gene

Reports from several European countries of the breakdown of the Vf resistance, the most frequently used source of resistance in breeding programs against apple scab, emphasize the urgency of diversifying the basis of apple scab resistance and pyramiding different apple scab resistances with the use...

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Veröffentlicht in:Theoretical and applied genetics 2004-09, Vol.109 (5), p.1087-1092
Hauptverfasser: PATOCCHI, A, BIGLER, B, KOLLER, B, KELLERHALS, M, GESSLER, C
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container_issue 5
container_start_page 1087
container_title Theoretical and applied genetics
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creator PATOCCHI, A
BIGLER, B
KOLLER, B
KELLERHALS, M
GESSLER, C
description Reports from several European countries of the breakdown of the Vf resistance, the most frequently used source of resistance in breeding programs against apple scab, emphasize the urgency of diversifying the basis of apple scab resistance and pyramiding different apple scab resistances with the use of their associated molecular markers. GMAL 2473 is an apple scab resistant selection thought to carry the resistance gene Vr. We report the identification by BSA of three AFLP markers and one RAPD marker associated with the GMAL 2473 resistance gene. SSRs associated with the resistance gene were found by (1) identifying the linkage group carrying the apple scab resistance and (2) testing the SSRs previously mapped in the same region. One such SSR, CH02c02a, mapped on linkage group 2, co-segregates with the resistance gene. GAML 2473 was tested with molecular markers associated with other apple scab resistance genes, and accessions carrying known apple scab resistance genes were tested with the SSR linked to the resistance gene found in GMAL 2473. The results indicate that GMAL 2473 does not carry Vr, and that a new apple scab resistance gene, named Vr2, has been identified.
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GMAL 2473 is an apple scab resistant selection thought to carry the resistance gene Vr. We report the identification by BSA of three AFLP markers and one RAPD marker associated with the GMAL 2473 resistance gene. SSRs associated with the resistance gene were found by (1) identifying the linkage group carrying the apple scab resistance and (2) testing the SSRs previously mapped in the same region. One such SSR, CH02c02a, mapped on linkage group 2, co-segregates with the resistance gene. GAML 2473 was tested with molecular markers associated with other apple scab resistance genes, and accessions carrying known apple scab resistance genes were tested with the SSR linked to the resistance gene found in GMAL 2473. 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subjects Ascomycota
Biological and medical sciences
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Crop diseases
Crosses, Genetic
Fundamental and applied biological sciences. Psychology
Genes, Plant - genetics
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genomics
Immunity, Innate - genetics
Malus - genetics
Minisatellite Repeats - genetics
Nucleic Acid Amplification Techniques
Plant Diseases - genetics
Plant Diseases - microbiology
Polymorphism, Restriction Fragment Length
Pteridophyta, spermatophyta
Random Amplified Polymorphic DNA Technique
Vegetals
title Vr2: a new apple scab resistance gene
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