Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation
We isolated 11 independent temperature-sensitive (ts) mutants of Schizosaccharomyces pombe RanGAP, SpRna1 that have several amino acid changes in the conserved domains of RanGAP. Resulting Sprna1ts showed a strong defect in mitotic chromosome segregation, but did not in nucleocytoplasmic transport a...
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| Veröffentlicht in: | Molecular biology of the cell 2004-11, Vol.15 (11), p.4960-4970 |
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| creator | Kusano, Ayumi Yoshioka, Tomoko Nishijima, Hitoshi Nishitani, Hideo Nishimoto, Takeharu |
| description | We isolated 11 independent temperature-sensitive (ts) mutants of Schizosaccharomyces pombe RanGAP, SpRna1 that have several amino acid changes in the conserved domains of RanGAP. Resulting Sprna1ts showed a strong defect in mitotic chromosome segregation, but did not in nucleocytoplasmic transport and microtubule formation. In addition to Sprna1+ and Spksp1+, the clr4+ (histone H3-K9 methyltransferase), the S. pombe gene, SPAC25A8.01c, designated snf2SR+ (a member of the chromatin remodeling factors, Snf2 family with DNA-dependent ATPase activity), but not the spi1+ (S. pombe Ran homolog), rescued a lethality of Sprna1ts. Both Clr4 and Snf2 were reported to be involved in heterochromatin formation essential for building the centromeres. Consistently, Sprna1ts was defective in gene-silencing at the centromeres. But a silencing at the telomere, another heterochromatic region, was normal in all of Sprna1ts strains, indicating SpRna1 in general did not function for a heterochromatin formation. snf2SR+ rescued a centromeric silencing defect and Deltaclr4+ was synthetic lethal with Sprna1ts. Taken together, SpRna1 was suggested to function for constructing the centromeres, by cooperating with Clr4 and Snf2SR. Loss of SpRna1 activity, therefore, caused chromosome missegregation. |
| doi_str_mv | 10.1091/mbc.E04-01-0067 |
| format | Article |
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Resulting Sprna1ts showed a strong defect in mitotic chromosome segregation, but did not in nucleocytoplasmic transport and microtubule formation. In addition to Sprna1+ and Spksp1+, the clr4+ (histone H3-K9 methyltransferase), the S. pombe gene, SPAC25A8.01c, designated snf2SR+ (a member of the chromatin remodeling factors, Snf2 family with DNA-dependent ATPase activity), but not the spi1+ (S. pombe Ran homolog), rescued a lethality of Sprna1ts. Both Clr4 and Snf2 were reported to be involved in heterochromatin formation essential for building the centromeres. Consistently, Sprna1ts was defective in gene-silencing at the centromeres. But a silencing at the telomere, another heterochromatic region, was normal in all of Sprna1ts strains, indicating SpRna1 in general did not function for a heterochromatin formation. snf2SR+ rescued a centromeric silencing defect and Deltaclr4+ was synthetic lethal with Sprna1ts. Taken together, SpRna1 was suggested to function for constructing the centromeres, by cooperating with Clr4 and Snf2SR. Loss of SpRna1 activity, therefore, caused chromosome missegregation.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.E04-01-0067</identifier><identifier>PMID: 15317843</identifier><language>eng</language><publisher>United States</publisher><subject>Active Transport, Cell Nucleus ; Cell Nucleus - metabolism ; Centromere - ultrastructure ; Chromosome Segregation ; Cytoplasm - metabolism ; DNA Mutational Analysis ; Gene Silencing ; Green Fluorescent Proteins - metabolism ; GTPase-Activating Proteins - biosynthesis ; GTPase-Activating Proteins - physiology ; Heterochromatin - metabolism ; Histones ; Microscopy, Fluorescence ; Mitosis ; Models, Biological ; Models, Molecular ; Mutation ; Protein Structure, Tertiary ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe Proteins ; Temperature</subject><ispartof>Molecular biology of the cell, 2004-11, Vol.15 (11), p.4960-4970</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-79fcecf34548092684d42154615a6a89808285a8135a6dc8a4e72b5c1b069c613</citedby><cites>FETCH-LOGICAL-c412t-79fcecf34548092684d42154615a6a89808285a8135a6dc8a4e72b5c1b069c613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15317843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kusano, Ayumi</creatorcontrib><creatorcontrib>Yoshioka, Tomoko</creatorcontrib><creatorcontrib>Nishijima, Hitoshi</creatorcontrib><creatorcontrib>Nishitani, Hideo</creatorcontrib><creatorcontrib>Nishimoto, Takeharu</creatorcontrib><title>Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>We isolated 11 independent temperature-sensitive (ts) mutants of Schizosaccharomyces pombe RanGAP, SpRna1 that have several amino acid changes in the conserved domains of RanGAP. Resulting Sprna1ts showed a strong defect in mitotic chromosome segregation, but did not in nucleocytoplasmic transport and microtubule formation. In addition to Sprna1+ and Spksp1+, the clr4+ (histone H3-K9 methyltransferase), the S. pombe gene, SPAC25A8.01c, designated snf2SR+ (a member of the chromatin remodeling factors, Snf2 family with DNA-dependent ATPase activity), but not the spi1+ (S. pombe Ran homolog), rescued a lethality of Sprna1ts. Both Clr4 and Snf2 were reported to be involved in heterochromatin formation essential for building the centromeres. Consistently, Sprna1ts was defective in gene-silencing at the centromeres. But a silencing at the telomere, another heterochromatic region, was normal in all of Sprna1ts strains, indicating SpRna1 in general did not function for a heterochromatin formation. snf2SR+ rescued a centromeric silencing defect and Deltaclr4+ was synthetic lethal with Sprna1ts. Taken together, SpRna1 was suggested to function for constructing the centromeres, by cooperating with Clr4 and Snf2SR. Loss of SpRna1 activity, therefore, caused chromosome missegregation.</description><subject>Active Transport, Cell Nucleus</subject><subject>Cell Nucleus - metabolism</subject><subject>Centromere - ultrastructure</subject><subject>Chromosome Segregation</subject><subject>Cytoplasm - metabolism</subject><subject>DNA Mutational Analysis</subject><subject>Gene Silencing</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>GTPase-Activating Proteins - biosynthesis</subject><subject>GTPase-Activating Proteins - physiology</subject><subject>Heterochromatin - metabolism</subject><subject>Histones</subject><subject>Microscopy, Fluorescence</subject><subject>Mitosis</subject><subject>Models, Biological</subject><subject>Models, Molecular</subject><subject>Mutation</subject><subject>Protein Structure, Tertiary</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>Temperature</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkL1v3DAMxYWiRfPRzt0KTZ3OCWlLsjQGQZoGOCBF0s6CTNN3KmzrIt0N6V8fBzkgE8nH997wE-IbwgWCw8upo4sbUBVgBWDaD-IUXeMqpa35uOygXYW6VifirJR_AKiUaT-LE9QNtlY1pyI_0jb-TyUQbUNO0zNxkbs0dSwfwnx79Vtu05TGtFnJx93DHHAlY5GZnw4xcy-HlCXxvF-SnCPJEkeeKc4bGeZe0nbRU1l-svAm8ybsY5q_iE9DGAt_Pc5z8ffnzZ_rX9X6_vbu-mpdkcJ6X7VuIKahUVpZcLWxqlc1amVQBxOss2Brq4PFZrl7skFxW3easAPjyGBzLn689e5yejpw2fspFuJxDDOnQ_GmBXBtaxfj5ZuRciol8-B3OU4hP3sE_4rZL5g9g_KA_hXzkvh-rD50E_fv_iPX5gULTHmp</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Kusano, Ayumi</creator><creator>Yoshioka, Tomoko</creator><creator>Nishijima, Hitoshi</creator><creator>Nishitani, Hideo</creator><creator>Nishimoto, Takeharu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200411</creationdate><title>Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation</title><author>Kusano, Ayumi ; Yoshioka, Tomoko ; Nishijima, Hitoshi ; Nishitani, Hideo ; Nishimoto, Takeharu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-79fcecf34548092684d42154615a6a89808285a8135a6dc8a4e72b5c1b069c613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Cell Nucleus - metabolism</topic><topic>Centromere - ultrastructure</topic><topic>Chromosome Segregation</topic><topic>Cytoplasm - metabolism</topic><topic>DNA Mutational Analysis</topic><topic>Gene Silencing</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>GTPase-Activating Proteins - biosynthesis</topic><topic>GTPase-Activating Proteins - physiology</topic><topic>Heterochromatin - metabolism</topic><topic>Histones</topic><topic>Microscopy, Fluorescence</topic><topic>Mitosis</topic><topic>Models, Biological</topic><topic>Models, Molecular</topic><topic>Mutation</topic><topic>Protein Structure, Tertiary</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kusano, Ayumi</creatorcontrib><creatorcontrib>Yoshioka, Tomoko</creatorcontrib><creatorcontrib>Nishijima, Hitoshi</creatorcontrib><creatorcontrib>Nishitani, Hideo</creatorcontrib><creatorcontrib>Nishimoto, Takeharu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kusano, Ayumi</au><au>Yoshioka, Tomoko</au><au>Nishijima, Hitoshi</au><au>Nishitani, Hideo</au><au>Nishimoto, Takeharu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2004-11</date><risdate>2004</risdate><volume>15</volume><issue>11</issue><spage>4960</spage><epage>4970</epage><pages>4960-4970</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><abstract>We isolated 11 independent temperature-sensitive (ts) mutants of Schizosaccharomyces pombe RanGAP, SpRna1 that have several amino acid changes in the conserved domains of RanGAP. Resulting Sprna1ts showed a strong defect in mitotic chromosome segregation, but did not in nucleocytoplasmic transport and microtubule formation. In addition to Sprna1+ and Spksp1+, the clr4+ (histone H3-K9 methyltransferase), the S. pombe gene, SPAC25A8.01c, designated snf2SR+ (a member of the chromatin remodeling factors, Snf2 family with DNA-dependent ATPase activity), but not the spi1+ (S. pombe Ran homolog), rescued a lethality of Sprna1ts. Both Clr4 and Snf2 were reported to be involved in heterochromatin formation essential for building the centromeres. Consistently, Sprna1ts was defective in gene-silencing at the centromeres. But a silencing at the telomere, another heterochromatic region, was normal in all of Sprna1ts strains, indicating SpRna1 in general did not function for a heterochromatin formation. snf2SR+ rescued a centromeric silencing defect and Deltaclr4+ was synthetic lethal with Sprna1ts. 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| subjects | Active Transport, Cell Nucleus Cell Nucleus - metabolism Centromere - ultrastructure Chromosome Segregation Cytoplasm - metabolism DNA Mutational Analysis Gene Silencing Green Fluorescent Proteins - metabolism GTPase-Activating Proteins - biosynthesis GTPase-Activating Proteins - physiology Heterochromatin - metabolism Histones Microscopy, Fluorescence Mitosis Models, Biological Models, Molecular Mutation Protein Structure, Tertiary Schizosaccharomyces - metabolism Schizosaccharomyces pombe Proteins Temperature |
| title | Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation |
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