What is your diagnosis? Lymph node cytology from a dog

: A 2‐year‐old, castrated male, mixed‐breed dog was presented to the University of Florida Veterinary Medical Center with swelling, edema, ulceration, and draining tracts in the region surrounding the left hock. The dog had mild monocytosis and moderate hyperglobulinemia. Fine‐needle aspirate specim...

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Veröffentlicht in:Veterinary clinical pathology 2009-03, Vol.38 (1), p.91-93
Hauptverfasser: Dunbar, Mark D, Wamsley, Heather L
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description : A 2‐year‐old, castrated male, mixed‐breed dog was presented to the University of Florida Veterinary Medical Center with swelling, edema, ulceration, and draining tracts in the region surrounding the left hock. The dog had mild monocytosis and moderate hyperglobulinemia. Fine‐needle aspirate specimens of the left popliteal lymph node revealed pyogranulomatous lymphadenitis with hyphal organisms. The diameters of the hyphae were variable, ranging from 11 to 22 μm. The organism was considered as most consistent with Lagenidium caninum; although Pythium insidiosum or Lagenidium karlingii were not conclusively excluded, hyphal diameter in these organisms is typically smaller (6.6–8.8 and 2.5–11 μm, respectively). A positive Western blot confirmed the presence of serum antibodies reactive against Lagenidium sp. and the absence of antibodies to P. insidoisum, Basidiobolus, and Conidiobolus antibodies. Careful assessment of hyphal diameter in cytologic specimens may be useful in differentiating L. caninum from P. insidiosum or L. karlingii.
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A positive Western blot confirmed the presence of serum antibodies reactive against Lagenidium sp. and the absence of antibodies to P. insidoisum, Basidiobolus, and Conidiobolus antibodies. 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Lymph node cytology from a dog</title><title>Veterinary clinical pathology</title><addtitle>Vet Clin Pathol</addtitle><description>: A 2‐year‐old, castrated male, mixed‐breed dog was presented to the University of Florida Veterinary Medical Center with swelling, edema, ulceration, and draining tracts in the region surrounding the left hock. The dog had mild monocytosis and moderate hyperglobulinemia. Fine‐needle aspirate specimens of the left popliteal lymph node revealed pyogranulomatous lymphadenitis with hyphal organisms. The diameters of the hyphae were variable, ranging from 11 to 22 μm. The organism was considered as most consistent with Lagenidium caninum; although Pythium insidiosum or Lagenidium karlingii were not conclusively excluded, hyphal diameter in these organisms is typically smaller (6.6–8.8 and 2.5–11 μm, respectively). A positive Western blot confirmed the presence of serum antibodies reactive against Lagenidium sp. and the absence of antibodies to P. insidoisum, Basidiobolus, and Conidiobolus antibodies. 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source MEDLINE; Wiley Online Library Journals
subjects animal pathogenic fungi
Animals
diameter
disease diagnosis
dog diseases
Dog Diseases - microbiology
Dog Diseases - pathology
Dogs
Fungal
hyphae
Infection - veterinary
Lagenidium
Lagenidium - isolation & purification
Lagenidium caninum
Lagenidium karlingii
lymph node cytology
lymph nodes
Lymph Nodes - cytology
lymphadenitis
Male
mycoses
oomycetal infection
pathogen identification
pets
pseudofungal
Pythium
Pythium insidiosum
serodiagnosis
signs and symptoms (animals and humans)
title What is your diagnosis? Lymph node cytology from a dog
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