Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma
We isolated a single-cell-derived cell line from a spinal hamartoma, a which occurred in a newborn boy and was associated with a rudimentary limb. The maternal cells (HHC-7) differentiated into osteoblasts, chondrocytes, adipocytes, and skeletal muscles when they were cultured in differentiation-ind...
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| Veröffentlicht in: | Cell and tissue research 2004-09, Vol.317 (3), p.237-246 |
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| creator | Doiguchi, Y Tsukazaki, T Tomonaga, T Nobuta, M Fujita, S Hayashi, T Nagai, K Matsumoto, T Shindo, H Yamaguchi, A |
| description | We isolated a single-cell-derived cell line from a spinal hamartoma, a which occurred in a newborn boy and was associated with a rudimentary limb. The maternal cells (HHC-7) differentiated into osteoblasts, chondrocytes, adipocytes, and skeletal muscles when they were cultured in differentiation-inducing media specific to each mesenchymal cell. We isolated a single-cell-derived clonal cell line (Clone K) after transfection with SV40 T antigen. These cells expressed CD73 and CD117, while being negative for expression of CD45. Clone K cells cultured in an osteogenic differentiation medium increased ALP activity and expressed mRNAs for Runx2 and osteocalcin. Treatment with rhBMP-2 induced Clone K cells to differentiate into both osteoblasts and chondrocytes. These cells expressed mRNAs for Sox9 and aggrecan in addition to osteogenic markers. Culture in an adipogenic differentiation medium induced Clone K cells to differentiation into adipocytes, which expressed mRNAs for PPARgamma2 and a2P. Clone K cells cultured in a serum-depleted medium generated desmin-positive cells and expressed MyoD1 mRNA. Clone K cells exhibited numerous alpha-smooth muscle actin-positive cells; however, treatment with rhBMP-2 decreased their number. Clone K cells, transplanted with a carrier containing rhBMP-2 into the muscles of SCID mice, generated ectopic endochondral bone formation. In these tissues, several osteoblasts and chondrocytes expressed SV40 T antigen, indicating their Clone K cell origin. Thus, Clone K cells are useful tools for analyzing the characteristics of human multipotential mesenchymal progenitors. |
| doi_str_mv | 10.1007/s00441-004-0942-2 |
| format | Article |
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The maternal cells (HHC-7) differentiated into osteoblasts, chondrocytes, adipocytes, and skeletal muscles when they were cultured in differentiation-inducing media specific to each mesenchymal cell. We isolated a single-cell-derived clonal cell line (Clone K) after transfection with SV40 T antigen. These cells expressed CD73 and CD117, while being negative for expression of CD45. Clone K cells cultured in an osteogenic differentiation medium increased ALP activity and expressed mRNAs for Runx2 and osteocalcin. Treatment with rhBMP-2 induced Clone K cells to differentiate into both osteoblasts and chondrocytes. These cells expressed mRNAs for Sox9 and aggrecan in addition to osteogenic markers. Culture in an adipogenic differentiation medium induced Clone K cells to differentiation into adipocytes, which expressed mRNAs for PPARgamma2 and a2P. Clone K cells cultured in a serum-depleted medium generated desmin-positive cells and expressed MyoD1 mRNA. Clone K cells exhibited numerous alpha-smooth muscle actin-positive cells; however, treatment with rhBMP-2 decreased their number. Clone K cells, transplanted with a carrier containing rhBMP-2 into the muscles of SCID mice, generated ectopic endochondral bone formation. In these tissues, several osteoblasts and chondrocytes expressed SV40 T antigen, indicating their Clone K cell origin. Thus, Clone K cells are useful tools for analyzing the characteristics of human multipotential mesenchymal progenitors.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-004-0942-2</identifier><identifier>PMID: 15300494</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Adipocytes - cytology ; Animals ; Cell Differentiation ; Cell Line ; Chondrocytes - cytology ; Clone Cells ; Hamartoma - metabolism ; Hamartoma - pathology ; Hamartoma - surgery ; Humans ; Infant, Newborn ; Male ; Mesoderm - cytology ; Mesoderm - metabolism ; Mesoderm - transplantation ; Mice ; Mice, SCID ; Muscle, Skeletal - cytology ; Osteoblasts - cytology ; Spinal Diseases - metabolism ; Spinal Diseases - pathology ; Spinal Diseases - surgery ; Transfection</subject><ispartof>Cell and tissue research, 2004-09, Vol.317 (3), p.237-246</ispartof><rights>Copyright Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-eb479b5250ce9533d52775ae191414090ed1c57aded8c1f840b59655db3eebb03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15300494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doiguchi, Y</creatorcontrib><creatorcontrib>Tsukazaki, T</creatorcontrib><creatorcontrib>Tomonaga, T</creatorcontrib><creatorcontrib>Nobuta, M</creatorcontrib><creatorcontrib>Fujita, S</creatorcontrib><creatorcontrib>Hayashi, T</creatorcontrib><creatorcontrib>Nagai, K</creatorcontrib><creatorcontrib>Matsumoto, T</creatorcontrib><creatorcontrib>Shindo, H</creatorcontrib><creatorcontrib>Yamaguchi, A</creatorcontrib><title>Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>We isolated a single-cell-derived cell line from a spinal hamartoma, a which occurred in a newborn boy and was associated with a rudimentary limb. The maternal cells (HHC-7) differentiated into osteoblasts, chondrocytes, adipocytes, and skeletal muscles when they were cultured in differentiation-inducing media specific to each mesenchymal cell. We isolated a single-cell-derived clonal cell line (Clone K) after transfection with SV40 T antigen. These cells expressed CD73 and CD117, while being negative for expression of CD45. Clone K cells cultured in an osteogenic differentiation medium increased ALP activity and expressed mRNAs for Runx2 and osteocalcin. Treatment with rhBMP-2 induced Clone K cells to differentiate into both osteoblasts and chondrocytes. These cells expressed mRNAs for Sox9 and aggrecan in addition to osteogenic markers. Culture in an adipogenic differentiation medium induced Clone K cells to differentiation into adipocytes, which expressed mRNAs for PPARgamma2 and a2P. Clone K cells cultured in a serum-depleted medium generated desmin-positive cells and expressed MyoD1 mRNA. Clone K cells exhibited numerous alpha-smooth muscle actin-positive cells; however, treatment with rhBMP-2 decreased their number. Clone K cells, transplanted with a carrier containing rhBMP-2 into the muscles of SCID mice, generated ectopic endochondral bone formation. In these tissues, several osteoblasts and chondrocytes expressed SV40 T antigen, indicating their Clone K cell origin. Thus, Clone K cells are useful tools for analyzing the characteristics of human multipotential mesenchymal progenitors.</description><subject>Adipocytes - cytology</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Chondrocytes - cytology</subject><subject>Clone Cells</subject><subject>Hamartoma - metabolism</subject><subject>Hamartoma - pathology</subject><subject>Hamartoma - surgery</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - metabolism</subject><subject>Mesoderm - transplantation</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Muscle, Skeletal - cytology</subject><subject>Osteoblasts - cytology</subject><subject>Spinal Diseases - metabolism</subject><subject>Spinal Diseases - pathology</subject><subject>Spinal Diseases - surgery</subject><subject>Transfection</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkU-LFDEQxYMo7uzqB_AiwYO31vztTI6yrK6w4EXBW6hOVztZOp0xSR_m5kfftDMgeKmC4r1fpfIIecPZB86Y-VgYU4p3rXbMKtGJZ2THlRQd25v9c7JjkonO9P3PK3JdyiNjXPW9fUmuuJbNZNWO_LkrFYY5lEPEpdI0UaB-TgvM9LBGWGjEgos_nGKbeJxnOocFaT1ApRkrhKXQuM41bGP4hXQM04S5sQLUkBbq4Qg-1BOdcooNXo7hLxwi5JoivCIvJpgLvr70G_Lj89332_vu4duXr7efHjovhaodDsrYQQvNPFot5aiFMRqQW664YpbhyL02MOK493zaKzZo22s9DhJxGJi8Ie_P3GNOv1cs1cVQtoNgwbQW15v2o9bIJnz3n_Axrbm9uTjBpdn2qibiZ5HPqZSMkzvm0C46Oc7clo07Z-NadVs2TjTP2wt4HSKO_xyXMOQT8AGL9Q</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Doiguchi, Y</creator><creator>Tsukazaki, T</creator><creator>Tomonaga, T</creator><creator>Nobuta, M</creator><creator>Fujita, S</creator><creator>Hayashi, T</creator><creator>Nagai, K</creator><creator>Matsumoto, T</creator><creator>Shindo, H</creator><creator>Yamaguchi, A</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200409</creationdate><title>Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma</title><author>Doiguchi, Y ; Tsukazaki, T ; Tomonaga, T ; Nobuta, M ; Fujita, S ; Hayashi, T ; Nagai, K ; Matsumoto, T ; Shindo, H ; Yamaguchi, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-eb479b5250ce9533d52775ae191414090ed1c57aded8c1f840b59655db3eebb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adipocytes - cytology</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Chondrocytes - cytology</topic><topic>Clone Cells</topic><topic>Hamartoma - metabolism</topic><topic>Hamartoma - pathology</topic><topic>Hamartoma - surgery</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - metabolism</topic><topic>Mesoderm - transplantation</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Muscle, Skeletal - cytology</topic><topic>Osteoblasts - cytology</topic><topic>Spinal Diseases - metabolism</topic><topic>Spinal Diseases - pathology</topic><topic>Spinal Diseases - surgery</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doiguchi, Y</creatorcontrib><creatorcontrib>Tsukazaki, T</creatorcontrib><creatorcontrib>Tomonaga, T</creatorcontrib><creatorcontrib>Nobuta, M</creatorcontrib><creatorcontrib>Fujita, S</creatorcontrib><creatorcontrib>Hayashi, T</creatorcontrib><creatorcontrib>Nagai, K</creatorcontrib><creatorcontrib>Matsumoto, T</creatorcontrib><creatorcontrib>Shindo, H</creatorcontrib><creatorcontrib>Yamaguchi, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doiguchi, Y</au><au>Tsukazaki, T</au><au>Tomonaga, T</au><au>Nobuta, M</au><au>Fujita, S</au><au>Hayashi, T</au><au>Nagai, K</au><au>Matsumoto, T</au><au>Shindo, H</au><au>Yamaguchi, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>2004-09</date><risdate>2004</risdate><volume>317</volume><issue>3</issue><spage>237</spage><epage>246</epage><pages>237-246</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>We isolated a single-cell-derived cell line from a spinal hamartoma, a which occurred in a newborn boy and was associated with a rudimentary limb. The maternal cells (HHC-7) differentiated into osteoblasts, chondrocytes, adipocytes, and skeletal muscles when they were cultured in differentiation-inducing media specific to each mesenchymal cell. We isolated a single-cell-derived clonal cell line (Clone K) after transfection with SV40 T antigen. These cells expressed CD73 and CD117, while being negative for expression of CD45. Clone K cells cultured in an osteogenic differentiation medium increased ALP activity and expressed mRNAs for Runx2 and osteocalcin. Treatment with rhBMP-2 induced Clone K cells to differentiate into both osteoblasts and chondrocytes. These cells expressed mRNAs for Sox9 and aggrecan in addition to osteogenic markers. Culture in an adipogenic differentiation medium induced Clone K cells to differentiation into adipocytes, which expressed mRNAs for PPARgamma2 and a2P. Clone K cells cultured in a serum-depleted medium generated desmin-positive cells and expressed MyoD1 mRNA. Clone K cells exhibited numerous alpha-smooth muscle actin-positive cells; however, treatment with rhBMP-2 decreased their number. Clone K cells, transplanted with a carrier containing rhBMP-2 into the muscles of SCID mice, generated ectopic endochondral bone formation. In these tissues, several osteoblasts and chondrocytes expressed SV40 T antigen, indicating their Clone K cell origin. Thus, Clone K cells are useful tools for analyzing the characteristics of human multipotential mesenchymal progenitors.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>15300494</pmid><doi>10.1007/s00441-004-0942-2</doi><tpages>10</tpages></addata></record> |
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| subjects | Adipocytes - cytology Animals Cell Differentiation Cell Line Chondrocytes - cytology Clone Cells Hamartoma - metabolism Hamartoma - pathology Hamartoma - surgery Humans Infant, Newborn Male Mesoderm - cytology Mesoderm - metabolism Mesoderm - transplantation Mice Mice, SCID Muscle, Skeletal - cytology Osteoblasts - cytology Spinal Diseases - metabolism Spinal Diseases - pathology Spinal Diseases - surgery Transfection |
| title | Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma |
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