Competitive Protein AdsorptionMultilayer Adsorption and Surface Induced Protein Aggregation
In this study, competitive adsorption of albumin and IgG (immunoglobulin G) from human serum solutions and protein mixtures onto polymer surfaces is studied by means of radioactive labeling. By using two different radiolabels (125I and 131I), albumin and IgG adsorption to polymer surfaces is monitor...
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| Veröffentlicht in: | Langmuir 2009-02, Vol.25 (4), p.2081-2089 |
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| creator | Holmberg, Maria Hou, Xiaolin |
| description | In this study, competitive adsorption of albumin and IgG (immunoglobulin G) from human serum solutions and protein mixtures onto polymer surfaces is studied by means of radioactive labeling. By using two different radiolabels (125I and 131I), albumin and IgG adsorption to polymer surfaces is monitored simultaneously and the influence from the presence of other human serum proteins on albumin and IgG adsorption, as well as their mutual influence during adsorption processes, is investigated. Exploring protein adsorption by combining analysis of competitive adsorption from complex solutions of high concentration with investigation of single protein adsorption and interdependent adsorption between two specific proteins enables us to map protein adsorption sequences during competitive protein adsorption. Our study shows that proteins can adsorb in a multilayer fashion onto the polymer surfaces and that the outcome of IgG adsorption is much more sensitive to surface characteristics than the outcome of albumin adsorption. Using high concentrations of protein solution and hydrophobic polymer surfaces during adsorption can induce IgG aggregation, which is observed as extremely high IgG adsorptions. Besides using a more hydrophilic substrate, surface-induced IgG aggregation can be inhibited by changing the adsorption sequence of albumin and IgG. |
| doi_str_mv | 10.1021/la8031978 |
| format | Article |
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Using high concentrations of protein solution and hydrophobic polymer surfaces during adsorption can induce IgG aggregation, which is observed as extremely high IgG adsorptions. 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By using two different radiolabels (125I and 131I), albumin and IgG adsorption to polymer surfaces is monitored simultaneously and the influence from the presence of other human serum proteins on albumin and IgG adsorption, as well as their mutual influence during adsorption processes, is investigated. Exploring protein adsorption by combining analysis of competitive adsorption from complex solutions of high concentration with investigation of single protein adsorption and interdependent adsorption between two specific proteins enables us to map protein adsorption sequences during competitive protein adsorption. Our study shows that proteins can adsorb in a multilayer fashion onto the polymer surfaces and that the outcome of IgG adsorption is much more sensitive to surface characteristics than the outcome of albumin adsorption. Using high concentrations of protein solution and hydrophobic polymer surfaces during adsorption can induce IgG aggregation, which is observed as extremely high IgG adsorptions. Besides using a more hydrophilic substrate, surface-induced IgG aggregation can be inhibited by changing the adsorption sequence of albumin and IgG.</description><subject>Adsorption</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Humans</subject><subject>Immunoglobulin G - chemistry</subject><subject>Immunoglobulin G - ultrastructure</subject><subject>Interfaces: Adsorption, Reactions, Films, Forces</subject><subject>Microscopy, Atomic Force</subject><subject>Serum Albumin - chemistry</subject><subject>Surface physical chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtKw0AUBuBBFFsvC19AslFwEZ0zM7nMshQvhYqCujWcTE5KSprEmUTok_gqPpXPYEpL60JmcWDON__Az9gZ8GvgAm5KjLkEHcV7bAiB4H4Qi2ifDXmkpB-pUA7YkXNzzrmWSh-yAWjQOgI9ZO_jetFQW7TFJ3nPtm6pqLxR5mrbtEVd_Xx9P3ZlW5S4JPvn3sMq8146m6Mhb1JlnaFs93w2szTDlTthBzmWjk4385i93d2-jh_86dP9ZDya-qi4bv08l6k2IcgoQGECoLQ_CGGWqhj6DaSBACSSJtQmJSUw5Jp0JlUEZISSx-xyndvY-qMj1yaLwhkqS6yo7lwSRpyHUskeXq2hsbVzlvKkscUC7TIBnqzKTLZl9vZ8E9qlC8p2ctNeDy42AJ3BMrdYmcJtnQBQXHDYOTQumdedrfou_vnwF40BirU</recordid><startdate>20090217</startdate><enddate>20090217</enddate><creator>Holmberg, Maria</creator><creator>Hou, Xiaolin</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090217</creationdate><title>Competitive Protein AdsorptionMultilayer Adsorption and Surface Induced Protein Aggregation</title><author>Holmberg, Maria ; Hou, Xiaolin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-ff3b9c61375a2c51ebebea16db481b9c1b521aee3c69cbe42a609e9d3471ec243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adsorption</topic><topic>Chemistry</topic><topic>Colloidal state and disperse state</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Humans</topic><topic>Immunoglobulin G - chemistry</topic><topic>Immunoglobulin G - ultrastructure</topic><topic>Interfaces: Adsorption, Reactions, Films, Forces</topic><topic>Microscopy, Atomic Force</topic><topic>Serum Albumin - chemistry</topic><topic>Surface physical chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holmberg, Maria</creatorcontrib><creatorcontrib>Hou, Xiaolin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holmberg, Maria</au><au>Hou, Xiaolin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competitive Protein AdsorptionMultilayer Adsorption and Surface Induced Protein Aggregation</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2009-02-17</date><risdate>2009</risdate><volume>25</volume><issue>4</issue><spage>2081</spage><epage>2089</epage><pages>2081-2089</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>In this study, competitive adsorption of albumin and IgG (immunoglobulin G) from human serum solutions and protein mixtures onto polymer surfaces is studied by means of radioactive labeling. By using two different radiolabels (125I and 131I), albumin and IgG adsorption to polymer surfaces is monitored simultaneously and the influence from the presence of other human serum proteins on albumin and IgG adsorption, as well as their mutual influence during adsorption processes, is investigated. Exploring protein adsorption by combining analysis of competitive adsorption from complex solutions of high concentration with investigation of single protein adsorption and interdependent adsorption between two specific proteins enables us to map protein adsorption sequences during competitive protein adsorption. Our study shows that proteins can adsorb in a multilayer fashion onto the polymer surfaces and that the outcome of IgG adsorption is much more sensitive to surface characteristics than the outcome of albumin adsorption. 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| ispartof | Langmuir, 2009-02, Vol.25 (4), p.2081-2089 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Adsorption Chemistry Colloidal state and disperse state Exact sciences and technology General and physical chemistry Humans Immunoglobulin G - chemistry Immunoglobulin G - ultrastructure Interfaces: Adsorption, Reactions, Films, Forces Microscopy, Atomic Force Serum Albumin - chemistry Surface physical chemistry |
| title | Competitive Protein AdsorptionMultilayer Adsorption and Surface Induced Protein Aggregation |
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