Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells
In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). Accordi...
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| Veröffentlicht in: | Food and chemical toxicology 2004-12, Vol.42 (12), p.1937-1947 |
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| creator | Wu, C.-C. Chung, J.G. Tsai, S.-J. Yang, J.H. Sheen, L.Y. |
| description | In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 μM DATS significantly decreased the cell viability as compared with the control (PDAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides. |
| doi_str_mv | 10.1016/j.fct.2004.07.008 |
| format | Article |
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According to the results of cell viability assay, 50 or 100 μM DATS significantly decreased the cell viability as compared with the control (P<0.05) in dose and time dependent relations. Phenomena of cell number loss, shape deformation and lysis were observed after treatment with 100 μM DATS for 24 h. Cell cycle studies showed that J5 cells were significantly arrested in G2/M phase as the cells were treated with 100 μM DADS, 10, 50 or 100 μM DATS for 24 h (P<0.05). DATS was more effective in arresting cells in G2/M phase than DADS, and the phenomena of arresting J5 cells in G2/M phase increased obviously in dose and time dependent relations. According to the Western blot analysis, DATS decreased cyclin-dependent kinase (Cdks)-Cdk7 (i.e. Cdc2 activate kinase) protein levels in J5 cells but increased cyclin B1 protein level. The modulation potency to cyclin B1 and Cdk7 expressions was in the order of DATS>DADS>DAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2004.07.008</identifier><identifier>PMID: 15500931</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Allium ; Allium sativum ; Allyl Compounds - pharmacology ; allyl sulfur compounds ; anti-proliferation ; anticarcinogenic activity ; Antineoplastic Agents, Phytogenic ; apoptosis ; Biological and medical sciences ; Blotting, Western ; cell culture ; Cell cycle ; Cell Cycle - drug effects ; cell cycle arrest ; Cell Line, Tumor ; cell lines ; Cell Survival - drug effects ; chemical constituents of plants ; chemical structure ; chemoprevention ; Cyclin-Dependent Kinases - biosynthesis ; cyclins ; Cyclins - biosynthesis ; Diallyl disulfide ; diallyl disulfide (DADS) ; Diallyl sulfide ; diallyl sulfides ; Diallyl trisulfide ; diallyl trisulfide (DATS) ; Disulfides - pharmacology ; Electrophoresis, Polyacrylamide Gel ; essential oils ; Garlic ; Garlic - chemistry ; Human liver tumor cells ; Humans ; liver neoplasms ; Liver Neoplasms - drug therapy ; Medical sciences ; Neoplasm Proteins - biosynthesis ; nutrition-genotype interaction ; Oils, Volatile - pharmacology ; plant extracts ; Sulfides - pharmacology ; Toxicology ; viability</subject><ispartof>Food and chemical toxicology, 2004-12, Vol.42 (12), p.1937-1947</ispartof><rights>2004 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4588-df63b79717756ae83f4c0e891ea4e53aa454d8dac0563ac86a39ee933922c55d3</citedby><cites>FETCH-LOGICAL-c4588-df63b79717756ae83f4c0e891ea4e53aa454d8dac0563ac86a39ee933922c55d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2004.07.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16259243$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15500931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, C.-C.</creatorcontrib><creatorcontrib>Chung, J.G.</creatorcontrib><creatorcontrib>Tsai, S.-J.</creatorcontrib><creatorcontrib>Yang, J.H.</creatorcontrib><creatorcontrib>Sheen, L.Y.</creatorcontrib><title>Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 μM DATS significantly decreased the cell viability as compared with the control (P<0.05) in dose and time dependent relations. Phenomena of cell number loss, shape deformation and lysis were observed after treatment with 100 μM DATS for 24 h. Cell cycle studies showed that J5 cells were significantly arrested in G2/M phase as the cells were treated with 100 μM DADS, 10, 50 or 100 μM DATS for 24 h (P<0.05). DATS was more effective in arresting cells in G2/M phase than DADS, and the phenomena of arresting J5 cells in G2/M phase increased obviously in dose and time dependent relations. According to the Western blot analysis, DATS decreased cyclin-dependent kinase (Cdks)-Cdk7 (i.e. Cdc2 activate kinase) protein levels in J5 cells but increased cyclin B1 protein level. The modulation potency to cyclin B1 and Cdk7 expressions was in the order of DATS>DADS>DAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides.</description><subject>Allium</subject><subject>Allium sativum</subject><subject>Allyl Compounds - pharmacology</subject><subject>allyl sulfur compounds</subject><subject>anti-proliferation</subject><subject>anticarcinogenic activity</subject><subject>Antineoplastic Agents, Phytogenic</subject><subject>apoptosis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>cell culture</subject><subject>Cell cycle</subject><subject>Cell Cycle - drug effects</subject><subject>cell cycle arrest</subject><subject>Cell Line, Tumor</subject><subject>cell lines</subject><subject>Cell Survival - drug effects</subject><subject>chemical constituents of plants</subject><subject>chemical structure</subject><subject>chemoprevention</subject><subject>Cyclin-Dependent Kinases - biosynthesis</subject><subject>cyclins</subject><subject>Cyclins - biosynthesis</subject><subject>Diallyl disulfide</subject><subject>diallyl disulfide (DADS)</subject><subject>Diallyl sulfide</subject><subject>diallyl sulfides</subject><subject>Diallyl trisulfide</subject><subject>diallyl trisulfide (DATS)</subject><subject>Disulfides - pharmacology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>essential oils</subject><subject>Garlic</subject><subject>Garlic - chemistry</subject><subject>Human liver tumor cells</subject><subject>Humans</subject><subject>liver neoplasms</subject><subject>Liver Neoplasms - drug therapy</subject><subject>Medical sciences</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>nutrition-genotype interaction</subject><subject>Oils, Volatile - pharmacology</subject><subject>plant extracts</subject><subject>Sulfides - pharmacology</subject><subject>Toxicology</subject><subject>viability</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhB3ABX-CWMI5jxxYnVD6lShygZ2vqjBevnKTYSaX993jZSL3BwbJlPfPq1TxV9ZJDw4Grd4fGu6VpAboG-gZAP6p2XPeiVkLyx9UO2l7XynB5UT3L-QAAPe_V0-qCSwlgBN9Vy8fgPSWaloCRUXm7JbPZM4zxGFleow8DZebTPLI9phgco5w3fg7lTMxRjMwdXSSWaL9GXEL5DRP7tY44sRjuKbFlHef0F83PqyceY6YX231Z3Xz-9PPqa339_cu3qw_Xteuk1vXglbjtTencS4Wkhe8ckDacsCMpEDvZDXpAB1IJdFqhMERGCNO2TspBXFZvz7l3af69Ul7sGPKpAU40r9mqHkCVJf0XLA2UBGMKyM-gS3POiby9S2HEdLQc7MmJPdjixJ6cWOhtcVJmXm3h6-1Iw8PEJqEAbzYAs8PoE04u5AdOtdK0nSjc6zPncba4T4W5-dECFyVGGa1P9d6fCSpbvQ-UbHaBJkdDSEWsHebwj6J_AHi5s5k</recordid><startdate>200412</startdate><enddate>200412</enddate><creator>Wu, C.-C.</creator><creator>Chung, J.G.</creator><creator>Tsai, S.-J.</creator><creator>Yang, J.H.</creator><creator>Sheen, L.Y.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200412</creationdate><title>Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells</title><author>Wu, C.-C. ; Chung, J.G. ; Tsai, S.-J. ; Yang, J.H. ; Sheen, L.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4588-df63b79717756ae83f4c0e891ea4e53aa454d8dac0563ac86a39ee933922c55d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Allium</topic><topic>Allium sativum</topic><topic>Allyl Compounds - pharmacology</topic><topic>allyl sulfur compounds</topic><topic>anti-proliferation</topic><topic>anticarcinogenic activity</topic><topic>Antineoplastic Agents, Phytogenic</topic><topic>apoptosis</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>cell culture</topic><topic>Cell cycle</topic><topic>Cell Cycle - drug effects</topic><topic>cell cycle arrest</topic><topic>Cell Line, Tumor</topic><topic>cell lines</topic><topic>Cell Survival - drug effects</topic><topic>chemical constituents of plants</topic><topic>chemical structure</topic><topic>chemoprevention</topic><topic>Cyclin-Dependent Kinases - biosynthesis</topic><topic>cyclins</topic><topic>Cyclins - biosynthesis</topic><topic>Diallyl disulfide</topic><topic>diallyl disulfide (DADS)</topic><topic>Diallyl sulfide</topic><topic>diallyl sulfides</topic><topic>Diallyl trisulfide</topic><topic>diallyl trisulfide (DATS)</topic><topic>Disulfides - pharmacology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>essential oils</topic><topic>Garlic</topic><topic>Garlic - chemistry</topic><topic>Human liver tumor cells</topic><topic>Humans</topic><topic>liver neoplasms</topic><topic>Liver Neoplasms - drug therapy</topic><topic>Medical sciences</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>nutrition-genotype interaction</topic><topic>Oils, Volatile - pharmacology</topic><topic>plant extracts</topic><topic>Sulfides - pharmacology</topic><topic>Toxicology</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, C.-C.</creatorcontrib><creatorcontrib>Chung, J.G.</creatorcontrib><creatorcontrib>Tsai, S.-J.</creatorcontrib><creatorcontrib>Yang, J.H.</creatorcontrib><creatorcontrib>Sheen, L.Y.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, C.-C.</au><au>Chung, J.G.</au><au>Tsai, S.-J.</au><au>Yang, J.H.</au><au>Sheen, L.Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2004-12</date><risdate>2004</risdate><volume>42</volume><issue>12</issue><spage>1937</spage><epage>1947</epage><pages>1937-1947</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 μM DATS significantly decreased the cell viability as compared with the control (P<0.05) in dose and time dependent relations. Phenomena of cell number loss, shape deformation and lysis were observed after treatment with 100 μM DATS for 24 h. Cell cycle studies showed that J5 cells were significantly arrested in G2/M phase as the cells were treated with 100 μM DADS, 10, 50 or 100 μM DATS for 24 h (P<0.05). DATS was more effective in arresting cells in G2/M phase than DADS, and the phenomena of arresting J5 cells in G2/M phase increased obviously in dose and time dependent relations. According to the Western blot analysis, DATS decreased cyclin-dependent kinase (Cdks)-Cdk7 (i.e. Cdc2 activate kinase) protein levels in J5 cells but increased cyclin B1 protein level. The modulation potency to cyclin B1 and Cdk7 expressions was in the order of DATS>DADS>DAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>15500931</pmid><doi>10.1016/j.fct.2004.07.008</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Allium Allium sativum Allyl Compounds - pharmacology allyl sulfur compounds anti-proliferation anticarcinogenic activity Antineoplastic Agents, Phytogenic apoptosis Biological and medical sciences Blotting, Western cell culture Cell cycle Cell Cycle - drug effects cell cycle arrest Cell Line, Tumor cell lines Cell Survival - drug effects chemical constituents of plants chemical structure chemoprevention Cyclin-Dependent Kinases - biosynthesis cyclins Cyclins - biosynthesis Diallyl disulfide diallyl disulfide (DADS) Diallyl sulfide diallyl sulfides Diallyl trisulfide diallyl trisulfide (DATS) Disulfides - pharmacology Electrophoresis, Polyacrylamide Gel essential oils Garlic Garlic - chemistry Human liver tumor cells Humans liver neoplasms Liver Neoplasms - drug therapy Medical sciences Neoplasm Proteins - biosynthesis nutrition-genotype interaction Oils, Volatile - pharmacology plant extracts Sulfides - pharmacology Toxicology viability |
| title | Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells |
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