Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells

In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). Accordi...

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Veröffentlicht in:	Food and chemical toxicology 2004-12, Vol.42 (12), p.1937-1947
Hauptverfasser:	Wu, C.-C., Chung, J.G., Tsai, S.-J., Yang, J.H., Sheen, L.Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allium Allium sativum Allyl Compounds - pharmacology allyl sulfur compounds anti-proliferation anticarcinogenic activity Antineoplastic Agents, Phytogenic apoptosis Biological and medical sciences Blotting, Western cell culture Cell cycle Cell Cycle - drug effects cell cycle arrest Cell Line, Tumor cell lines Cell Survival - drug effects chemical constituents of plants chemical structure chemoprevention Cyclin-Dependent Kinases - biosynthesis cyclins Cyclins - biosynthesis Diallyl disulfide diallyl disulfide (DADS) Diallyl sulfide diallyl sulfides Diallyl trisulfide diallyl trisulfide (DATS) Disulfides - pharmacology Electrophoresis, Polyacrylamide Gel essential oils Garlic Garlic - chemistry Human liver tumor cells Humans liver neoplasms Liver Neoplasms - drug therapy Medical sciences Neoplasm Proteins - biosynthesis nutrition-genotype interaction Oils, Volatile - pharmacology plant extracts Sulfides - pharmacology Toxicology viability
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Zusammenfassung:	In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 μM DATS significantly decreased the cell viability as compared with the control (PDAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides.
ISSN:	0278-6915 1873-6351
DOI:	10.1016/j.fct.2004.07.008