Hyperexpression of Low-Density Lipoprotein Receptors and Hydroxy-Methylglutaryl-Coenzyme A-Reductase in Human Pinguecula and Primary Pterygium
There is now increasing evidence that pterygium and pinguecula are tumorlike tissues and that cell growth and DNA replication are closely linked to cholesterol metabolism. In this study, the expression of two main genes correlated to cholesterol metabolism--namely, the low-density lipoprotein recept...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2004-11, Vol.45 (11), p.3982-3985 |
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| creator | Peiretti, Enrico Dessi, Sandra Putzolu, Marirosa Fossarello, Maurizio |
| description | There is now increasing evidence that pterygium and pinguecula are tumorlike tissues and that cell growth and DNA replication are closely linked to cholesterol metabolism. In this study, the expression of two main genes correlated to cholesterol metabolism--namely, the low-density lipoprotein receptor (LDL-R) gene and the hydroxy-methylglutaryl-coenzyme A-reductase (HMG-CoA-R) gene--were investigated in primary pterygium, pinguecula, and normal conjunctiva.
Pterygium, pinguecula, and normal conjunctiva samples were obtained from 30 eyes (50% men) at the time of surgery. Total RNA extracted from the specimens was subjected to semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Equal amounts of total RNA were reverse transcribed into cDNA. The cDNA was subsequently amplified by the PCR in the presence of specific primers for low-density lipoprotein receptor (LDL-R) and for hydroxy-methylglutaryl coenzyme A reductase (HMG-CoA-R).
Semiquantitative RT-PCR analysis revealed that the mRNA levels of LDL-R and HMG-CoA-R were increased in pterygia, compared with levels in both pingueculae and normal conjunctivae. Differences were statistically significant (P |
| doi_str_mv | 10.1167/iovs.04-0176 |
| format | Article |
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Pterygium, pinguecula, and normal conjunctiva samples were obtained from 30 eyes (50% men) at the time of surgery. Total RNA extracted from the specimens was subjected to semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Equal amounts of total RNA were reverse transcribed into cDNA. The cDNA was subsequently amplified by the PCR in the presence of specific primers for low-density lipoprotein receptor (LDL-R) and for hydroxy-methylglutaryl coenzyme A reductase (HMG-CoA-R).
Semiquantitative RT-PCR analysis revealed that the mRNA levels of LDL-R and HMG-CoA-R were increased in pterygia, compared with levels in both pingueculae and normal conjunctivae. Differences were statistically significant (P <0.05), including pingueculae versus normal conjunctivae.
This study indicates that pterygium and pinguecula have an altered metabolism of cholesterol-namely increased LDL-R and HMG-CoA-R mRNAs-as is characteristic of tumorlike tissues, and that the high expression of LDL receptors renders them amenable to be treated by photodynamic therapy with intravenously injected verteporfin.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.04-0176</identifier><identifier>PMID: 15505045</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Biological and medical sciences ; Cholesterol - metabolism ; Conjunctival Diseases - metabolism ; Connective Tissue Diseases - metabolism ; Diseases of eyelid, conjunctiva and lacrimal tracts ; Eye and associated structures. Visual pathways and centers. Vision ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; Hydroxymethylglutaryl CoA Reductases - genetics ; Male ; Medical sciences ; Middle Aged ; Ophthalmology ; Pterygium - metabolism ; Receptors, LDL - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - isolation & purification ; RNA, Messenger - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 2004-11, Vol.45 (11), p.3982-3985</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-ab61a7dfb6605df2a1eb4aa88334231671d24029cac80290c23b96f0396dc1d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16219393$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15505045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peiretti, Enrico</creatorcontrib><creatorcontrib>Dessi, Sandra</creatorcontrib><creatorcontrib>Putzolu, Marirosa</creatorcontrib><creatorcontrib>Fossarello, Maurizio</creatorcontrib><title>Hyperexpression of Low-Density Lipoprotein Receptors and Hydroxy-Methylglutaryl-Coenzyme A-Reductase in Human Pinguecula and Primary Pterygium</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>There is now increasing evidence that pterygium and pinguecula are tumorlike tissues and that cell growth and DNA replication are closely linked to cholesterol metabolism. In this study, the expression of two main genes correlated to cholesterol metabolism--namely, the low-density lipoprotein receptor (LDL-R) gene and the hydroxy-methylglutaryl-coenzyme A-reductase (HMG-CoA-R) gene--were investigated in primary pterygium, pinguecula, and normal conjunctiva.
Pterygium, pinguecula, and normal conjunctiva samples were obtained from 30 eyes (50% men) at the time of surgery. Total RNA extracted from the specimens was subjected to semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Equal amounts of total RNA were reverse transcribed into cDNA. The cDNA was subsequently amplified by the PCR in the presence of specific primers for low-density lipoprotein receptor (LDL-R) and for hydroxy-methylglutaryl coenzyme A reductase (HMG-CoA-R).
Semiquantitative RT-PCR analysis revealed that the mRNA levels of LDL-R and HMG-CoA-R were increased in pterygia, compared with levels in both pingueculae and normal conjunctivae. Differences were statistically significant (P <0.05), including pingueculae versus normal conjunctivae.
This study indicates that pterygium and pinguecula have an altered metabolism of cholesterol-namely increased LDL-R and HMG-CoA-R mRNAs-as is characteristic of tumorlike tissues, and that the high expression of LDL receptors renders them amenable to be treated by photodynamic therapy with intravenously injected verteporfin.</description><subject>Biological and medical sciences</subject><subject>Cholesterol - metabolism</subject><subject>Conjunctival Diseases - metabolism</subject><subject>Connective Tissue Diseases - metabolism</subject><subject>Diseases of eyelid, conjunctiva and lacrimal tracts</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Hydroxymethylglutaryl CoA Reductases - genetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Ophthalmology</subject><subject>Pterygium - metabolism</subject><subject>Receptors, LDL - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - isolation & purification</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE2P0zAQhi0EYsvCjTPKBbjgxd9NjqvyUaQiqtXeLceZtEZOHOyEbPgR_GZcWmlPc3nmfWcehF5TckOpWn904Xe6IQITulZP0IpKybBcl_wpWhEqFCaCiCv0IqWfhDBKGXmOrjJEJBFyhf5ulwEiPAwRUnKhL0Jb7MKMP0Gf3LgUOzeEIYYRXF_cgYVhDDEVpm-K7dLE8LDg7zAeF3_w02ji4vEmQP9n6aC4xXfQTHY0CYq8vJ060xd71x8msJM3_zP20XV5q9iPEJeDm7qX6FlrfIJXl3mN7r98vt9s8e7H12-b2x22gsoRm1pRs27aWikim5YZCrUwpiw5F4xnK7RhgrDKGlvmQSzjdaVawivVWNrwa_TuHJtf-zVBGnXnkgXvTQ9hSlqtCZGl5Bn8cAZtDClFaPVwPllTok_69Um_JkKf9Gf8zSV3qjtoHuGL7wy8vQAmWePbaHrr0iOnGK14dep9f-aO7nCcXQSdOuN9jqV6nmchc7nmVcn4P9K7npU</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Peiretti, Enrico</creator><creator>Dessi, Sandra</creator><creator>Putzolu, Marirosa</creator><creator>Fossarello, Maurizio</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Hyperexpression of Low-Density Lipoprotein Receptors and Hydroxy-Methylglutaryl-Coenzyme A-Reductase in Human Pinguecula and Primary Pterygium</title><author>Peiretti, Enrico ; Dessi, Sandra ; Putzolu, Marirosa ; Fossarello, Maurizio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-ab61a7dfb6605df2a1eb4aa88334231671d24029cac80290c23b96f0396dc1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Cholesterol - metabolism</topic><topic>Conjunctival Diseases - metabolism</topic><topic>Connective Tissue Diseases - metabolism</topic><topic>Diseases of eyelid, conjunctiva and lacrimal tracts</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Hydroxymethylglutaryl CoA Reductases - genetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Ophthalmology</topic><topic>Pterygium - metabolism</topic><topic>Receptors, LDL - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - isolation & purification</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peiretti, Enrico</creatorcontrib><creatorcontrib>Dessi, Sandra</creatorcontrib><creatorcontrib>Putzolu, Marirosa</creatorcontrib><creatorcontrib>Fossarello, Maurizio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peiretti, Enrico</au><au>Dessi, Sandra</au><au>Putzolu, Marirosa</au><au>Fossarello, Maurizio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyperexpression of Low-Density Lipoprotein Receptors and Hydroxy-Methylglutaryl-Coenzyme A-Reductase in Human Pinguecula and Primary Pterygium</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>45</volume><issue>11</issue><spage>3982</spage><epage>3985</epage><pages>3982-3985</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>There is now increasing evidence that pterygium and pinguecula are tumorlike tissues and that cell growth and DNA replication are closely linked to cholesterol metabolism. In this study, the expression of two main genes correlated to cholesterol metabolism--namely, the low-density lipoprotein receptor (LDL-R) gene and the hydroxy-methylglutaryl-coenzyme A-reductase (HMG-CoA-R) gene--were investigated in primary pterygium, pinguecula, and normal conjunctiva.
Pterygium, pinguecula, and normal conjunctiva samples were obtained from 30 eyes (50% men) at the time of surgery. Total RNA extracted from the specimens was subjected to semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Equal amounts of total RNA were reverse transcribed into cDNA. The cDNA was subsequently amplified by the PCR in the presence of specific primers for low-density lipoprotein receptor (LDL-R) and for hydroxy-methylglutaryl coenzyme A reductase (HMG-CoA-R).
Semiquantitative RT-PCR analysis revealed that the mRNA levels of LDL-R and HMG-CoA-R were increased in pterygia, compared with levels in both pingueculae and normal conjunctivae. Differences were statistically significant (P <0.05), including pingueculae versus normal conjunctivae.
This study indicates that pterygium and pinguecula have an altered metabolism of cholesterol-namely increased LDL-R and HMG-CoA-R mRNAs-as is characteristic of tumorlike tissues, and that the high expression of LDL receptors renders them amenable to be treated by photodynamic therapy with intravenously injected verteporfin.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>15505045</pmid><doi>10.1167/iovs.04-0176</doi><tpages>4</tpages></addata></record> |
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| subjects | Biological and medical sciences Cholesterol - metabolism Conjunctival Diseases - metabolism Connective Tissue Diseases - metabolism Diseases of eyelid, conjunctiva and lacrimal tracts Eye and associated structures. Visual pathways and centers. Vision Female Fundamental and applied biological sciences. Psychology Gene Expression Humans Hydroxymethylglutaryl CoA Reductases - genetics Male Medical sciences Middle Aged Ophthalmology Pterygium - metabolism Receptors, LDL - genetics Reverse Transcriptase Polymerase Chain Reaction RNA - isolation & purification RNA, Messenger - metabolism Vertebrates: nervous system and sense organs |
| title | Hyperexpression of Low-Density Lipoprotein Receptors and Hydroxy-Methylglutaryl-Coenzyme A-Reductase in Human Pinguecula and Primary Pterygium |
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