Suppression of apoptosis in perfusion culture of Myeloma NS0 cells enhances cell growth but reduces antibody productivity

A spin filter perfusion systems was used to achieve a high cell density culture for two NS0 cell lines in 2 litres bioreactors. One cell line is transfected with the bcl-2 gene (NS0 Bcl-2) encodes the 'anti-apoptotic' human Bcl-2 protein and the other cell line (NS0 Control) with a blank v...

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Bibliographische Detailangaben
Veröffentlicht in:	Apoptosis (London) 2004-11, Vol.9 (6), p.843-852
Hauptverfasser:	Tey, B T, Al-Rubeai, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - biosynthesis Apoptosis Bacteria Bioreactors Cell Count Cell Culture Techniques Cell cycle Cell Proliferation Cell Survival Culture Media - analysis Enzyme-Linked Immunosorbent Assay Glucose - analysis Humans Hybridomas - cytology Immunoglobulin G - analysis Microscopy, Confocal Mortality Multiple Myeloma - immunology Multiple Myeloma - pathology Proteins Proto-Oncogene Proteins c-bcl-2 - analysis Proto-Oncogene Proteins c-bcl-2 - genetics Time Factors Transfection
Online-Zugang:	Volltext
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