Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia
Burkholderia cepacia complex strains are opportunistic pathogens causing life-threatening infections in cystic fibrosis patients. B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes Bu...
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| Veröffentlicht in: | Research in microbiology 2009-03, Vol.160 (2), p.144-151 |
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| creator | Brackman, Gilles Hillaert, Ulrik Van Calenbergh, Serge Nelis, Hans J. Coenye, Tom |
| description | Burkholderia cepacia complex strains are opportunistic pathogens causing life-threatening infections in cystic fibrosis patients.
B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes
Burkholderia infections difficult to treat. Recently, the quorum sensing (QS) system of
Burkholderia spp. has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. However, detailed information about the anti-biofilm effect of these compounds is still lacking. In the present study, we evaluated the anti-biofilm effect of several known QS inhibitors. The effect on
Burkholderia spp. biofilm formation was examined using crystal violet, resazurin and SYTO9 staining, confocal laser scanning microscopy as well as plating. When used at subinhibitory concentrations, several compounds interfered with biofilm formation by
Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment. |
| doi_str_mv | 10.1016/j.resmic.2008.12.003 |
| format | Article |
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B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes
Burkholderia infections difficult to treat. Recently, the quorum sensing (QS) system of
Burkholderia spp. has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. However, detailed information about the anti-biofilm effect of these compounds is still lacking. In the present study, we evaluated the anti-biofilm effect of several known QS inhibitors. The effect on
Burkholderia spp. biofilm formation was examined using crystal violet, resazurin and SYTO9 staining, confocal laser scanning microscopy as well as plating. When used at subinhibitory concentrations, several compounds interfered with biofilm formation by
Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/j.resmic.2008.12.003</identifier><identifier>PMID: 19146953</identifier><language>eng</language><publisher>Issy-les-Moulineaux: Elsevier SAS</publisher><subject>Anti-Infective Agents - pharmacology ; Bacterial Adhesion - drug effects ; Bacteriology ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; Burkholderia cepacia ; Burkholderia cepacia - physiology ; Burkholderia cepacia complex ; Burkholderia multivorans ; Colony Count, Microbial ; Confocal laser scanning microscopy ; Cystic Fibrosis - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbial Sensitivity Tests ; Microbiology ; Microscopy, Confocal ; Miscellaneous ; Quorum sensing ; Quorum Sensing - drug effects</subject><ispartof>Research in microbiology, 2009-03, Vol.160 (2), p.144-151</ispartof><rights>2009 Elsevier Masson SAS</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-a4733cd82f4943702847fdcb487968d2b0df81d47f65bf84be011d2f0e3c2edb3</citedby><cites>FETCH-LOGICAL-c467t-a4733cd82f4943702847fdcb487968d2b0df81d47f65bf84be011d2f0e3c2edb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0923250808002234$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21299066$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19146953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brackman, Gilles</creatorcontrib><creatorcontrib>Hillaert, Ulrik</creatorcontrib><creatorcontrib>Van Calenbergh, Serge</creatorcontrib><creatorcontrib>Nelis, Hans J.</creatorcontrib><creatorcontrib>Coenye, Tom</creatorcontrib><title>Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>Burkholderia cepacia complex strains are opportunistic pathogens causing life-threatening infections in cystic fibrosis patients.
B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes
Burkholderia infections difficult to treat. Recently, the quorum sensing (QS) system of
Burkholderia spp. has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. However, detailed information about the anti-biofilm effect of these compounds is still lacking. In the present study, we evaluated the anti-biofilm effect of several known QS inhibitors. The effect on
Burkholderia spp. biofilm formation was examined using crystal violet, resazurin and SYTO9 staining, confocal laser scanning microscopy as well as plating. When used at subinhibitory concentrations, several compounds interfered with biofilm formation by
Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment.</description><subject>Anti-Infective Agents - pharmacology</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Burkholderia cepacia</subject><subject>Burkholderia cepacia - physiology</subject><subject>Burkholderia cepacia complex</subject><subject>Burkholderia multivorans</subject><subject>Colony Count, Microbial</subject><subject>Confocal laser scanning microscopy</subject><subject>Cystic Fibrosis - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Microscopy, Confocal</subject><subject>Miscellaneous</subject><subject>Quorum sensing</subject><subject>Quorum Sensing - drug effects</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEURonROO3oGxjDRndVAkUDtTHRiX_JJG6cNaHgYtNWQQ9QbXwI31nG7mjc6IoA57u5-Q5CTynpKaHi5b7PUJZge0aI6inrCRnuoQ2VYuwkZcN9tCEjGzq2JeoCPSplTwjdSskfogs6Ui7G7bBBP24K4OTx7ZryuuACsYT4BYe4C1OoKRdcU7tVyB4y4G-h7vAUkg_zgn3Ki6khRWyiww6OMKfDArG2AH6z5q-7NDvIweBlnWs4pmxi-cX-9WkhJgsHY4N5jB54Mxd4cj4v0c27t5-vPnTXn95_vHp93VkuZO0Ml8NgnWKej3yQhCkuvbMTV3IUyrGJOK-oa49iO3nFJyCUOuYJDJaBm4ZL9OI095DT7Qql6iUUC_NsIqS1aCFbV6qV-D-QEU6kUqSB_ATanErJ4PUhh8Xk75oSfedL7_XJl77zpSnTzVeLPTvPX6cF3J_QWVADnp8BU6yZfavQhvKbY5SNIxGica9OHLTajgGyLjZAtOBCBlu1S-Hfm_wENku5Lw</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Brackman, Gilles</creator><creator>Hillaert, Ulrik</creator><creator>Van Calenbergh, Serge</creator><creator>Nelis, Hans J.</creator><creator>Coenye, Tom</creator><general>Elsevier SAS</general><general>Elsevier Masson</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia</title><author>Brackman, Gilles ; Hillaert, Ulrik ; Van Calenbergh, Serge ; Nelis, Hans J. ; Coenye, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-a4733cd82f4943702847fdcb487968d2b0df81d47f65bf84be011d2f0e3c2edb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anti-Infective Agents - pharmacology</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Burkholderia cepacia</topic><topic>Burkholderia cepacia - physiology</topic><topic>Burkholderia cepacia complex</topic><topic>Burkholderia multivorans</topic><topic>Colony Count, Microbial</topic><topic>Confocal laser scanning microscopy</topic><topic>Cystic Fibrosis - microbiology</topic><topic>Fundamental and applied biological sciences. 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B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes
Burkholderia infections difficult to treat. Recently, the quorum sensing (QS) system of
Burkholderia spp. has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. However, detailed information about the anti-biofilm effect of these compounds is still lacking. In the present study, we evaluated the anti-biofilm effect of several known QS inhibitors. The effect on
Burkholderia spp. biofilm formation was examined using crystal violet, resazurin and SYTO9 staining, confocal laser scanning microscopy as well as plating. When used at subinhibitory concentrations, several compounds interfered with biofilm formation by
Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment.</abstract><cop>Issy-les-Moulineaux</cop><pub>Elsevier SAS</pub><pmid>19146953</pmid><doi>10.1016/j.resmic.2008.12.003</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Infective Agents - pharmacology Bacterial Adhesion - drug effects Bacteriology Biofilms Biofilms - drug effects Biofilms - growth & development Biological and medical sciences Burkholderia cepacia Burkholderia cepacia - physiology Burkholderia cepacia complex Burkholderia multivorans Colony Count, Microbial Confocal laser scanning microscopy Cystic Fibrosis - microbiology Fundamental and applied biological sciences. Psychology Humans Microbial Sensitivity Tests Microbiology Microscopy, Confocal Miscellaneous Quorum sensing Quorum Sensing - drug effects |
| title | Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia |
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