Microscale mechanical properties of single elastic fibers: The role of fibrillin–microfibrils
Abstract Micromechanical properties of single elastic fibers and fibrillin–microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or conta...
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| Veröffentlicht in: | Biomaterials 2009-05, Vol.30 (13), p.2425-2432 |
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| creator | Koenders, Mieke M.J.F Yang, Lanti Wismans, Ronnie G van der Werf, Kees O Reinhardt, Dieter P Daamen, Willeke Bennink, Martin L Dijkstra, Pieter J van Kuppevelt, Toin H Feijen, Jan |
| description | Abstract Micromechanical properties of single elastic fibers and fibrillin–microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin–microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3–1.5 MPa were determined, values not significantly affected by the presence of fibrillin–microfibrils. To further understand the role of fibrillin–microfibrils in vertebrate elastic fibers, layers of fibrillin–microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin–microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers. |
| doi_str_mv | 10.1016/j.biomaterials.2009.01.038 |
| format | Article |
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Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin–microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3–1.5 MPa were determined, values not significantly affected by the presence of fibrillin–microfibrils. To further understand the role of fibrillin–microfibrils in vertebrate elastic fibers, layers of fibrillin–microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin–microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2009.01.038</identifier><identifier>PMID: 19217657</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; AFM (atomic force microscopy) ; Dentistry ; Elastic Tissue - chemistry ; Elastic Tissue - ultrastructure ; Elastin ; Fibrillins ; Mechanical properties ; Mechanical test ; Microfibrils - chemistry ; Microfibrils - metabolism ; Microfibrils - ultrastructure ; Microfilament Proteins - chemistry ; Microfilament Proteins - metabolism ; Microfilament Proteins - ultrastructure ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Nano-indentation ; Stress, Mechanical</subject><ispartof>Biomaterials, 2009-05, Vol.30 (13), p.2425-2432</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-1d3f1fb94bb576c27377f5fc0ef924a5889966fc2e317eb62f5acecc0dbd9e2a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014296120900088X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19217657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koenders, Mieke M.J.F</creatorcontrib><creatorcontrib>Yang, Lanti</creatorcontrib><creatorcontrib>Wismans, Ronnie G</creatorcontrib><creatorcontrib>van der Werf, Kees O</creatorcontrib><creatorcontrib>Reinhardt, Dieter P</creatorcontrib><creatorcontrib>Daamen, Willeke</creatorcontrib><creatorcontrib>Bennink, Martin L</creatorcontrib><creatorcontrib>Dijkstra, Pieter J</creatorcontrib><creatorcontrib>van Kuppevelt, Toin H</creatorcontrib><creatorcontrib>Feijen, Jan</creatorcontrib><title>Microscale mechanical properties of single elastic fibers: The role of fibrillin–microfibrils</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Micromechanical properties of single elastic fibers and fibrillin–microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin–microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3–1.5 MPa were determined, values not significantly affected by the presence of fibrillin–microfibrils. To further understand the role of fibrillin–microfibrils in vertebrate elastic fibers, layers of fibrillin–microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin–microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.</description><subject>Advanced Basic Science</subject><subject>AFM (atomic force microscopy)</subject><subject>Dentistry</subject><subject>Elastic Tissue - chemistry</subject><subject>Elastic Tissue - ultrastructure</subject><subject>Elastin</subject><subject>Fibrillins</subject><subject>Mechanical properties</subject><subject>Mechanical test</subject><subject>Microfibrils - chemistry</subject><subject>Microfibrils - metabolism</subject><subject>Microfibrils - ultrastructure</subject><subject>Microfilament Proteins - chemistry</subject><subject>Microfilament Proteins - metabolism</subject><subject>Microfilament Proteins - ultrastructure</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nano-indentation</subject><subject>Stress, Mechanical</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAQxy0EotvCK6CIA7eEGSe24x6QUIGCVMSBInGzHGdMveRjsbNIvfEOvCFPgqNdCcSlnDye-c2H5j-MPUWoEFA-31ZdmEe7UAx2SBUH0BVgBXV7j22wVW0pNIj7bAPY8FJL5CfsNKUt5D80_CE7Qc1RSaE2zLwPLs7J2YGKkdyNnUK2i12cdxSXQKmYfZHC9CXHabBpCa7woaOYzovrGyrinAMZyb4YhiFMv378HNeSB0d6xB74PCQ9Pr5n7NOb19cXb8urD5fvLl5ela7RYimxrz36TjddJ5R0XNVKeeEdkNe8saJttZbSO041Kuok98I6cg76rtfEbX3Gnh3q5sm_7SktZgzJ0TDYieZ9MlJqrbSCO8E6d0YO6k6QQ4MoxAqeH8B1kSmSN7sYRhtvDYJZBTNb87dgZhXMAJosWE5-cuyy70bq_6QeFcrAqwNAeXvfA0WTXKDJUR8iucX0c_i_Pi_-KeOyWKvWX-mW0nbex2nNQZO4AfNxPZ31ckADQNt-rn8DmB7F2Q</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Koenders, Mieke M.J.F</creator><creator>Yang, Lanti</creator><creator>Wismans, Ronnie G</creator><creator>van der Werf, Kees O</creator><creator>Reinhardt, Dieter P</creator><creator>Daamen, Willeke</creator><creator>Bennink, Martin L</creator><creator>Dijkstra, Pieter J</creator><creator>van Kuppevelt, Toin H</creator><creator>Feijen, Jan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Microscale mechanical properties of single elastic fibers: The role of fibrillin–microfibrils</title><author>Koenders, Mieke M.J.F ; Yang, Lanti ; Wismans, Ronnie G ; van der Werf, Kees O ; Reinhardt, Dieter P ; Daamen, Willeke ; Bennink, Martin L ; Dijkstra, Pieter J ; van Kuppevelt, Toin H ; Feijen, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-1d3f1fb94bb576c27377f5fc0ef924a5889966fc2e317eb62f5acecc0dbd9e2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Advanced Basic Science</topic><topic>AFM (atomic force microscopy)</topic><topic>Dentistry</topic><topic>Elastic Tissue - chemistry</topic><topic>Elastic Tissue - ultrastructure</topic><topic>Elastin</topic><topic>Fibrillins</topic><topic>Mechanical properties</topic><topic>Mechanical test</topic><topic>Microfibrils - chemistry</topic><topic>Microfibrils - metabolism</topic><topic>Microfibrils - ultrastructure</topic><topic>Microfilament Proteins - chemistry</topic><topic>Microfilament Proteins - metabolism</topic><topic>Microfilament Proteins - ultrastructure</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nano-indentation</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koenders, Mieke M.J.F</creatorcontrib><creatorcontrib>Yang, Lanti</creatorcontrib><creatorcontrib>Wismans, Ronnie G</creatorcontrib><creatorcontrib>van der Werf, Kees O</creatorcontrib><creatorcontrib>Reinhardt, Dieter P</creatorcontrib><creatorcontrib>Daamen, Willeke</creatorcontrib><creatorcontrib>Bennink, Martin L</creatorcontrib><creatorcontrib>Dijkstra, Pieter J</creatorcontrib><creatorcontrib>van Kuppevelt, Toin H</creatorcontrib><creatorcontrib>Feijen, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koenders, Mieke M.J.F</au><au>Yang, Lanti</au><au>Wismans, Ronnie G</au><au>van der Werf, Kees O</au><au>Reinhardt, Dieter P</au><au>Daamen, Willeke</au><au>Bennink, Martin L</au><au>Dijkstra, Pieter J</au><au>van Kuppevelt, Toin H</au><au>Feijen, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microscale mechanical properties of single elastic fibers: The role of fibrillin–microfibrils</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>30</volume><issue>13</issue><spage>2425</spage><epage>2432</epage><pages>2425-2432</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Micromechanical properties of single elastic fibers and fibrillin–microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin–microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3–1.5 MPa were determined, values not significantly affected by the presence of fibrillin–microfibrils. To further understand the role of fibrillin–microfibrils in vertebrate elastic fibers, layers of fibrillin–microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin–microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19217657</pmid><doi>10.1016/j.biomaterials.2009.01.038</doi><tpages>8</tpages></addata></record> |
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| subjects | Advanced Basic Science AFM (atomic force microscopy) Dentistry Elastic Tissue - chemistry Elastic Tissue - ultrastructure Elastin Fibrillins Mechanical properties Mechanical test Microfibrils - chemistry Microfibrils - metabolism Microfibrils - ultrastructure Microfilament Proteins - chemistry Microfilament Proteins - metabolism Microfilament Proteins - ultrastructure Microscopy, Atomic Force Microscopy, Electron, Scanning Nano-indentation Stress, Mechanical |
| title | Microscale mechanical properties of single elastic fibers: The role of fibrillin–microfibrils |
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