Rapid Induction of Splenic and Peritoneal B-1a Cells in Adult Mice by Thymus-Independent Type-2 Antigen
We have produced a transgenic mouse (PV1TgL) that can only generate B lymphocytes with an Ig receptor specific for the synthetic polymer polyvinyl pyrrolidinone. Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the...
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| Veröffentlicht in: | The Journal of immunology (1950) 2004-11, Vol.173 (9), p.5406-5414 |
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| container_title | The Journal of immunology (1950) |
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| creator | Whitmore, Alan C Neely, Harold R Diz, Ramiro Flood, Patrick M |
| description | We have produced a transgenic mouse (PV1TgL) that can only generate B lymphocytes with an Ig receptor specific for the synthetic polymer polyvinyl pyrrolidinone. Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the role of positive selection by Ag in the development of mature B cell populations. The predominant population of B cells in the spleens of naive adult PV1TgL mice have most of the characteristics of marginal zone B cells, including anatomical location in the peripheral areas of the splenic white pulp. After immunization, a new population of B cells appears in the spleen with the characteristics of B-1 cells. Similar cells also appear somewhat later in the peritoneal cavity. Our findings suggest that immunization with a thymus-independent Ag can lead to the appearance and expansion of Ag-reactive B-1 cells in an adult mouse. |
| doi_str_mv | 10.4049/jimmunol.173.9.5406 |
| format | Article |
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Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the role of positive selection by Ag in the development of mature B cell populations. The predominant population of B cells in the spleens of naive adult PV1TgL mice have most of the characteristics of marginal zone B cells, including anatomical location in the peripheral areas of the splenic white pulp. After immunization, a new population of B cells appears in the spleen with the characteristics of B-1 cells. Similar cells also appear somewhat later in the peritoneal cavity. 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Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the role of positive selection by Ag in the development of mature B cell populations. The predominant population of B cells in the spleens of naive adult PV1TgL mice have most of the characteristics of marginal zone B cells, including anatomical location in the peripheral areas of the splenic white pulp. After immunization, a new population of B cells appears in the spleen with the characteristics of B-1 cells. Similar cells also appear somewhat later in the peritoneal cavity. Our findings suggest that immunization with a thymus-independent Ag can lead to the appearance and expansion of Ag-reactive B-1 cells in an adult mouse.</description><subject>Aging - genetics</subject><subject>Aging - immunology</subject><subject>Animals</subject><subject>Antibody Formation - genetics</subject><subject>Antigens, T-Independent - administration & dosage</subject><subject>Antigens, T-Independent - immunology</subject><subject>B-Lymphocyte Subsets - cytology</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Differentiation - immunology</subject><subject>Crosses, Genetic</subject><subject>Female</subject><subject>Immunization - methods</subject><subject>Immunophenotyping</subject><subject>Lymphocyte Activation - genetics</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Mice, Transgenic</subject><subject>Peritoneum - cytology</subject><subject>Peritoneum - immunology</subject><subject>Povidone - administration & dosage</subject><subject>Povidone - analogs & derivatives</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1ERZfCEyAhn-gpy9hxnPVxWbW0UhEIlrPl2JNdV44T4kSrffu62q3gxmXm8v2fRvMT8oHBUoBQnx99182xD0tWl0u1rATIV2TBqgoKKUG-JgsAzgtWy_qSvE3pEQAkcPGGXLJKKCFW9YLsfprBO3of3Wwn30fat_TXEDB6S0109AeOfuojmkC_FMzQDYaQqI907eYw0W_eIm2OdLs_dnMqsgYHzCNOdHscsOB0HSe_w_iOXLQmJHx_3lfk9-3NdnNXPHz_er9ZPxRWgJqKqmykNbyUbYNmpRANciWNAuWM5VA7KYVbKUCwJXDJlLNclpVreKNc27TlFfl08g5j_2fGNOnOJ5uPNhH7OWkpleJSsf-CbJWfV0qRwfIE2rFPacRWD6PvzHjUDPRzEfqlCJ2L0Eo_F5FTH8_6uenQ_c2cP5-B6xOw97v9wY-oU2dCyDjTh8PhH9UTyI6TzQ</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Whitmore, Alan C</creator><creator>Neely, Harold R</creator><creator>Diz, Ramiro</creator><creator>Flood, Patrick M</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Rapid Induction of Splenic and Peritoneal B-1a Cells in Adult Mice by Thymus-Independent Type-2 Antigen</title><author>Whitmore, Alan C ; Neely, Harold R ; Diz, Ramiro ; Flood, Patrick M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-53b6ca236fbea89eeae296a909dac207d664d890e0c302619dc2635db2b9dfbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aging - genetics</topic><topic>Aging - immunology</topic><topic>Animals</topic><topic>Antibody Formation - genetics</topic><topic>Antigens, T-Independent - administration & dosage</topic><topic>Antigens, T-Independent - immunology</topic><topic>B-Lymphocyte Subsets - cytology</topic><topic>B-Lymphocyte Subsets - immunology</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Differentiation - immunology</topic><topic>Crosses, Genetic</topic><topic>Female</topic><topic>Immunization - methods</topic><topic>Immunophenotyping</topic><topic>Lymphocyte Activation - genetics</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Mice, Transgenic</topic><topic>Peritoneum - cytology</topic><topic>Peritoneum - immunology</topic><topic>Povidone - administration & dosage</topic><topic>Povidone - analogs & derivatives</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whitmore, Alan C</creatorcontrib><creatorcontrib>Neely, Harold R</creatorcontrib><creatorcontrib>Diz, Ramiro</creatorcontrib><creatorcontrib>Flood, Patrick M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whitmore, Alan C</au><au>Neely, Harold R</au><au>Diz, Ramiro</au><au>Flood, Patrick M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Induction of Splenic and Peritoneal B-1a Cells in Adult Mice by Thymus-Independent Type-2 Antigen</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>173</volume><issue>9</issue><spage>5406</spage><epage>5414</epage><pages>5406-5414</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>We have produced a transgenic mouse (PV1TgL) that can only generate B lymphocytes with an Ig receptor specific for the synthetic polymer polyvinyl pyrrolidinone. Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the role of positive selection by Ag in the development of mature B cell populations. The predominant population of B cells in the spleens of naive adult PV1TgL mice have most of the characteristics of marginal zone B cells, including anatomical location in the peripheral areas of the splenic white pulp. After immunization, a new population of B cells appears in the spleen with the characteristics of B-1 cells. Similar cells also appear somewhat later in the peritoneal cavity. Our findings suggest that immunization with a thymus-independent Ag can lead to the appearance and expansion of Ag-reactive B-1 cells in an adult mouse.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>15494487</pmid><doi>10.4049/jimmunol.173.9.5406</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aging - genetics Aging - immunology Animals Antibody Formation - genetics Antigens, T-Independent - administration & dosage Antigens, T-Independent - immunology B-Lymphocyte Subsets - cytology B-Lymphocyte Subsets - immunology Cell Differentiation - genetics Cell Differentiation - immunology Crosses, Genetic Female Immunization - methods Immunophenotyping Lymphocyte Activation - genetics Lymphocyte Activation - immunology Lymphocyte Count Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Peritoneum - cytology Peritoneum - immunology Povidone - administration & dosage Povidone - analogs & derivatives Spleen - cytology Spleen - immunology |
| title | Rapid Induction of Splenic and Peritoneal B-1a Cells in Adult Mice by Thymus-Independent Type-2 Antigen |
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