WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis

BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor prot...

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Veröffentlicht in:Human reproduction (Oxford) 2004-11, Vol.19 (11), p.2594-2604
Hauptverfasser: Rawe, Vanesa Y., Ramalho-Santos, João, Payne, Christopher, Chemes, Hector E., Schatten, Gerald
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container_end_page 2604
container_issue 11
container_start_page 2594
container_title Human reproduction (Oxford)
container_volume 19
creator Rawe, Vanesa Y.
Ramalho-Santos, João
Payne, Christopher
Chemes, Hector E.
Schatten, Gerald
description BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm. CONCLUSIONS: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm.
doi_str_mv 10.1093/humrep/deh513
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In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. 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Teratology</topic><topic>Epididymis - cytology</topic><topic>Epididymis - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Golgi</topic><topic>Golgi Apparatus - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Mammals - physiology</topic><topic>Mice</topic><topic>Microfilament Proteins - metabolism</topic><topic>mitochondrial sheath</topic><topic>Papio</topic><topic>Proteins - metabolism</topic><topic>rac1 GTP-Binding Protein - metabolism</topic><topic>sperm</topic><topic>Spermatogenesis - physiology</topic><topic>Spermatozoa - metabolism</topic><topic>WAVE1</topic><topic>Wiskott-Aldrich Syndrome Protein Family</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawe, Vanesa Y.</creatorcontrib><creatorcontrib>Ramalho-Santos, João</creatorcontrib><creatorcontrib>Payne, Christopher</creatorcontrib><creatorcontrib>Chemes, Hector E.</creatorcontrib><creatorcontrib>Schatten, Gerald</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawe, Vanesa Y.</au><au>Ramalho-Santos, João</au><au>Payne, Christopher</au><au>Chemes, Hector E.</au><au>Schatten, Gerald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. 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source MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals
subjects AKAP
Animals
Biological and medical sciences
Cattle
Cell Compartmentation
Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit
Cyclic AMP-Dependent Protein Kinases - metabolism
Embryology: invertebrates and vertebrates. Teratology
Epididymis - cytology
Epididymis - physiology
Fundamental and applied biological sciences. Psychology
Golgi
Golgi Apparatus - metabolism
Humans
Male
Mammals - physiology
Mice
Microfilament Proteins - metabolism
mitochondrial sheath
Papio
Proteins - metabolism
rac1 GTP-Binding Protein - metabolism
sperm
Spermatogenesis - physiology
Spermatozoa - metabolism
WAVE1
Wiskott-Aldrich Syndrome Protein Family
title WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis
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