WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis
BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor prot...
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Veröffentlicht in: | Human reproduction (Oxford) 2004-11, Vol.19 (11), p.2594-2604 |
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description | BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm. CONCLUSIONS: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm. |
doi_str_mv | 10.1093/humrep/deh513 |
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In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm. CONCLUSIONS: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/deh513</identifier><identifier>PMID: 15471936</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>AKAP ; Animals ; Biological and medical sciences ; Cattle ; Cell Compartmentation ; Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Embryology: invertebrates and vertebrates. Teratology ; Epididymis - cytology ; Epididymis - physiology ; Fundamental and applied biological sciences. Psychology ; Golgi ; Golgi Apparatus - metabolism ; Humans ; Male ; Mammals - physiology ; Mice ; Microfilament Proteins - metabolism ; mitochondrial sheath ; Papio ; Proteins - metabolism ; rac1 GTP-Binding Protein - metabolism ; sperm ; Spermatogenesis - physiology ; Spermatozoa - metabolism ; WAVE1 ; Wiskott-Aldrich Syndrome Protein Family</subject><ispartof>Human reproduction (Oxford), 2004-11, Vol.19 (11), p.2594-2604</ispartof><rights>Human Reproduction vol. 19 no. 11 © European Society of Human Reproduction and Embryology 2004; all rights reserved 2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Nov 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-81a7c506351b3e81948200086e1bd86d20a40c89d73aca310319df49a6d2df513</citedby><cites>FETCH-LOGICAL-c522t-81a7c506351b3e81948200086e1bd86d20a40c89d73aca310319df49a6d2df513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16225536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15471936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rawe, Vanesa Y.</creatorcontrib><creatorcontrib>Ramalho-Santos, João</creatorcontrib><creatorcontrib>Payne, Christopher</creatorcontrib><creatorcontrib>Chemes, Hector E.</creatorcontrib><creatorcontrib>Schatten, Gerald</creatorcontrib><title>WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm. CONCLUSIONS: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm.</description><subject>AKAP</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Compartmentation</subject><subject>Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Epididymis - cytology</subject><subject>Epididymis - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Golgi</subject><subject>Golgi Apparatus - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Mammals - physiology</subject><subject>Mice</subject><subject>Microfilament Proteins - metabolism</subject><subject>mitochondrial sheath</subject><subject>Papio</subject><subject>Proteins - metabolism</subject><subject>rac1 GTP-Binding Protein - metabolism</subject><subject>sperm</subject><subject>Spermatogenesis - physiology</subject><subject>Spermatozoa - metabolism</subject><subject>WAVE1</subject><subject>Wiskott-Aldrich Syndrome Protein Family</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MtL7DAUB-Agio6P5d3KIFxxYTUnadJkOQyOo4yI4Iu7CZk2daJ93aQF_e-Ntii4cZUT8nFyzg-hP4BPAEt6uupKZ5rTzKwY0DU0gpjjiFCG19EIEy4iAA5baNv7Z4xDKfgm2gIWJyApH6HZw-T-DI7HuhpPohdbaW9Cna5qZ6uncePq1tjqeJx1n_dSl6UubMC-Ma7Ubf1kKuOt30UbuS682RvOHXQ3O7udzqPF9fnFdLKIUkZIGwnQScowpwyW1AiQsSAYY8ENLDPBM4J1jFMhs4TqVFPAFGSWx1KHpywPC-6gw75vmOx_Z3yrSutTUxS6MnXnFedSAsUf8OAHfK47V4XZFAEQgsg4DijqUepq753JVeNsqd2bAqw-0lV9uqpPN_j9oWm3LE32rYc4A_g7AO1TXeQuRGn9t-OEMPbpjnpXd82vfw4zWt-a1y-s3YviCU2Ymj_-U1dwM1_Mbom6pO-zSZ8Z</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Rawe, Vanesa Y.</creator><creator>Ramalho-Santos, João</creator><creator>Payne, Christopher</creator><creator>Chemes, Hector E.</creator><creator>Schatten, Gerald</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis</title><author>Rawe, Vanesa Y. ; Ramalho-Santos, João ; Payne, Christopher ; Chemes, Hector E. ; Schatten, Gerald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-81a7c506351b3e81948200086e1bd86d20a40c89d73aca310319df49a6d2df513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>AKAP</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Compartmentation</topic><topic>Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Epididymis - cytology</topic><topic>Epididymis - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Golgi</topic><topic>Golgi Apparatus - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Mammals - physiology</topic><topic>Mice</topic><topic>Microfilament Proteins - metabolism</topic><topic>mitochondrial sheath</topic><topic>Papio</topic><topic>Proteins - metabolism</topic><topic>rac1 GTP-Binding Protein - metabolism</topic><topic>sperm</topic><topic>Spermatogenesis - physiology</topic><topic>Spermatozoa - metabolism</topic><topic>WAVE1</topic><topic>Wiskott-Aldrich Syndrome Protein Family</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawe, Vanesa Y.</creatorcontrib><creatorcontrib>Ramalho-Santos, João</creatorcontrib><creatorcontrib>Payne, Christopher</creatorcontrib><creatorcontrib>Chemes, Hector E.</creatorcontrib><creatorcontrib>Schatten, Gerald</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawe, Vanesa Y.</au><au>Ramalho-Santos, João</au><au>Payne, Christopher</au><au>Chemes, Hector E.</au><au>Schatten, Gerald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>19</volume><issue>11</issue><spage>2594</spage><epage>2604</epage><pages>2594-2604</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: Proper compartmentalization of signalling cascades is paramount to many intracellular activities during spermatogenesis and sperm function. In the present study we focus on the A-kinase-anchoring protein (AKAP) WAVE1, a member of the Wiskott–Aldrich syndrome (WASP) family of adaptor proteins, to study its localization throughout mammalian spermatogenesis. METHODS: Using transmission electron microscopy, immunocytochemistry and western blotting, we examined the distribution of WAVE1 and putative partners during mammalian spermatogenesis. The localization and association of PKA RII, the regulatory subunit II of protein kinase A, tyrosine kinase Abl, and small GTPase RAC1 were also explored. RESULTS: WAVE1 localization in spermatocytes and round spermatids coincided with Golgi apparatus distribution, whereas in elongated spermatids and testicular sperm WAVE1 localized to the mitochondrial sheath. Following epididymal passage, WAVE1 was found exclusively on the mitochondrial sheath, suggesting that the protein may function in this region. WAVE1 and PKA RII co-localized along the mitochondrial sheath, PKA RII concentrates in the mid-piece, and RAC1 associated with the post-acrosomal region and the connecting piece. The distribution of WAVE1, PKA RII and RAC1 is conserved in mature mouse, bull, baboon and human sperm. CONCLUSIONS: The data support the possibility of a functional signalling unit established by WAVE1 and its associated proteins in the mid-piece of maturing sperm.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15471936</pmid><doi>10.1093/humrep/deh513</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
subjects | AKAP Animals Biological and medical sciences Cattle Cell Compartmentation Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit Cyclic AMP-Dependent Protein Kinases - metabolism Embryology: invertebrates and vertebrates. Teratology Epididymis - cytology Epididymis - physiology Fundamental and applied biological sciences. Psychology Golgi Golgi Apparatus - metabolism Humans Male Mammals - physiology Mice Microfilament Proteins - metabolism mitochondrial sheath Papio Proteins - metabolism rac1 GTP-Binding Protein - metabolism sperm Spermatogenesis - physiology Spermatozoa - metabolism WAVE1 Wiskott-Aldrich Syndrome Protein Family |
title | WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis |
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