Characterization of ionizing radiation-induced ring chromosomes by atomic force microscopy
We applied atomic force microscopy (AFM) to the structural analysis of radiation-induced ring chromosomes. Constrictions observed on the metaphase ring chromosome were found to correspond to the centromere regions of the ring chromosome in comparison with the AFM image of the centromere of rod chrom...
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| Veröffentlicht in: | Analytical biochemistry 2004-11, Vol.334 (2), p.251-256 |
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| container_title | Analytical biochemistry |
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| creator | Murakami, Masahiro Kanda, Reiko Minamihisamatsu, Masako Hayata, Isamu |
| description | We applied atomic force microscopy (AFM) to the structural analysis of radiation-induced ring chromosomes. Constrictions observed on the metaphase ring chromosome were found to correspond to the centromere regions of the ring chromosome in comparison with the AFM image of the centromere of rod chromosomes and with the fluorescence in situ hybridization (FISH) technique. Section analysis by AFM revealed that some ring-like chromosome fragments and ring-like chromatid fragments were thicker than standard chromosomes or chromatids, suggesting that they were ring chromosomes viewed edge on. Topographic analysis by AFM makes it possible to distinguish a ring viewed edge on that is difficult to recognize as a ring by light microscopy and to discriminate between a centric ring chromosome and an acentric ring chromosome using the same slides prepared for light microscopy. |
| doi_str_mv | 10.1016/j.ab.2004.07.023 |
| format | Article |
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Constrictions observed on the metaphase ring chromosome were found to correspond to the centromere regions of the ring chromosome in comparison with the AFM image of the centromere of rod chromosomes and with the fluorescence in situ hybridization (FISH) technique. Section analysis by AFM revealed that some ring-like chromosome fragments and ring-like chromatid fragments were thicker than standard chromosomes or chromatids, suggesting that they were ring chromosomes viewed edge on. Topographic analysis by AFM makes it possible to distinguish a ring viewed edge on that is difficult to recognize as a ring by light microscopy and to discriminate between a centric ring chromosome and an acentric ring chromosome using the same slides prepared for light microscopy.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2004.07.023</identifier><identifier>PMID: 15494131</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Atomic force microscopy ; Centromere ; Centromere - radiation effects ; Centromere - ultrastructure ; Chromosome aberration ; Chromosomes, Human - radiation effects ; Chromosomes, Human - ultrastructure ; Female ; Humans ; Metaphase - radiation effects ; Microscopy, Atomic Force ; Prematurely condensed chromosome ; Radiation, Ionizing ; Ring chromosome ; Ring Chromosomes</subject><ispartof>Analytical biochemistry, 2004-11, Vol.334 (2), p.251-256</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-6220dee68e3ede3f497f3aa631741c86389fd544539832342fa352b631aa53bc3</citedby><cites>FETCH-LOGICAL-c421t-6220dee68e3ede3f497f3aa631741c86389fd544539832342fa352b631aa53bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2004.07.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15494131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murakami, Masahiro</creatorcontrib><creatorcontrib>Kanda, Reiko</creatorcontrib><creatorcontrib>Minamihisamatsu, Masako</creatorcontrib><creatorcontrib>Hayata, Isamu</creatorcontrib><title>Characterization of ionizing radiation-induced ring chromosomes by atomic force microscopy</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>We applied atomic force microscopy (AFM) to the structural analysis of radiation-induced ring chromosomes. Constrictions observed on the metaphase ring chromosome were found to correspond to the centromere regions of the ring chromosome in comparison with the AFM image of the centromere of rod chromosomes and with the fluorescence in situ hybridization (FISH) technique. Section analysis by AFM revealed that some ring-like chromosome fragments and ring-like chromatid fragments were thicker than standard chromosomes or chromatids, suggesting that they were ring chromosomes viewed edge on. Topographic analysis by AFM makes it possible to distinguish a ring viewed edge on that is difficult to recognize as a ring by light microscopy and to discriminate between a centric ring chromosome and an acentric ring chromosome using the same slides prepared for light microscopy.</description><subject>Atomic force microscopy</subject><subject>Centromere</subject><subject>Centromere - radiation effects</subject><subject>Centromere - ultrastructure</subject><subject>Chromosome aberration</subject><subject>Chromosomes, Human - radiation effects</subject><subject>Chromosomes, Human - ultrastructure</subject><subject>Female</subject><subject>Humans</subject><subject>Metaphase - radiation effects</subject><subject>Microscopy, Atomic Force</subject><subject>Prematurely condensed chromosome</subject><subject>Radiation, Ionizing</subject><subject>Ring chromosome</subject><subject>Ring Chromosomes</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtPwzAQhy0EgvLYmVAmtoTzI07MhipeEhILLCyW41zAVRMXO0Vq_3pcWokJMd3p7rufdB8h5xQKClRezQrTFAxAFFAVwPgemVBQMgcOap9MAIDnTKrqiBzHOAOgVJTykBzRUihBOZ2Qt-mHCcaOGNzajM4Pme-yVNzaDe9ZMK37meZuaJcW2yxsxvYj-N5H32PMmlVmRt87m3U-WMxSF3y0frE6JQedmUc829UT8np3-zJ9yJ-e7x-nN0-5FYyOuWQMWkRZI8cWeSdU1XFjJKeVoLaWvFZdWwpRclVzxgXrDC9Zk_bGlLyx_IRcbnMXwX8uMY66d9HifG4G9MuopVQKKg7_grRidSXqMoGwBTevxICdXgTXm7DSFPRGvJ5p0-iNeA2VTuLTycUue9n02P4e7Ewn4HoLYFLx5TDoaB0OyakLaEfdevd3-jeTqJMG</recordid><startdate>20041115</startdate><enddate>20041115</enddate><creator>Murakami, Masahiro</creator><creator>Kanda, Reiko</creator><creator>Minamihisamatsu, Masako</creator><creator>Hayata, Isamu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041115</creationdate><title>Characterization of ionizing radiation-induced ring chromosomes by atomic force microscopy</title><author>Murakami, Masahiro ; Kanda, Reiko ; Minamihisamatsu, Masako ; Hayata, Isamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-6220dee68e3ede3f497f3aa631741c86389fd544539832342fa352b631aa53bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Atomic force microscopy</topic><topic>Centromere</topic><topic>Centromere - radiation effects</topic><topic>Centromere - ultrastructure</topic><topic>Chromosome aberration</topic><topic>Chromosomes, Human - radiation effects</topic><topic>Chromosomes, Human - ultrastructure</topic><topic>Female</topic><topic>Humans</topic><topic>Metaphase - radiation effects</topic><topic>Microscopy, Atomic Force</topic><topic>Prematurely condensed chromosome</topic><topic>Radiation, Ionizing</topic><topic>Ring chromosome</topic><topic>Ring Chromosomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murakami, Masahiro</creatorcontrib><creatorcontrib>Kanda, Reiko</creatorcontrib><creatorcontrib>Minamihisamatsu, Masako</creatorcontrib><creatorcontrib>Hayata, Isamu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murakami, Masahiro</au><au>Kanda, Reiko</au><au>Minamihisamatsu, Masako</au><au>Hayata, Isamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of ionizing radiation-induced ring chromosomes by atomic force microscopy</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2004-11-15</date><risdate>2004</risdate><volume>334</volume><issue>2</issue><spage>251</spage><epage>256</epage><pages>251-256</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>We applied atomic force microscopy (AFM) to the structural analysis of radiation-induced ring chromosomes. Constrictions observed on the metaphase ring chromosome were found to correspond to the centromere regions of the ring chromosome in comparison with the AFM image of the centromere of rod chromosomes and with the fluorescence in situ hybridization (FISH) technique. Section analysis by AFM revealed that some ring-like chromosome fragments and ring-like chromatid fragments were thicker than standard chromosomes or chromatids, suggesting that they were ring chromosomes viewed edge on. Topographic analysis by AFM makes it possible to distinguish a ring viewed edge on that is difficult to recognize as a ring by light microscopy and to discriminate between a centric ring chromosome and an acentric ring chromosome using the same slides prepared for light microscopy.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15494131</pmid><doi>10.1016/j.ab.2004.07.023</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Freedom Collection (Elsevier) |
| subjects | Atomic force microscopy Centromere Centromere - radiation effects Centromere - ultrastructure Chromosome aberration Chromosomes, Human - radiation effects Chromosomes, Human - ultrastructure Female Humans Metaphase - radiation effects Microscopy, Atomic Force Prematurely condensed chromosome Radiation, Ionizing Ring chromosome Ring Chromosomes |
| title | Characterization of ionizing radiation-induced ring chromosomes by atomic force microscopy |
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