Liquid chromatographic determination of St. John's wort components in functional foods
A method was developed for determination of St. John's wort marker compounds hypericin, pseudohypericin, hyperforin, and adhyperforin in functional foods. Solid-phase extraction provided analyte extraction and significant sample cleanup prior to analysis using liquid chromatography (LC) with UV...
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| Veröffentlicht in: | Journal of AOAC International 2004-09, Vol.87 (5), p.1042-1048 |
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| container_title | Journal of AOAC International |
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| creator | De Jager, L.S Perfetti, G.A Diachenko, G.W |
| description | A method was developed for determination of St. John's wort marker compounds hypericin, pseudohypericin, hyperforin, and adhyperforin in functional foods. Solid-phase extraction provided analyte extraction and significant sample cleanup prior to analysis using liquid chromatography (LC) with UV and fluorescence detection. In addition to quantification using LC-UV, confirmation was made with electrospray ionization LC mass spectrometry (LC/MS). Several commercially available tea and drink products claiming to contain St. John's wort were tested. Recoveries ranged from 51 to 98% for the liquid samples. Comparison of the concentrations in 4 St. John's wort teas showed a variation in analyte concentration (1044-10 ng/mL marker compounds in brewed tea) and composition. No marker compounds were found in the beverages, indicating possible decomposition of the marker compounds caused by low pH and/or exposure to light. A solvent extraction procedure was developed for analysis of the marker compounds from solid samples. Analytes were detected at low parts per million, with an average recovery of 75%. No St. John's wort components were found in the 2 solid functional food samples analyzed. |
| doi_str_mv | 10.1093/jaoac/87.5.1042 |
| format | Article |
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Solid-phase extraction provided analyte extraction and significant sample cleanup prior to analysis using liquid chromatography (LC) with UV and fluorescence detection. In addition to quantification using LC-UV, confirmation was made with electrospray ionization LC mass spectrometry (LC/MS). Several commercially available tea and drink products claiming to contain St. John's wort were tested. Recoveries ranged from 51 to 98% for the liquid samples. Comparison of the concentrations in 4 St. John's wort teas showed a variation in analyte concentration (1044-10 ng/mL marker compounds in brewed tea) and composition. No marker compounds were found in the beverages, indicating possible decomposition of the marker compounds caused by low pH and/or exposure to light. A solvent extraction procedure was developed for analysis of the marker compounds from solid samples. Analytes were detected at low parts per million, with an average recovery of 75%. No St. John's wort components were found in the 2 solid functional food samples analyzed.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.1093/jaoac/87.5.1042</identifier><identifier>PMID: 15493659</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>adhyperforin ; beverages ; Beverages - analysis ; Biological and medical sciences ; Bridged Bicyclo Compounds - analysis ; Chromatography, Liquid ; Food Analysis ; functional foods ; General pharmacology ; herbal tea ; hyperforin ; hypericin ; Hypericum perforatum ; liquid chromatography ; Medical sciences ; Perylene - analogs & derivatives ; Perylene - analysis ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phloroglucinol - analogs & derivatives ; Phloroglucinol - analysis ; pseudohypericin ; quinones ; solid phase extraction ; Terpenes - analysis</subject><ispartof>Journal of AOAC International, 2004-09, Vol.87 (5), p.1042-1048</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-2235ffd28ef1abbe5afe56ba8c746748978f1e6b4db06a657d52d65f00f50cdf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16120190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15493659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Jager, L.S</creatorcontrib><creatorcontrib>Perfetti, G.A</creatorcontrib><creatorcontrib>Diachenko, G.W</creatorcontrib><title>Liquid chromatographic determination of St. John's wort components in functional foods</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A method was developed for determination of St. John's wort marker compounds hypericin, pseudohypericin, hyperforin, and adhyperforin in functional foods. Solid-phase extraction provided analyte extraction and significant sample cleanup prior to analysis using liquid chromatography (LC) with UV and fluorescence detection. In addition to quantification using LC-UV, confirmation was made with electrospray ionization LC mass spectrometry (LC/MS). Several commercially available tea and drink products claiming to contain St. John's wort were tested. Recoveries ranged from 51 to 98% for the liquid samples. Comparison of the concentrations in 4 St. John's wort teas showed a variation in analyte concentration (1044-10 ng/mL marker compounds in brewed tea) and composition. No marker compounds were found in the beverages, indicating possible decomposition of the marker compounds caused by low pH and/or exposure to light. A solvent extraction procedure was developed for analysis of the marker compounds from solid samples. Analytes were detected at low parts per million, with an average recovery of 75%. No St. John's wort components were found in the 2 solid functional food samples analyzed.</description><subject>adhyperforin</subject><subject>beverages</subject><subject>Beverages - analysis</subject><subject>Biological and medical sciences</subject><subject>Bridged Bicyclo Compounds - analysis</subject><subject>Chromatography, Liquid</subject><subject>Food Analysis</subject><subject>functional foods</subject><subject>General pharmacology</subject><subject>herbal tea</subject><subject>hyperforin</subject><subject>hypericin</subject><subject>Hypericum perforatum</subject><subject>liquid chromatography</subject><subject>Medical sciences</subject><subject>Perylene - analogs & derivatives</subject><subject>Perylene - analysis</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phloroglucinol - analogs & derivatives</subject><subject>Phloroglucinol - analysis</subject><subject>pseudohypericin</subject><subject>quinones</subject><subject>solid phase extraction</subject><subject>Terpenes - analysis</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0MtvGyEQB2BUtWoSt-feWi5NT2vzhj1GUV-RpRyS9IpYHjHRLjiwqyr_fXFtKSdAfDOj-QHwCaM1Rj3dPJls7EbJNW9vRt6Ac9wz1smekLftjgTqKJH4DFzU-oQQwwKR9-AMc9ZTwftz8Gcbn5fooN2VPJk5Pxaz30ULnZ99mWIyc8wJ5gDv5jW8ybv0rcK_uczQ5mmfk09zhTHBsCR7kGaEIWdXP4B3wYzVfzydK_Dw4_v99a9ue_vz9_XVtrNUqbkjhPIQHFE-YDMMnpvguRiMspIJyVQvVcBeDMwNSBjBpePECR4QChxZF-gKXB777kt-Xnyd9RSr9eNoks9L1UL0iveINrg5QltyrcUHvS9xMuVFY6QPUer_UWolNdeHKFvF51PrZZi8e_Wn7Br4egKmWjOGYpKN9dUJTBBus1fgy9EFk7V5LM083LUvilDbTyJG_wFxfYev</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>De Jager, L.S</creator><creator>Perfetti, G.A</creator><creator>Diachenko, G.W</creator><general>AOAC International</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Liquid chromatographic determination of St. John's wort components in functional foods</title><author>De Jager, L.S ; Perfetti, G.A ; Diachenko, G.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-2235ffd28ef1abbe5afe56ba8c746748978f1e6b4db06a657d52d65f00f50cdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>adhyperforin</topic><topic>beverages</topic><topic>Beverages - analysis</topic><topic>Biological and medical sciences</topic><topic>Bridged Bicyclo Compounds - analysis</topic><topic>Chromatography, Liquid</topic><topic>Food Analysis</topic><topic>functional foods</topic><topic>General pharmacology</topic><topic>herbal tea</topic><topic>hyperforin</topic><topic>hypericin</topic><topic>Hypericum perforatum</topic><topic>liquid chromatography</topic><topic>Medical sciences</topic><topic>Perylene - analogs & derivatives</topic><topic>Perylene - analysis</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phloroglucinol - analogs & derivatives</topic><topic>Phloroglucinol - analysis</topic><topic>pseudohypericin</topic><topic>quinones</topic><topic>solid phase extraction</topic><topic>Terpenes - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Jager, L.S</creatorcontrib><creatorcontrib>Perfetti, G.A</creatorcontrib><creatorcontrib>Diachenko, G.W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of AOAC International</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Jager, L.S</au><au>Perfetti, G.A</au><au>Diachenko, G.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid chromatographic determination of St. John's wort components in functional foods</atitle><jtitle>Journal of AOAC International</jtitle><addtitle>J AOAC Int</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>87</volume><issue>5</issue><spage>1042</spage><epage>1048</epage><pages>1042-1048</pages><issn>1060-3271</issn><eissn>1944-7922</eissn><abstract>A method was developed for determination of St. John's wort marker compounds hypericin, pseudohypericin, hyperforin, and adhyperforin in functional foods. Solid-phase extraction provided analyte extraction and significant sample cleanup prior to analysis using liquid chromatography (LC) with UV and fluorescence detection. In addition to quantification using LC-UV, confirmation was made with electrospray ionization LC mass spectrometry (LC/MS). Several commercially available tea and drink products claiming to contain St. John's wort were tested. Recoveries ranged from 51 to 98% for the liquid samples. Comparison of the concentrations in 4 St. John's wort teas showed a variation in analyte concentration (1044-10 ng/mL marker compounds in brewed tea) and composition. No marker compounds were found in the beverages, indicating possible decomposition of the marker compounds caused by low pH and/or exposure to light. A solvent extraction procedure was developed for analysis of the marker compounds from solid samples. Analytes were detected at low parts per million, with an average recovery of 75%. No St. John's wort components were found in the 2 solid functional food samples analyzed.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>15493659</pmid><doi>10.1093/jaoac/87.5.1042</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Oxford University Press Journals; MEDLINE |
| subjects | adhyperforin beverages Beverages - analysis Biological and medical sciences Bridged Bicyclo Compounds - analysis Chromatography, Liquid Food Analysis functional foods General pharmacology herbal tea hyperforin hypericin Hypericum perforatum liquid chromatography Medical sciences Perylene - analogs & derivatives Perylene - analysis Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phloroglucinol - analogs & derivatives Phloroglucinol - analysis pseudohypericin quinones solid phase extraction Terpenes - analysis |
| title | Liquid chromatographic determination of St. John's wort components in functional foods |
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