Distribution and subcellular localization of a water-soluble hematoporphyrin–platinum(II) complex in human bladder cancer cells
The water-soluble porphyrin–platinum complex diammine{7,12-bis[1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato}platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates an...
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| Veröffentlicht in: | Cancer letters 2004-11, Vol.215 (2), p.167-177 |
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| container_title | Cancer letters |
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| creator | Lottner, Christian Knuechel, Ruth Bernhardt, Guenther Brunner, Henri |
| description | The water-soluble porphyrin–platinum complex diammine{7,12-bis[1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato}platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates and degree of DNA platination on the low-differentiated J82 cells, a model of invasive bladder cancer, and UROtsa, a normal urothelial cell line. Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 cancer cells than in UROtsa cells. Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA platination in J82 and UROtsa cells was higher by PEG-HPPt than by cisplatin, whereas there was no significant difference between the two cell lines. |
| doi_str_mv | 10.1016/j.canlet.2004.06.035 |
| format | Article |
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Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 cancer cells than in UROtsa cells. Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA platination in J82 and UROtsa cells was higher by PEG-HPPt than by cisplatin, whereas there was no significant difference between the two cell lines.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2004.06.035</identifier><identifier>PMID: 15488635</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Antineoplastic Agents - metabolism ; Antineoplastic Agents - therapeutic use ; Bladder cancer ; Cancer therapies ; Cell Line ; Cell Line, Tumor ; Chemotherapy ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA, Neoplasm - metabolism ; Drug Screening Assays, Antitumor ; Hematoporphyrins - metabolism ; Hematoporphyrins - therapeutic use ; Humans ; Organoplatinum Compounds - chemistry ; Organoplatinum Compounds - metabolism ; Organoplatinum Compounds - therapeutic use ; Photodynamic therapy ; Photosensitizing Agents - metabolism ; Photosensitizing Agents - therapeutic use ; Platinum ; Platinum - metabolism ; Porphyrins - chemistry ; Porphyrins - therapeutic use ; Porphyrin–platinum complex ; Proteins ; Selective accumulation ; Spheroids, Cellular ; Tissue Distribution ; Tumors ; Urinary Bladder - metabolism ; Urinary Bladder Neoplasms - drug therapy ; Urinary Bladder Neoplasms - metabolism</subject><ispartof>Cancer letters, 2004-11, Vol.215 (2), p.167-177</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Nov 25, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-2010394b7bcbe231e5662258eda56893dd8593a8e441cfb5af419555f8d0f3de3</citedby><cites>FETCH-LOGICAL-c452t-2010394b7bcbe231e5662258eda56893dd8593a8e441cfb5af419555f8d0f3de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304383504004744$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15488635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lottner, Christian</creatorcontrib><creatorcontrib>Knuechel, Ruth</creatorcontrib><creatorcontrib>Bernhardt, Guenther</creatorcontrib><creatorcontrib>Brunner, Henri</creatorcontrib><title>Distribution and subcellular localization of a water-soluble hematoporphyrin–platinum(II) complex in human bladder cancer cells</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>The water-soluble porphyrin–platinum complex diammine{7,12-bis[1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato}platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates and degree of DNA platination on the low-differentiated J82 cells, a model of invasive bladder cancer, and UROtsa, a normal urothelial cell line. Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 cancer cells than in UROtsa cells. Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA platination in J82 and UROtsa cells was higher by PEG-HPPt than by cisplatin, whereas there was no significant difference between the two cell lines.</description><subject>Antineoplastic Agents - metabolism</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Bladder cancer</subject><subject>Cancer therapies</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Neoplasm - metabolism</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Hematoporphyrins - metabolism</subject><subject>Hematoporphyrins - therapeutic use</subject><subject>Humans</subject><subject>Organoplatinum Compounds - chemistry</subject><subject>Organoplatinum Compounds - metabolism</subject><subject>Organoplatinum Compounds - therapeutic use</subject><subject>Photodynamic therapy</subject><subject>Photosensitizing Agents - metabolism</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Platinum</subject><subject>Platinum - metabolism</subject><subject>Porphyrins - chemistry</subject><subject>Porphyrins - therapeutic use</subject><subject>Porphyrin–platinum complex</subject><subject>Proteins</subject><subject>Selective accumulation</subject><subject>Spheroids, Cellular</subject><subject>Tissue Distribution</subject><subject>Tumors</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary Bladder Neoplasms - drug therapy</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuqFDEQhoMonvHoG4gEBNFFj0nn0umNIMfbwAE3ug7ppJrJkO60SUc9rvQZfEOfxIwzILhwVYv6quqnPoQeUrKlhMrnh601c4B12xLCt0RuCRO30Iaqrm26XpHbaEMY4Q1TTFygezkfCCGCd-IuuqCCKyWZ2KAfr3xekx_K6uOMzexwLoOFEEowCYdoTfDfzJ9mHLHBX8wKqckxlCEA3sNk1rjEtOxvkp9_ff-5hArPZXq62z3DNk5LgK_Yz3hfJjPjIRjnIOGa3B5LvZPvozujCRkenOsl-vjm9Yerd831-7e7q5fXjeWiXZuWUMJ6PnSDHaBlFISUbSsUOCOk6plzSvTMKOCc2nEQZuS0F0KMypGROWCX6Mlp75LipwJ51ZPPxwRmhliylrJXQnVdBR__Ax5iSXPNpqkggklFelEpfqJsijknGPWS_GTSjaZEHwXpgz4J0kdBmkhdBdWxR-flZZjA_R06G6nAixMA9RefPSSdrYf6LucT2FW76P9_4TdNgKZx</recordid><startdate>20041125</startdate><enddate>20041125</enddate><creator>Lottner, Christian</creator><creator>Knuechel, Ruth</creator><creator>Bernhardt, Guenther</creator><creator>Brunner, Henri</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20041125</creationdate><title>Distribution and subcellular localization of a water-soluble hematoporphyrin–platinum(II) complex in human bladder cancer cells</title><author>Lottner, Christian ; Knuechel, Ruth ; Bernhardt, Guenther ; Brunner, Henri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-2010394b7bcbe231e5662258eda56893dd8593a8e441cfb5af419555f8d0f3de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antineoplastic Agents - metabolism</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Bladder cancer</topic><topic>Cancer therapies</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Chemotherapy</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Neoplasm - metabolism</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Hematoporphyrins - metabolism</topic><topic>Hematoporphyrins - therapeutic use</topic><topic>Humans</topic><topic>Organoplatinum Compounds - chemistry</topic><topic>Organoplatinum Compounds - metabolism</topic><topic>Organoplatinum Compounds - therapeutic use</topic><topic>Photodynamic therapy</topic><topic>Photosensitizing Agents - metabolism</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Platinum</topic><topic>Platinum - metabolism</topic><topic>Porphyrins - chemistry</topic><topic>Porphyrins - therapeutic use</topic><topic>Porphyrin–platinum complex</topic><topic>Proteins</topic><topic>Selective accumulation</topic><topic>Spheroids, Cellular</topic><topic>Tissue Distribution</topic><topic>Tumors</topic><topic>Urinary Bladder - metabolism</topic><topic>Urinary Bladder Neoplasms - drug therapy</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lottner, Christian</creatorcontrib><creatorcontrib>Knuechel, Ruth</creatorcontrib><creatorcontrib>Bernhardt, Guenther</creatorcontrib><creatorcontrib>Brunner, Henri</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lottner, Christian</au><au>Knuechel, Ruth</au><au>Bernhardt, Guenther</au><au>Brunner, Henri</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and subcellular localization of a water-soluble hematoporphyrin–platinum(II) complex in human bladder cancer cells</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2004-11-25</date><risdate>2004</risdate><volume>215</volume><issue>2</issue><spage>167</spage><epage>177</epage><pages>167-177</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>The water-soluble porphyrin–platinum complex diammine{7,12-bis[1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato}platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates and degree of DNA platination on the low-differentiated J82 cells, a model of invasive bladder cancer, and UROtsa, a normal urothelial cell line. 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| ispartof | Cancer letters, 2004-11, Vol.215 (2), p.167-177 |
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| subjects | Antineoplastic Agents - metabolism Antineoplastic Agents - therapeutic use Bladder cancer Cancer therapies Cell Line Cell Line, Tumor Chemotherapy Cytotoxicity Deoxyribonucleic acid DNA DNA, Neoplasm - metabolism Drug Screening Assays, Antitumor Hematoporphyrins - metabolism Hematoporphyrins - therapeutic use Humans Organoplatinum Compounds - chemistry Organoplatinum Compounds - metabolism Organoplatinum Compounds - therapeutic use Photodynamic therapy Photosensitizing Agents - metabolism Photosensitizing Agents - therapeutic use Platinum Platinum - metabolism Porphyrins - chemistry Porphyrins - therapeutic use Porphyrin–platinum complex Proteins Selective accumulation Spheroids, Cellular Tissue Distribution Tumors Urinary Bladder - metabolism Urinary Bladder Neoplasms - drug therapy Urinary Bladder Neoplasms - metabolism |
| title | Distribution and subcellular localization of a water-soluble hematoporphyrin–platinum(II) complex in human bladder cancer cells |
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