Improved Infrared Multiphoton Dissociation of Peptides through N-Terminal Phosphonite Derivatization

A strategy for improving the sequencing of peptides by infrared multiphoton dissociation (IRMPD) in a linear ion trap mass spectrometer is described. We have developed an N-terminal derivatization reagent, 4-methylphosphonophenylisothiocyanate (PPITC), which allows the attachment of an IR-chromogeni...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the American Society for Mass Spectrometry 2009-03, Vol.20 (3), p.377-384
Hauptverfasser:	Vasicek, Lisa A., Wilson, Jeffrey J., Brodbelt, Jennifer S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Analytical, structural and metabolic biochemistry Benzenesulfonates - chemistry Bioinformatics Biological and medical sciences Biotechnology Chemistry Chemistry and Materials Science Dissociation Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Infrared lasers Isothiocyanates - chemistry Mass spectra Mass spectrometry Mass Spectrometry - methods Organic Chemistry Organophosphonates - chemistry Peptides Peptides - chemistry Proteins Proteomics Reactivity and mechanisms Sequence Analysis, Protein - methods Sequences Thiocyanates - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!