Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. l...

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Veröffentlicht in:	Parasitology research (1987) 2004-10, Vol.94 (4), p.318-320
Hauptverfasser:	SPITALSKA, Eva, TORINA, Alessandra, CANNELLA, Vincenza, CARACAPPA, Santo, SPARAGANO, Olivier A. E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arachnid Vectors - parasitology Biological and medical sciences Cattle Cattle Diseases - parasitology Deoxyribonuclease HpaII - metabolism DNA, Protozoan - analysis DNA, Ribosomal - analysis Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Invertebrates Polymorphism, Restriction Fragment Length RNA, Ribosomal, 18S - genetics Sheep Diseases - parasitology Species Specificity Theileria - classification Theileria - genetics Theileria annulata - classification Theileria annulata - genetics Theileriasis - parasitology Ticks - parasitology
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