Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine
Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmi...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 2009-03, Vol.145 (3), p.275-278 |
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| container_end_page | 278 |
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| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 145 |
| creator | Kishimoto, Satoko Nakamura, Shingo Hattori, Hidemi Nakamura, Shin-ichiro Oonuma, Fumie Kanatani, Yasuhiro Tanaka, Yoshihiro Mori, Yasutaka Harada, Yasuji Tagawa, Masahiro Ishihara, Masayuki |
| description | Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line. |
| doi_str_mv | 10.1093/jb/mvn169 |
| format | Article |
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The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line.</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/jb/mvn169</identifier><identifier>PMID: 19074504</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Disaccharides - analysis ; enzyme-linked immunosorbent assay (ELISA) ; fragmin/protamine microparticles (F/P MPs) ; Heparin - chemistry ; Heparin - metabolism ; heparin-binding cytokine ; heparinoids ; human stem cell factor (SCF) ; Humans ; Protein Binding - drug effects ; Stem Cell Factor - metabolism ; Stem Cell Factor - pharmacology</subject><ispartof>Journal of biochemistry (Tokyo), 2009-03, Vol.145 (3), p.275-278</ispartof><rights>The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-6eb56c4a7a9e3c52152e1d0fa7d4b0656b990f692db4c2b64c8664d32116c69f3</citedby><cites>FETCH-LOGICAL-c466t-6eb56c4a7a9e3c52152e1d0fa7d4b0656b990f692db4c2b64c8664d32116c69f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19074504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kishimoto, Satoko</creatorcontrib><creatorcontrib>Nakamura, Shingo</creatorcontrib><creatorcontrib>Hattori, Hidemi</creatorcontrib><creatorcontrib>Nakamura, Shin-ichiro</creatorcontrib><creatorcontrib>Oonuma, Fumie</creatorcontrib><creatorcontrib>Kanatani, Yasuhiro</creatorcontrib><creatorcontrib>Tanaka, Yoshihiro</creatorcontrib><creatorcontrib>Mori, Yasutaka</creatorcontrib><creatorcontrib>Harada, Yasuji</creatorcontrib><creatorcontrib>Tagawa, Masahiro</creatorcontrib><creatorcontrib>Ishihara, Masayuki</creatorcontrib><title>Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line.</description><subject>Disaccharides - analysis</subject><subject>enzyme-linked immunosorbent assay (ELISA)</subject><subject>fragmin/protamine microparticles (F/P MPs)</subject><subject>Heparin - chemistry</subject><subject>Heparin - metabolism</subject><subject>heparin-binding cytokine</subject><subject>heparinoids</subject><subject>human stem cell factor (SCF)</subject><subject>Humans</subject><subject>Protein Binding - drug effects</subject><subject>Stem Cell Factor - metabolism</subject><subject>Stem Cell Factor - pharmacology</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E9LwzAYBvAgipvTg19AehBxh7okTd8u3lxxVhkqTGV4CWmaSrf1j0kr7tvb0aE3Ty8v_Hh4eBA6JfiKYO6NlvEo_yoI8D3UJ4EPLgWf7KM-xpS4nLJFDx1Zu9y-1PMOUY9wHDAfsz66jppcFs681rkT6vXamUpVl8a5nIfToZNZRzqRrqTJCneSFUlWfDjhpi5XWaGP0UEq11af7O4AvU5vX8LInT3d3Yc3M1cxgNoFHfugmAwk157yKfGpJglOZZCwGIMPMec4BU6TmCkaA1NjAJZ4lBBQwFNvgC663MqUn422tcgzq9qustBlYwUAH2OCxy0cdlCZ0lqjU1GZLJdmIwgW26HEMhbdUK0924U2ca6TP7lbpgXnHSib6t8ct2OZrfX3L5RmJSDwAl9Ei3fx_Pj28BDyiZh7P3NvfRA</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Kishimoto, Satoko</creator><creator>Nakamura, Shingo</creator><creator>Hattori, Hidemi</creator><creator>Nakamura, Shin-ichiro</creator><creator>Oonuma, Fumie</creator><creator>Kanatani, Yasuhiro</creator><creator>Tanaka, Yoshihiro</creator><creator>Mori, Yasutaka</creator><creator>Harada, Yasuji</creator><creator>Tagawa, Masahiro</creator><creator>Ishihara, Masayuki</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine</title><author>Kishimoto, Satoko ; Nakamura, Shingo ; Hattori, Hidemi ; Nakamura, Shin-ichiro ; Oonuma, Fumie ; Kanatani, Yasuhiro ; Tanaka, Yoshihiro ; Mori, Yasutaka ; Harada, Yasuji ; Tagawa, Masahiro ; Ishihara, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-6eb56c4a7a9e3c52152e1d0fa7d4b0656b990f692db4c2b64c8664d32116c69f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Disaccharides - analysis</topic><topic>enzyme-linked immunosorbent assay (ELISA)</topic><topic>fragmin/protamine microparticles (F/P MPs)</topic><topic>Heparin - chemistry</topic><topic>Heparin - metabolism</topic><topic>heparin-binding cytokine</topic><topic>heparinoids</topic><topic>human stem cell factor (SCF)</topic><topic>Humans</topic><topic>Protein Binding - drug effects</topic><topic>Stem Cell Factor - metabolism</topic><topic>Stem Cell Factor - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishimoto, Satoko</creatorcontrib><creatorcontrib>Nakamura, Shingo</creatorcontrib><creatorcontrib>Hattori, Hidemi</creatorcontrib><creatorcontrib>Nakamura, Shin-ichiro</creatorcontrib><creatorcontrib>Oonuma, Fumie</creatorcontrib><creatorcontrib>Kanatani, Yasuhiro</creatorcontrib><creatorcontrib>Tanaka, Yoshihiro</creatorcontrib><creatorcontrib>Mori, Yasutaka</creatorcontrib><creatorcontrib>Harada, Yasuji</creatorcontrib><creatorcontrib>Tagawa, Masahiro</creatorcontrib><creatorcontrib>Ishihara, Masayuki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishimoto, Satoko</au><au>Nakamura, Shingo</au><au>Hattori, Hidemi</au><au>Nakamura, Shin-ichiro</au><au>Oonuma, Fumie</au><au>Kanatani, Yasuhiro</au><au>Tanaka, Yoshihiro</au><au>Mori, Yasutaka</au><au>Harada, Yasuji</au><au>Tagawa, Masahiro</au><au>Ishihara, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>145</volume><issue>3</issue><spage>275</spage><epage>278</epage><pages>275-278</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><abstract>Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). 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| fulltext | fulltext |
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| ispartof | Journal of biochemistry (Tokyo), 2009-03, Vol.145 (3), p.275-278 |
| issn | 0021-924X 1756-2651 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Disaccharides - analysis enzyme-linked immunosorbent assay (ELISA) fragmin/protamine microparticles (F/P MPs) Heparin - chemistry Heparin - metabolism heparin-binding cytokine heparinoids human stem cell factor (SCF) Humans Protein Binding - drug effects Stem Cell Factor - metabolism Stem Cell Factor - pharmacology |
| title | Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine |
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