Effect of pH on the stability of Pleurotus eryngii versatile peroxidase during heterologous production in Emericella nidulans

Complementary DNA (cDNA) encoding the new versatile peroxidase from the ligninolytic basidiomycete Pleurotus eryngii has been expressed in the ascomycete Emericella nidulans. In recombinant E. nidulans cultures, the pH reached values as high as 8.3, correlating with a sharp decrease in peroxidase ac...

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Veröffentlicht in:	Bioprocess and biosystems engineering 2004-10, Vol.26 (5), p.287-293
Hauptverfasser:	Lú-Chau, T A, Ruiz-Dueñas, F J, Camarero, S, Feijoo, G, Martínez, M J, Lema, J M, Martínez, A T
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors Bioreactors - microbiology Cell Culture Techniques - methods Deoxyribonucleic acid DNA Emericella - enzymology Emericella - genetics Emericella nidulans Enzyme Activation Enzyme Stability Gene expression Hydrogen-Ion Concentration Inactivation Peroxidase - biosynthesis Peroxidase - chemistry Peroxidase - genetics Pleurotus - enzymology Pleurotus - genetics Pleurotus eryngii Protein Engineering - methods Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Temperature effects
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