Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus
Abstract Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells...
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| Veröffentlicht in: | Brain research 2009-03, Vol.1258, p.34-42 |
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| creator | Ohbuchi, Toyoaki Yokoyama, Toru Saito, Takeshi Hashimoto, Hirofumi Suzuki, Hitoshi Otsubo, Hiroki Fujihara, Hiroaki Suzuki, Hideaki Ueta, Yoichi |
| description | Abstract Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis. |
| doi_str_mv | 10.1016/j.brainres.2008.12.057 |
| format | Article |
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The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2008.12.057</identifier><identifier>PMID: 19150437</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - metabolism ; Carbazoles - pharmacology ; Central nervous system ; Electrophysiology ; Enzyme Inhibitors - pharmacology ; Excitatory Postsynaptic Potentials - physiology ; Fundamental and applied biological sciences. Psychology ; GABA ; gamma-Aminobutyric Acid - metabolism ; Green fluorescent protein ; Green Fluorescent Proteins - genetics ; In Vitro Techniques ; Indole Alkaloids - pharmacology ; Inhibitory Postsynaptic Potentials - physiology ; Male ; Neurology ; Neurons - physiology ; Neurosecretion ; Patch-Clamp Techniques ; Rats ; Rats, Transgenic ; Rats, Wistar ; Receptor, trkB - antagonists & inhibitors ; Receptor, trkB - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Supraoptic nucleus ; Supraoptic Nucleus - physiology ; Vertebrates: nervous system and sense organs ; Whole-cell patch-clamp</subject><ispartof>Brain research, 2009-03, Vol.1258, p.34-42</ispartof><rights>Elsevier B.V.</rights><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-1b882f777a0fbe37e02c0e92de985882738a5b11048d99d211048ac5edb520423</citedby><cites>FETCH-LOGICAL-c548t-1b882f777a0fbe37e02c0e92de985882738a5b11048d99d211048ac5edb520423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2008.12.057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21242974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19150437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohbuchi, Toyoaki</creatorcontrib><creatorcontrib>Yokoyama, Toru</creatorcontrib><creatorcontrib>Saito, Takeshi</creatorcontrib><creatorcontrib>Hashimoto, Hirofumi</creatorcontrib><creatorcontrib>Suzuki, Hitoshi</creatorcontrib><creatorcontrib>Otsubo, Hiroki</creatorcontrib><creatorcontrib>Fujihara, Hiroaki</creatorcontrib><creatorcontrib>Suzuki, Hideaki</creatorcontrib><creatorcontrib>Ueta, Yoichi</creatorcontrib><title>Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Carbazoles - pharmacology</subject><subject>Central nervous system</subject><subject>Electrophysiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GABA</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>In Vitro Techniques</subject><subject>Indole Alkaloids - pharmacology</subject><subject>Inhibitory Postsynaptic Potentials - physiology</subject><subject>Male</subject><subject>Neurology</subject><subject>Neurons - physiology</subject><subject>Neurosecretion</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><subject>Rats, Transgenic</subject><subject>Rats, Wistar</subject><subject>Receptor, trkB - antagonists & inhibitors</subject><subject>Receptor, trkB - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Supraoptic nucleus</subject><subject>Supraoptic Nucleus - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Whole-cell patch-clamp</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi0EokvhL1S5wC3L2E5i-4KgFV9SJQ7A2XKcidZL1g62U2n_PU53CxKXnvwxzztjzzuEXFHYUqDd2_22j8b5iGnLAOSWsi204gnZUClY3bEGnpINAHS1VIpfkBcp7cuRcwXPyQVVtIWGiw1Zrtc09YDR3eFQeVxiyDHMO2er0dgcYuX8zvUupyrNwWfjMSzp4TLEYzWHlNPRmzkXjV1iRJ9XoMo7rKLJVVrmaMJ92C92wiW9JM9GMyV8dV4vyc9PH3_cfKlvv33-evPhtrZtI3NNeynZKIQwMPbIBQKzgIoNqGRbQoJL0_aUQiMHpQZ2vzO2xaFvGTSMX5I3p7xzDL8XTFkfXLI4Tadf6K5TojSifRRkwDtV-lzA7gTaGFKKOOo5uoOJR01Br87ovX5wRq_OaMp0caYIr84Vlv6Awz_Z2YoCvD4DJlkzjdF469JfjlHWMCWawr0_cVgad-cw6mQdeouDi2izHoJ7_C3v_kthJ-ddqfoLj5j2YYm-2KKpTkWgv69ztI4RSOCgmOR_AC6Qx08</recordid><startdate>20090303</startdate><enddate>20090303</enddate><creator>Ohbuchi, Toyoaki</creator><creator>Yokoyama, Toru</creator><creator>Saito, Takeshi</creator><creator>Hashimoto, Hirofumi</creator><creator>Suzuki, Hitoshi</creator><creator>Otsubo, Hiroki</creator><creator>Fujihara, Hiroaki</creator><creator>Suzuki, Hideaki</creator><creator>Ueta, Yoichi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20090303</creationdate><title>Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus</title><author>Ohbuchi, Toyoaki ; Yokoyama, Toru ; Saito, Takeshi ; Hashimoto, Hirofumi ; Suzuki, Hitoshi ; Otsubo, Hiroki ; Fujihara, Hiroaki ; Suzuki, Hideaki ; Ueta, Yoichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-1b882f777a0fbe37e02c0e92de985882738a5b11048d99d211048ac5edb520423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Carbazoles - pharmacology</topic><topic>Central nervous system</topic><topic>Electrophysiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GABA</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>In Vitro Techniques</topic><topic>Indole Alkaloids - pharmacology</topic><topic>Inhibitory Postsynaptic Potentials - physiology</topic><topic>Male</topic><topic>Neurology</topic><topic>Neurons - physiology</topic><topic>Neurosecretion</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><topic>Rats, Transgenic</topic><topic>Rats, Wistar</topic><topic>Receptor, trkB - antagonists & inhibitors</topic><topic>Receptor, trkB - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Supraoptic nucleus</topic><topic>Supraoptic Nucleus - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Whole-cell patch-clamp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohbuchi, Toyoaki</creatorcontrib><creatorcontrib>Yokoyama, Toru</creatorcontrib><creatorcontrib>Saito, Takeshi</creatorcontrib><creatorcontrib>Hashimoto, Hirofumi</creatorcontrib><creatorcontrib>Suzuki, Hitoshi</creatorcontrib><creatorcontrib>Otsubo, Hiroki</creatorcontrib><creatorcontrib>Fujihara, Hiroaki</creatorcontrib><creatorcontrib>Suzuki, Hideaki</creatorcontrib><creatorcontrib>Ueta, Yoichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohbuchi, Toyoaki</au><au>Yokoyama, Toru</au><au>Saito, Takeshi</au><au>Hashimoto, Hirofumi</au><au>Suzuki, Hitoshi</au><au>Otsubo, Hiroki</au><au>Fujihara, Hiroaki</au><au>Suzuki, Hideaki</au><au>Ueta, Yoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2009-03-03</date><risdate>2009</risdate><volume>1258</volume><spage>34</spage><epage>42</epage><pages>34-42</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Abstract Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19150437</pmid><doi>10.1016/j.brainres.2008.12.057</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - metabolism Carbazoles - pharmacology Central nervous system Electrophysiology Enzyme Inhibitors - pharmacology Excitatory Postsynaptic Potentials - physiology Fundamental and applied biological sciences. Psychology GABA gamma-Aminobutyric Acid - metabolism Green fluorescent protein Green Fluorescent Proteins - genetics In Vitro Techniques Indole Alkaloids - pharmacology Inhibitory Postsynaptic Potentials - physiology Male Neurology Neurons - physiology Neurosecretion Patch-Clamp Techniques Rats Rats, Transgenic Rats, Wistar Receptor, trkB - antagonists & inhibitors Receptor, trkB - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Supraoptic nucleus Supraoptic Nucleus - physiology Vertebrates: nervous system and sense organs Whole-cell patch-clamp |
| title | Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus |
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