Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans
Abstract Objectives This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococc...
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| Veröffentlicht in: | Journal of dentistry 2009-04, Vol.37 (4), p.289-296 |
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| creator | Li, Fang Chen, Jihua Chai, Zhiguo Zhang, Ling Xiao, Yuhong Fang, Ming Ma, Sai |
| description | Abstract Objectives This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococcus mutans. Methods DMAE-CB was incorporated at 3% (w/v) into a clinically used dental adhesive, Single Bond 2. Single Bond 2 without DMAE-CB was served as a negative control; Clearfil Protect Bond, containing an intensively researched antibacterial monomer MDPB, was enrolled as a positive control. The effects of the cured adhesives and their eluents on the growth of S. mutans were determined by film contact test and absorbance measurement, respectively. The effects of the cured adhesives on the adherence and membrane integrity of S. mutans were investigated using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent indicators. Results Compared with negative control, the cured DMAE-CB-incorporated dental adhesive and positive control were found to exhibit inhibitory effect on the growth of S. mutans ( P < 0.05), whereas their eluents did not show detectable antibacterial activity. Moreover, the fluorescence analysis of CLSM images demonstrated that the cured DMAE-CB-incorporated adhesive and positive control could hamper the adherence of S. mutans and exert detrimental effect on bacterial membrane integrity ( P < 0.05). Conclusions The incorporation of DMAE-CB can render dental adhesive with contact antibacterial activity after polymerization via influencing the growth, adherence and membrane integrity of S. mutans. |
| doi_str_mv | 10.1016/j.jdent.2008.12.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66971232</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0300571208003448</els_id><sourcerecordid>2721427871</sourcerecordid><originalsourceid>FETCH-LOGICAL-c506t-f113ddfb26e0e9b2d12fd508ecda03586844a1c621edfbc554d12a64d8b9f6ec3</originalsourceid><addsrcrecordid>eNqFks2KFDEUhYMoTjv6BIIEBFd2mZ-qdGqhIMM4Iwy4GAV3IZXc6k5ZlbRJaqTfwMc2Nd0wMBtX2Xzn3HvPCUKvKakooeLDUA0WfK4YIbKirCKkfoJWVG7aNd2In0_RinBC1s2GsjP0IqWBFIKw9jk6oy2VTU3kCv297HswOeHQY40XPz1ibXeQ3B1g502I-xB1dn6Ltc-u0yZDdAWagg8TRBw8zjvA2xj-5N37e20Eb6DgFk8wdVH7xSnDNrp8WAbd5gj7HEwwZk54mrP26SV61usxwavTe45-fLn8fnG9vvl29fXi883aNETkdU8pt7bvmAACbccsZb1tiARjNeGNFLKuNTWCUSiUaZq6EFrUVnZtL8Dwc_Tu6LuP4fcMKavJJQPjWLYMc1JCtCUwzgr49hE4hDn6spuiS7CSbzgpFD9SJoaUIvRqH92k46FAaqlJDeq-JrXUpChTpYSienPynrsJ7IPm1EsBPh4BKFHcOYgqGbekal0sdSkb3H8GfHqkN6PzzujxFxwgPVyiUhGo2-Wg5aMQSQiva8n_ARNevGY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1030083730</pqid></control><display><type>article</type><title>Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Li, Fang ; Chen, Jihua ; Chai, Zhiguo ; Zhang, Ling ; Xiao, Yuhong ; Fang, Ming ; Ma, Sai</creator><creatorcontrib>Li, Fang ; Chen, Jihua ; Chai, Zhiguo ; Zhang, Ling ; Xiao, Yuhong ; Fang, Ming ; Ma, Sai</creatorcontrib><description>Abstract Objectives This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococcus mutans. Methods DMAE-CB was incorporated at 3% (w/v) into a clinically used dental adhesive, Single Bond 2. Single Bond 2 without DMAE-CB was served as a negative control; Clearfil Protect Bond, containing an intensively researched antibacterial monomer MDPB, was enrolled as a positive control. The effects of the cured adhesives and their eluents on the growth of S. mutans were determined by film contact test and absorbance measurement, respectively. The effects of the cured adhesives on the adherence and membrane integrity of S. mutans were investigated using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent indicators. Results Compared with negative control, the cured DMAE-CB-incorporated dental adhesive and positive control were found to exhibit inhibitory effect on the growth of S. mutans ( P < 0.05), whereas their eluents did not show detectable antibacterial activity. Moreover, the fluorescence analysis of CLSM images demonstrated that the cured DMAE-CB-incorporated adhesive and positive control could hamper the adherence of S. mutans and exert detrimental effect on bacterial membrane integrity ( P < 0.05). Conclusions The incorporation of DMAE-CB can render dental adhesive with contact antibacterial activity after polymerization via influencing the growth, adherence and membrane integrity of S. mutans.</description><identifier>ISSN: 0300-5712</identifier><identifier>EISSN: 1879-176X</identifier><identifier>DOI: 10.1016/j.jdent.2008.12.004</identifier><identifier>PMID: 19185408</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adhesives ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - therapeutic use ; Antibacterial ; Antimicrobial agents ; Bacteria ; Bacterial Adhesion - drug effects ; Biofilms - drug effects ; Bisphenol A-Glycidyl Methacrylate - chemistry ; Bisphenol A-Glycidyl Methacrylate - therapeutic use ; Bond strength ; Cell Membrane Permeability - drug effects ; Chemical bonds ; Colony Count, Microbial ; Confocal microscopy ; Dental adhesive ; Dental Materials - chemistry ; Dental Materials - therapeutic use ; Dentin-Bonding Agents - chemistry ; Dentin-Bonding Agents - therapeutic use ; Dentistry ; Efficiency ; Fluorides ; Methacrylates - chemistry ; Methacrylates - therapeutic use ; Microbiology ; Quaternary ammonium ; Quaternary Ammonium Compounds - chemistry ; Quaternary Ammonium Compounds - therapeutic use ; Streptococcus mutans - drug effects ; Streptococcus mutans - physiology ; Viable staining</subject><ispartof>Journal of dentistry, 2009-04, Vol.37 (4), p.289-296</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-f113ddfb26e0e9b2d12fd508ecda03586844a1c621edfbc554d12a64d8b9f6ec3</citedby><cites>FETCH-LOGICAL-c506t-f113ddfb26e0e9b2d12fd508ecda03586844a1c621edfbc554d12a64d8b9f6ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300571208003448$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19185408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Chen, Jihua</creatorcontrib><creatorcontrib>Chai, Zhiguo</creatorcontrib><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Xiao, Yuhong</creatorcontrib><creatorcontrib>Fang, Ming</creatorcontrib><creatorcontrib>Ma, Sai</creatorcontrib><title>Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans</title><title>Journal of dentistry</title><addtitle>J Dent</addtitle><description>Abstract Objectives This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococcus mutans. Methods DMAE-CB was incorporated at 3% (w/v) into a clinically used dental adhesive, Single Bond 2. Single Bond 2 without DMAE-CB was served as a negative control; Clearfil Protect Bond, containing an intensively researched antibacterial monomer MDPB, was enrolled as a positive control. The effects of the cured adhesives and their eluents on the growth of S. mutans were determined by film contact test and absorbance measurement, respectively. The effects of the cured adhesives on the adherence and membrane integrity of S. mutans were investigated using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent indicators. Results Compared with negative control, the cured DMAE-CB-incorporated dental adhesive and positive control were found to exhibit inhibitory effect on the growth of S. mutans ( P < 0.05), whereas their eluents did not show detectable antibacterial activity. Moreover, the fluorescence analysis of CLSM images demonstrated that the cured DMAE-CB-incorporated adhesive and positive control could hamper the adherence of S. mutans and exert detrimental effect on bacterial membrane integrity ( P < 0.05). Conclusions The incorporation of DMAE-CB can render dental adhesive with contact antibacterial activity after polymerization via influencing the growth, adherence and membrane integrity of S. mutans.</description><subject>Adhesives</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibacterial</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Biofilms - drug effects</subject><subject>Bisphenol A-Glycidyl Methacrylate - chemistry</subject><subject>Bisphenol A-Glycidyl Methacrylate - therapeutic use</subject><subject>Bond strength</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Chemical bonds</subject><subject>Colony Count, Microbial</subject><subject>Confocal microscopy</subject><subject>Dental adhesive</subject><subject>Dental Materials - chemistry</subject><subject>Dental Materials - therapeutic use</subject><subject>Dentin-Bonding Agents - chemistry</subject><subject>Dentin-Bonding Agents - therapeutic use</subject><subject>Dentistry</subject><subject>Efficiency</subject><subject>Fluorides</subject><subject>Methacrylates - chemistry</subject><subject>Methacrylates - therapeutic use</subject><subject>Microbiology</subject><subject>Quaternary ammonium</subject><subject>Quaternary Ammonium Compounds - chemistry</subject><subject>Quaternary Ammonium Compounds - therapeutic use</subject><subject>Streptococcus mutans - drug effects</subject><subject>Streptococcus mutans - physiology</subject><subject>Viable staining</subject><issn>0300-5712</issn><issn>1879-176X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2KFDEUhYMoTjv6BIIEBFd2mZ-qdGqhIMM4Iwy4GAV3IZXc6k5ZlbRJaqTfwMc2Nd0wMBtX2Xzn3HvPCUKvKakooeLDUA0WfK4YIbKirCKkfoJWVG7aNd2In0_RinBC1s2GsjP0IqWBFIKw9jk6oy2VTU3kCv297HswOeHQY40XPz1ibXeQ3B1g502I-xB1dn6Ltc-u0yZDdAWagg8TRBw8zjvA2xj-5N37e20Eb6DgFk8wdVH7xSnDNrp8WAbd5gj7HEwwZk54mrP26SV61usxwavTe45-fLn8fnG9vvl29fXi883aNETkdU8pt7bvmAACbccsZb1tiARjNeGNFLKuNTWCUSiUaZq6EFrUVnZtL8Dwc_Tu6LuP4fcMKavJJQPjWLYMc1JCtCUwzgr49hE4hDn6spuiS7CSbzgpFD9SJoaUIvRqH92k46FAaqlJDeq-JrXUpChTpYSienPynrsJ7IPm1EsBPh4BKFHcOYgqGbekal0sdSkb3H8GfHqkN6PzzujxFxwgPVyiUhGo2-Wg5aMQSQiva8n_ARNevGY</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Li, Fang</creator><creator>Chen, Jihua</creator><creator>Chai, Zhiguo</creator><creator>Zhang, Ling</creator><creator>Xiao, Yuhong</creator><creator>Fang, Ming</creator><creator>Ma, Sai</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans</title><author>Li, Fang ; Chen, Jihua ; Chai, Zhiguo ; Zhang, Ling ; Xiao, Yuhong ; Fang, Ming ; Ma, Sai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-f113ddfb26e0e9b2d12fd508ecda03586844a1c621edfbc554d12a64d8b9f6ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adhesives</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antibacterial</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Biofilms - drug effects</topic><topic>Bisphenol A-Glycidyl Methacrylate - chemistry</topic><topic>Bisphenol A-Glycidyl Methacrylate - therapeutic use</topic><topic>Bond strength</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Chemical bonds</topic><topic>Colony Count, Microbial</topic><topic>Confocal microscopy</topic><topic>Dental adhesive</topic><topic>Dental Materials - chemistry</topic><topic>Dental Materials - therapeutic use</topic><topic>Dentin-Bonding Agents - chemistry</topic><topic>Dentin-Bonding Agents - therapeutic use</topic><topic>Dentistry</topic><topic>Efficiency</topic><topic>Fluorides</topic><topic>Methacrylates - chemistry</topic><topic>Methacrylates - therapeutic use</topic><topic>Microbiology</topic><topic>Quaternary ammonium</topic><topic>Quaternary Ammonium Compounds - chemistry</topic><topic>Quaternary Ammonium Compounds - therapeutic use</topic><topic>Streptococcus mutans - drug effects</topic><topic>Streptococcus mutans - physiology</topic><topic>Viable staining</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Chen, Jihua</creatorcontrib><creatorcontrib>Chai, Zhiguo</creatorcontrib><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Xiao, Yuhong</creatorcontrib><creatorcontrib>Fang, Ming</creatorcontrib><creatorcontrib>Ma, Sai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Fang</au><au>Chen, Jihua</au><au>Chai, Zhiguo</au><au>Zhang, Ling</au><au>Xiao, Yuhong</au><au>Fang, Ming</au><au>Ma, Sai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans</atitle><jtitle>Journal of dentistry</jtitle><addtitle>J Dent</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>37</volume><issue>4</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0300-5712</issn><eissn>1879-176X</eissn><abstract>Abstract Objectives This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococcus mutans. Methods DMAE-CB was incorporated at 3% (w/v) into a clinically used dental adhesive, Single Bond 2. Single Bond 2 without DMAE-CB was served as a negative control; Clearfil Protect Bond, containing an intensively researched antibacterial monomer MDPB, was enrolled as a positive control. The effects of the cured adhesives and their eluents on the growth of S. mutans were determined by film contact test and absorbance measurement, respectively. The effects of the cured adhesives on the adherence and membrane integrity of S. mutans were investigated using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent indicators. Results Compared with negative control, the cured DMAE-CB-incorporated dental adhesive and positive control were found to exhibit inhibitory effect on the growth of S. mutans ( P < 0.05), whereas their eluents did not show detectable antibacterial activity. Moreover, the fluorescence analysis of CLSM images demonstrated that the cured DMAE-CB-incorporated adhesive and positive control could hamper the adherence of S. mutans and exert detrimental effect on bacterial membrane integrity ( P < 0.05). Conclusions The incorporation of DMAE-CB can render dental adhesive with contact antibacterial activity after polymerization via influencing the growth, adherence and membrane integrity of S. mutans.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>19185408</pmid><doi>10.1016/j.jdent.2008.12.004</doi><tpages>8</tpages></addata></record> |
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| subjects | Adhesives Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - therapeutic use Antibacterial Antimicrobial agents Bacteria Bacterial Adhesion - drug effects Biofilms - drug effects Bisphenol A-Glycidyl Methacrylate - chemistry Bisphenol A-Glycidyl Methacrylate - therapeutic use Bond strength Cell Membrane Permeability - drug effects Chemical bonds Colony Count, Microbial Confocal microscopy Dental adhesive Dental Materials - chemistry Dental Materials - therapeutic use Dentin-Bonding Agents - chemistry Dentin-Bonding Agents - therapeutic use Dentistry Efficiency Fluorides Methacrylates - chemistry Methacrylates - therapeutic use Microbiology Quaternary ammonium Quaternary Ammonium Compounds - chemistry Quaternary Ammonium Compounds - therapeutic use Streptococcus mutans - drug effects Streptococcus mutans - physiology Viable staining |
| title | Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans |
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