Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry
Increased levels of modified nucleosides have been observed in urine from patients suffering from several cancers. In this study, we evaluated whether urinary nucleosides can serve as potential tumor markers for colorectal cancer by high performance liquid chromatography–electrospray/tandem mass spe...
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| Veröffentlicht in: | Clinica chimica acta 2009-04, Vol.402 (1), p.31-37 |
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| container_title | Clinica chimica acta |
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| creator | Hsu, Wei-Yi Chen, William Tzu-Liang Lin, Wei-De Tsai, Fuu-Jen Tsai, Yuhsin Lin, Ching-Tsung Lo, Wan-Yu Jeng, Long-Bin Lai, Chien-Chen |
| description | Increased levels of modified nucleosides have been observed in urine from patients suffering from several cancers. In this study, we evaluated whether urinary nucleosides can serve as potential tumor markers for colorectal cancer by high performance liquid chromatography–electrospray/tandem mass spectrometry (HPLC/ESI-MS/MS).
A simple and specific method based on HPLC/ESI-MS/MS was developed to determine the urinary nucleosides from patients with colorectal cancer. We studied the excretion patterns of nucleosides in urine from 26 patients with colorectal cancer and 18 healthy controls.
The LC/MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples with colorectal cancer. The mean levels of 5 urinary nucleosides (adenosine, cytidine, N
2,N
2-dimethylguanine, 8-hydroxy-2′-deoxyguanosine and uridine) were significantly higher in the patients with colorectal cancer than in the healthy adults.
The results indicate that urinary nucleosides determined by LC/MS/MS may be useful as biological markers for colorectal cancer. Our findings suggest that LC/MS/MS is a highly specific and sensitive method for rapidly screening a large number of nucleoside that may be useful as markers for cancer in humans. |
| doi_str_mv | 10.1016/j.cca.2008.12.009 |
| format | Article |
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A simple and specific method based on HPLC/ESI-MS/MS was developed to determine the urinary nucleosides from patients with colorectal cancer. We studied the excretion patterns of nucleosides in urine from 26 patients with colorectal cancer and 18 healthy controls.
The LC/MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples with colorectal cancer. The mean levels of 5 urinary nucleosides (adenosine, cytidine, N
2,N
2-dimethylguanine, 8-hydroxy-2′-deoxyguanosine and uridine) were significantly higher in the patients with colorectal cancer than in the healthy adults.
The results indicate that urinary nucleosides determined by LC/MS/MS may be useful as biological markers for colorectal cancer. Our findings suggest that LC/MS/MS is a highly specific and sensitive method for rapidly screening a large number of nucleoside that may be useful as markers for cancer in humans.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cca.2008.12.009</identifier><identifier>PMID: 19135043</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - urine ; Chromatography, High Pressure Liquid ; Colorectal cancer ; Colorectal Neoplasms - urine ; Female ; HPLC/MS/MS ; Humans ; Male ; Middle Aged ; Nucleosides ; Nucleosides - urine ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Tumor markers ; Urine</subject><ispartof>Clinica chimica acta, 2009-04, Vol.402 (1), p.31-37</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e2783a9dcb1923979ac1cab8f3580598c96624a9abbb4b5d2e3e5835222842093</citedby><cites>FETCH-LOGICAL-c417t-e2783a9dcb1923979ac1cab8f3580598c96624a9abbb4b5d2e3e5835222842093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009898108005950$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19135043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsu, Wei-Yi</creatorcontrib><creatorcontrib>Chen, William Tzu-Liang</creatorcontrib><creatorcontrib>Lin, Wei-De</creatorcontrib><creatorcontrib>Tsai, Fuu-Jen</creatorcontrib><creatorcontrib>Tsai, Yuhsin</creatorcontrib><creatorcontrib>Lin, Ching-Tsung</creatorcontrib><creatorcontrib>Lo, Wan-Yu</creatorcontrib><creatorcontrib>Jeng, Long-Bin</creatorcontrib><creatorcontrib>Lai, Chien-Chen</creatorcontrib><title>Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Increased levels of modified nucleosides have been observed in urine from patients suffering from several cancers. In this study, we evaluated whether urinary nucleosides can serve as potential tumor markers for colorectal cancer by high performance liquid chromatography–electrospray/tandem mass spectrometry (HPLC/ESI-MS/MS).
A simple and specific method based on HPLC/ESI-MS/MS was developed to determine the urinary nucleosides from patients with colorectal cancer. We studied the excretion patterns of nucleosides in urine from 26 patients with colorectal cancer and 18 healthy controls.
The LC/MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples with colorectal cancer. The mean levels of 5 urinary nucleosides (adenosine, cytidine, N
2,N
2-dimethylguanine, 8-hydroxy-2′-deoxyguanosine and uridine) were significantly higher in the patients with colorectal cancer than in the healthy adults.
The results indicate that urinary nucleosides determined by LC/MS/MS may be useful as biological markers for colorectal cancer. Our findings suggest that LC/MS/MS is a highly specific and sensitive method for rapidly screening a large number of nucleoside that may be useful as markers for cancer in humans.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - urine</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - urine</subject><subject>Female</subject><subject>HPLC/MS/MS</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nucleosides</subject><subject>Nucleosides - urine</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><subject>Tumor markers</subject><subject>Urine</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRS0EYpqBD2CDvGKXjB952GI1GvGSRmIDa6viVCZukjhjO0jhq_hE3NMtsWNVqtKpW6p7CXnLWckZb26OpbVQCsZUyUXJmH5GDly1spCVFs_JgeVRobTiV-RVjMfcVqzhL8kV11zWrJIH8ud2gWmPLlI_0C24BcJOl81O6KPrMVKIdPUJl-RgommbfaAzhJ8YInULHbcZFmr95APalAkLi8VAu52O7mGkK4bBh_k0pJN73FxP7Rj8DMk_BFjH_QanvBh8XAPs1PnF_YaUC02w9DjnWzHSuD4xM6awvyYvBpgivrnUa_Lj08fvd1-K-2-fv97d3he24m0qULRKgu5tx7WQutVguYVODbJWrNbK6qYRFWjouq7q6l6gxFrJWgihKsG0vCbvz7pr8I8bxmRmFy1OEyzot2iaRrfZzjaD_Aza_EUMOJg1uGzRbjgzp5jM0eSYzCkmw4VhT-LvLuJbN2P_b-OSSwY-nAHML_5yGEy0DrOLvTsZbXrv_iP_FxdFqJg</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Hsu, Wei-Yi</creator><creator>Chen, William Tzu-Liang</creator><creator>Lin, Wei-De</creator><creator>Tsai, Fuu-Jen</creator><creator>Tsai, Yuhsin</creator><creator>Lin, Ching-Tsung</creator><creator>Lo, Wan-Yu</creator><creator>Jeng, Long-Bin</creator><creator>Lai, Chien-Chen</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry</title><author>Hsu, Wei-Yi ; Chen, William Tzu-Liang ; Lin, Wei-De ; Tsai, Fuu-Jen ; Tsai, Yuhsin ; Lin, Ching-Tsung ; Lo, Wan-Yu ; Jeng, Long-Bin ; Lai, Chien-Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e2783a9dcb1923979ac1cab8f3580598c96624a9abbb4b5d2e3e5835222842093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - urine</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - urine</topic><topic>Female</topic><topic>HPLC/MS/MS</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nucleosides</topic><topic>Nucleosides - urine</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><topic>Tumor markers</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Wei-Yi</creatorcontrib><creatorcontrib>Chen, William Tzu-Liang</creatorcontrib><creatorcontrib>Lin, Wei-De</creatorcontrib><creatorcontrib>Tsai, Fuu-Jen</creatorcontrib><creatorcontrib>Tsai, Yuhsin</creatorcontrib><creatorcontrib>Lin, Ching-Tsung</creatorcontrib><creatorcontrib>Lo, Wan-Yu</creatorcontrib><creatorcontrib>Jeng, Long-Bin</creatorcontrib><creatorcontrib>Lai, Chien-Chen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Wei-Yi</au><au>Chen, William Tzu-Liang</au><au>Lin, Wei-De</au><au>Tsai, Fuu-Jen</au><au>Tsai, Yuhsin</au><au>Lin, Ching-Tsung</au><au>Lo, Wan-Yu</au><au>Jeng, Long-Bin</au><au>Lai, Chien-Chen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>402</volume><issue>1</issue><spage>31</spage><epage>37</epage><pages>31-37</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Increased levels of modified nucleosides have been observed in urine from patients suffering from several cancers. In this study, we evaluated whether urinary nucleosides can serve as potential tumor markers for colorectal cancer by high performance liquid chromatography–electrospray/tandem mass spectrometry (HPLC/ESI-MS/MS).
A simple and specific method based on HPLC/ESI-MS/MS was developed to determine the urinary nucleosides from patients with colorectal cancer. We studied the excretion patterns of nucleosides in urine from 26 patients with colorectal cancer and 18 healthy controls.
The LC/MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples with colorectal cancer. The mean levels of 5 urinary nucleosides (adenosine, cytidine, N
2,N
2-dimethylguanine, 8-hydroxy-2′-deoxyguanosine and uridine) were significantly higher in the patients with colorectal cancer than in the healthy adults.
The results indicate that urinary nucleosides determined by LC/MS/MS may be useful as biological markers for colorectal cancer. Our findings suggest that LC/MS/MS is a highly specific and sensitive method for rapidly screening a large number of nucleoside that may be useful as markers for cancer in humans.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19135043</pmid><doi>10.1016/j.cca.2008.12.009</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Biomarkers, Tumor - urine Chromatography, High Pressure Liquid Colorectal cancer Colorectal Neoplasms - urine Female HPLC/MS/MS Humans Male Middle Aged Nucleosides Nucleosides - urine Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Tumor markers Urine |
| title | Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry |
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