Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin
We present molecular and protein profiling of all acetylcholine receptors (ACh-R) in human scalp skin using PCR, in situ hybridization and double-labeling immunofluorescence. Within the epidermis, the nicotinic (n)ACh-R subunits, α3, α5, β2, and β4 were expressed in the basal cell layer (BCL) and in...
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description | We present molecular and protein profiling of all acetylcholine receptors (ACh-R) in human scalp skin using PCR, in situ hybridization and double-labeling immunofluorescence. Within the epidermis, the nicotinic (n)ACh-R subunits, α3, α5, β2, and β4 were expressed in the basal cell layer (BCL) and in a single cell layer in the stratum granulosum; α9 was expressed in the basal and lower spinous layers. α7, α10, and β1 were preferentially detected in the upper spinous and granular layers. Of the muscarinic (m)ACh-R, m1 and m4 were found in the suprabasal layers, whereas m2, m3, and m5 remained restricted to the lower layers. In the outer root sheath of the hair follicle, all ACh-R except α9, β1, and m4 were found in the BCL whereas the α9, m4, and m5 ACh-R were restricted to the central cell layer. The α5, β1, β2, m1–m4 chains were strongly expressed in the inner root sheath. Undifferentiated sebocytes expressed the α3, α9, β4, m3–m5 ACh-R whereas α7, β2, β4, m2, and m4 were found in mature sebocytes. In sweat glands, the α3*, α7, and m2–m5 ACh-R were most prominent in the myoepithelial cells whereas α9, β2, m1, m3, and m4 ACh-R were present in the acinar cells. Taken together, our data result in a complete molecular map of the extraneuronal cholinergic system of the skin that may be translated into distinct functional reaction patterns. |
doi_str_mv | 10.1111/j.0022-202X.2004.23425.x |
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Within the epidermis, the nicotinic (n)ACh-R subunits, α3, α5, β2, and β4 were expressed in the basal cell layer (BCL) and in a single cell layer in the stratum granulosum; α9 was expressed in the basal and lower spinous layers. α7, α10, and β1 were preferentially detected in the upper spinous and granular layers. Of the muscarinic (m)ACh-R, m1 and m4 were found in the suprabasal layers, whereas m2, m3, and m5 remained restricted to the lower layers. In the outer root sheath of the hair follicle, all ACh-R except α9, β1, and m4 were found in the BCL whereas the α9, m4, and m5 ACh-R were restricted to the central cell layer. The α5, β1, β2, m1–m4 chains were strongly expressed in the inner root sheath. Undifferentiated sebocytes expressed the α3, α9, β4, m3–m5 ACh-R whereas α7, β2, β4, m2, and m4 were found in mature sebocytes. In sweat glands, the α3*, α7, and m2–m5 ACh-R were most prominent in the myoepithelial cells whereas α9, β2, m1, m3, and m4 ACh-R were present in the acinar cells. Taken together, our data result in a complete molecular map of the extraneuronal cholinergic system of the skin that may be translated into distinct functional reaction patterns.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/j.0022-202X.2004.23425.x</identifier><identifier>PMID: 15482483</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>acetylcholine ; Animals ; Antibodies ; Biological and medical sciences ; Cholinergic Fibers - physiology ; Dermatology ; Epidermis - innervation ; Epidermis - physiology ; Gene Expression Profiling ; Guinea Pigs ; Hair Follicle - physiology ; Humans ; Immunohistochemistry ; Medical sciences ; muscarine ; nicotine ; Phenotype ; Polymerase Chain Reaction ; receptor ; Receptors, Muscarinic - genetics ; Receptors, Muscarinic - metabolism ; Receptors, Nicotinic - genetics ; Receptors, Nicotinic - metabolism ; RNA, Messenger - analysis ; Scalp - innervation ; Scalp - physiology ; Sebaceous Glands - physiology ; sweat ; Sweat Glands - physiology</subject><ispartof>Journal of investigative dermatology, 2004-11, Vol.123 (5), p.937-949</ispartof><rights>2004 The Society for Investigative Dermatology, Inc</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Nov 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-84e381c2f279521c7f9142d3d7cc1dbff0aafeb748c86c95299da83cd62657f73</citedby><cites>FETCH-LOGICAL-c565t-84e381c2f279521c7f9142d3d7cc1dbff0aafeb748c86c95299da83cd62657f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210369992?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16259630$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15482483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kurzen, Hjalmar</creatorcontrib><creatorcontrib>Berger, Hans</creatorcontrib><creatorcontrib>Jäger, Claudia</creatorcontrib><creatorcontrib>Hartschuh, Wolfgang</creatorcontrib><creatorcontrib>Näher, Helmut</creatorcontrib><creatorcontrib>Gratchev, Alexei</creatorcontrib><creatorcontrib>Goerdt, Sergij</creatorcontrib><creatorcontrib>Deichmann, Martin</creatorcontrib><title>Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>We present molecular and protein profiling of all acetylcholine receptors (ACh-R) in human scalp skin using PCR, in situ hybridization and double-labeling immunofluorescence. Within the epidermis, the nicotinic (n)ACh-R subunits, α3, α5, β2, and β4 were expressed in the basal cell layer (BCL) and in a single cell layer in the stratum granulosum; α9 was expressed in the basal and lower spinous layers. α7, α10, and β1 were preferentially detected in the upper spinous and granular layers. Of the muscarinic (m)ACh-R, m1 and m4 were found in the suprabasal layers, whereas m2, m3, and m5 remained restricted to the lower layers. In the outer root sheath of the hair follicle, all ACh-R except α9, β1, and m4 were found in the BCL whereas the α9, m4, and m5 ACh-R were restricted to the central cell layer. The α5, β1, β2, m1–m4 chains were strongly expressed in the inner root sheath. Undifferentiated sebocytes expressed the α3, α9, β4, m3–m5 ACh-R whereas α7, β2, β4, m2, and m4 were found in mature sebocytes. In sweat glands, the α3*, α7, and m2–m5 ACh-R were most prominent in the myoepithelial cells whereas α9, β2, m1, m3, and m4 ACh-R were present in the acinar cells. Taken together, our data result in a complete molecular map of the extraneuronal cholinergic system of the skin that may be translated into distinct functional reaction patterns.</description><subject>acetylcholine</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Cholinergic Fibers - physiology</subject><subject>Dermatology</subject><subject>Epidermis - innervation</subject><subject>Epidermis - physiology</subject><subject>Gene Expression Profiling</subject><subject>Guinea Pigs</subject><subject>Hair Follicle - physiology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>muscarine</subject><subject>nicotine</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>receptor</subject><subject>Receptors, Muscarinic - genetics</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Receptors, Nicotinic - genetics</subject><subject>Receptors, Nicotinic - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Scalp - innervation</subject><subject>Scalp - physiology</subject><subject>Sebaceous Glands - physiology</subject><subject>sweat</subject><subject>Sweat Glands - physiology</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0E1v0zAYwHELMbEy-AigCAluCX6PfYRqwKRNTBpIu1mu83h1SePOTlD77XFpt0lc8MWH52fL_iNUEdyQsj6uGowprSmmtw3FmDeUcSqa7TM0I4KymrS8fY5mj-gUvcx5hTGRXKgX6JQIrihXbIZur5cwxHG3Cc72lR266ir24Kbepuo6RR_6MNxV0VfjEqrz7ZjsAFOKQ8HzZSxDSHfBVTe7PML6wd38CsMrdOJtn-H1cT9DP7-c_5h_qy-_f72Yf7qsnZBirBUHpoijnrZaUOJarwmnHeta50i38B5b62HRcuWUdIVo3VnFXCepFK1v2Rn6cLh3k-L9BHk065Ad9H15aJyykVJLhRUv8N0_cBWnVD6SDSWYSa01LUgdkEsx5wTebFJY27QzBJt9erMy-6pmX9Xs05u_6c22HH17vH9arKF7OnhsXcD7I7C5xPYlpQv5yUkqtGS4uDcHN9hxSvAIhMRUcF3mnw9zKFl_B0gmuwCDgy4kcKPpYvj_a_8AjyOsBg</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Kurzen, Hjalmar</creator><creator>Berger, Hans</creator><creator>Jäger, Claudia</creator><creator>Hartschuh, Wolfgang</creator><creator>Näher, Helmut</creator><creator>Gratchev, Alexei</creator><creator>Goerdt, Sergij</creator><creator>Deichmann, Martin</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin</title><author>Kurzen, Hjalmar ; Berger, Hans ; Jäger, Claudia ; Hartschuh, Wolfgang ; Näher, Helmut ; Gratchev, Alexei ; Goerdt, Sergij ; Deichmann, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-84e381c2f279521c7f9142d3d7cc1dbff0aafeb748c86c95299da83cd62657f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>acetylcholine</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Cholinergic Fibers - physiology</topic><topic>Dermatology</topic><topic>Epidermis - innervation</topic><topic>Epidermis - physiology</topic><topic>Gene Expression Profiling</topic><topic>Guinea Pigs</topic><topic>Hair Follicle - physiology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>muscarine</topic><topic>nicotine</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>receptor</topic><topic>Receptors, Muscarinic - genetics</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Receptors, Nicotinic - genetics</topic><topic>Receptors, Nicotinic - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Scalp - innervation</topic><topic>Scalp - physiology</topic><topic>Sebaceous Glands - physiology</topic><topic>sweat</topic><topic>Sweat Glands - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kurzen, Hjalmar</creatorcontrib><creatorcontrib>Berger, Hans</creatorcontrib><creatorcontrib>Jäger, Claudia</creatorcontrib><creatorcontrib>Hartschuh, Wolfgang</creatorcontrib><creatorcontrib>Näher, Helmut</creatorcontrib><creatorcontrib>Gratchev, Alexei</creatorcontrib><creatorcontrib>Goerdt, Sergij</creatorcontrib><creatorcontrib>Deichmann, Martin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kurzen, Hjalmar</au><au>Berger, Hans</au><au>Jäger, Claudia</au><au>Hartschuh, Wolfgang</au><au>Näher, Helmut</au><au>Gratchev, Alexei</au><au>Goerdt, Sergij</au><au>Deichmann, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>123</volume><issue>5</issue><spage>937</spage><epage>949</epage><pages>937-949</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>We present molecular and protein profiling of all acetylcholine receptors (ACh-R) in human scalp skin using PCR, in situ hybridization and double-labeling immunofluorescence. Within the epidermis, the nicotinic (n)ACh-R subunits, α3, α5, β2, and β4 were expressed in the basal cell layer (BCL) and in a single cell layer in the stratum granulosum; α9 was expressed in the basal and lower spinous layers. α7, α10, and β1 were preferentially detected in the upper spinous and granular layers. Of the muscarinic (m)ACh-R, m1 and m4 were found in the suprabasal layers, whereas m2, m3, and m5 remained restricted to the lower layers. In the outer root sheath of the hair follicle, all ACh-R except α9, β1, and m4 were found in the BCL whereas the α9, m4, and m5 ACh-R were restricted to the central cell layer. The α5, β1, β2, m1–m4 chains were strongly expressed in the inner root sheath. Undifferentiated sebocytes expressed the α3, α9, β4, m3–m5 ACh-R whereas α7, β2, β4, m2, and m4 were found in mature sebocytes. In sweat glands, the α3*, α7, and m2–m5 ACh-R were most prominent in the myoepithelial cells whereas α9, β2, m1, m3, and m4 ACh-R were present in the acinar cells. Taken together, our data result in a complete molecular map of the extraneuronal cholinergic system of the skin that may be translated into distinct functional reaction patterns.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>15482483</pmid><doi>10.1111/j.0022-202X.2004.23425.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | acetylcholine Animals Antibodies Biological and medical sciences Cholinergic Fibers - physiology Dermatology Epidermis - innervation Epidermis - physiology Gene Expression Profiling Guinea Pigs Hair Follicle - physiology Humans Immunohistochemistry Medical sciences muscarine nicotine Phenotype Polymerase Chain Reaction receptor Receptors, Muscarinic - genetics Receptors, Muscarinic - metabolism Receptors, Nicotinic - genetics Receptors, Nicotinic - metabolism RNA, Messenger - analysis Scalp - innervation Scalp - physiology Sebaceous Glands - physiology sweat Sweat Glands - physiology |
title | Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin |
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