Investigations on epiphytic living Pseudomonas species from Malus domestica with an antagonistic effect to Venturia inaequalis on isolated plant cuticle membranes
Summary In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase g...
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| Veröffentlicht in: | Environmental microbiology 2004-11, Vol.6 (11), p.1149-1158 |
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| container_title | Environmental microbiology |
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| creator | Singh, Prikhshayat Piotrowski, Markus Kloppstech, Klaus Gau, Achim E. |
| description | Summary
In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase genes from Vibrio harveyi in order to follow up their development and activity on the isolated cuticles. Our finding was that the epiphytic bacteria can have access to nutrients below the cuticle without causing damage to these cuticular membranes. Bacterial proteins may enable this nutrient mobilization and we found, indeed, that more than 46 proteins that must have been delivered by the bacteria in response to interaction with the cuticles as they could be found below the cuticle membrane. Eight major representatives of this group of external proteins have been sequenced with electron spray quadrupole time of flight mass spectrometry and subsequently identified by data base homology search as a flagellin, a porin type protein and proteins that are involved in amino acid recruitment and metabolism. |
| doi_str_mv | 10.1111/j.1462-2920.2004.00622.x |
| format | Article |
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In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase genes from Vibrio harveyi in order to follow up their development and activity on the isolated cuticles. Our finding was that the epiphytic bacteria can have access to nutrients below the cuticle without causing damage to these cuticular membranes. Bacterial proteins may enable this nutrient mobilization and we found, indeed, that more than 46 proteins that must have been delivered by the bacteria in response to interaction with the cuticles as they could be found below the cuticle membrane. Eight major representatives of this group of external proteins have been sequenced with electron spray quadrupole time of flight mass spectrometry and subsequently identified by data base homology search as a flagellin, a porin type protein and proteins that are involved in amino acid recruitment and metabolism.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/j.1462-2920.2004.00622.x</identifier><identifier>PMID: 15479248</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Antibiosis ; Ascomycota - isolation & purification ; Ascomycota - physiology ; Bacterial Proteins - chemistry ; Flagellin - isolation & purification ; Genes, Reporter ; Luciferases - genetics ; Luciferases - metabolism ; Malus - microbiology ; Malus domestica ; Porins - isolation & purification ; Pseudomonas ; Pseudomonas - isolation & purification ; Pseudomonas - physiology ; Staining and Labeling ; Venturia inaequalis ; Vibrio - genetics ; Vibrio harveyi</subject><ispartof>Environmental microbiology, 2004-11, Vol.6 (11), p.1149-1158</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4332-c1363b5d908b2e629323628521e7af7f116dc56646e3c780bf80859501a8c4f33</citedby><cites>FETCH-LOGICAL-c4332-c1363b5d908b2e629323628521e7af7f116dc56646e3c780bf80859501a8c4f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1462-2920.2004.00622.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1462-2920.2004.00622.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15479248$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Prikhshayat</creatorcontrib><creatorcontrib>Piotrowski, Markus</creatorcontrib><creatorcontrib>Kloppstech, Klaus</creatorcontrib><creatorcontrib>Gau, Achim E.</creatorcontrib><title>Investigations on epiphytic living Pseudomonas species from Malus domestica with an antagonistic effect to Venturia inaequalis on isolated plant cuticle membranes</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase genes from Vibrio harveyi in order to follow up their development and activity on the isolated cuticles. Our finding was that the epiphytic bacteria can have access to nutrients below the cuticle without causing damage to these cuticular membranes. Bacterial proteins may enable this nutrient mobilization and we found, indeed, that more than 46 proteins that must have been delivered by the bacteria in response to interaction with the cuticles as they could be found below the cuticle membrane. Eight major representatives of this group of external proteins have been sequenced with electron spray quadrupole time of flight mass spectrometry and subsequently identified by data base homology search as a flagellin, a porin type protein and proteins that are involved in amino acid recruitment and metabolism.</description><subject>Antibiosis</subject><subject>Ascomycota - isolation & purification</subject><subject>Ascomycota - physiology</subject><subject>Bacterial Proteins - chemistry</subject><subject>Flagellin - isolation & purification</subject><subject>Genes, Reporter</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Malus - microbiology</subject><subject>Malus domestica</subject><subject>Porins - isolation & purification</subject><subject>Pseudomonas</subject><subject>Pseudomonas - isolation & purification</subject><subject>Pseudomonas - physiology</subject><subject>Staining and Labeling</subject><subject>Venturia inaequalis</subject><subject>Vibrio - genetics</subject><subject>Vibrio harveyi</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEoqXlLyCfuCX4I7ETiQtUpV1ooYd-SFwsx5lsvSR2aift7t_hl-J0V8sRLEseed5nZqzXSYIIzkhcH1YZyTlNaUVxRjHOM4w5pdn6RXK4T7zcx4QeJG9CWGFMBBP4dXJAilxUNC8Pk98L-whhNEs1GmcDchbBYIb7zWg06syjsUt0FWBqXO-sCigMoA0E1HrXo0vVTQHF1FxBK_RkxnukbNyjWjpr5lsEbQt6RKNDt2DHyRuFjFXwMKnOPPczwXVqhAYNXQSRniLVAeqhr72yEI6TV63qArzdnUfJzZfT65Pz9OLH2eLk00Wqc8ZoqgnjrC6aCpc1BU4rRhmnZUEJCNWKlhDe6ILznAPTosR1W-KyqApMVKnzlrGj5P227uDdwxSfJHsTNHRxKnBTkJxXXFQC_1NIREmowEUUlluh9i4ED60cvOmV30iC5WykXMnZIzn7JWcj5bORch3Rd7seU91D8xfcORcFH7eCJ9PB5r8Ly9PLRQwinm7xaBKs97jyvySPn6SQd9_PJLu6_fY5_3ouf7I_rZu-WA</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Singh, Prikhshayat</creator><creator>Piotrowski, Markus</creator><creator>Kloppstech, Klaus</creator><creator>Gau, Achim E.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200411</creationdate><title>Investigations on epiphytic living Pseudomonas species from Malus domestica with an antagonistic effect to Venturia inaequalis on isolated plant cuticle membranes</title><author>Singh, Prikhshayat ; Piotrowski, Markus ; Kloppstech, Klaus ; Gau, Achim E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4332-c1363b5d908b2e629323628521e7af7f116dc56646e3c780bf80859501a8c4f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antibiosis</topic><topic>Ascomycota - isolation & purification</topic><topic>Ascomycota - physiology</topic><topic>Bacterial Proteins - chemistry</topic><topic>Flagellin - isolation & purification</topic><topic>Genes, Reporter</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Malus - microbiology</topic><topic>Malus domestica</topic><topic>Porins - isolation & purification</topic><topic>Pseudomonas</topic><topic>Pseudomonas - isolation & purification</topic><topic>Pseudomonas - physiology</topic><topic>Staining and Labeling</topic><topic>Venturia inaequalis</topic><topic>Vibrio - genetics</topic><topic>Vibrio harveyi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Prikhshayat</creatorcontrib><creatorcontrib>Piotrowski, Markus</creatorcontrib><creatorcontrib>Kloppstech, Klaus</creatorcontrib><creatorcontrib>Gau, Achim E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Prikhshayat</au><au>Piotrowski, Markus</au><au>Kloppstech, Klaus</au><au>Gau, Achim E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigations on epiphytic living Pseudomonas species from Malus domestica with an antagonistic effect to Venturia inaequalis on isolated plant cuticle membranes</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2004-11</date><risdate>2004</risdate><volume>6</volume><issue>11</issue><spage>1149</spage><epage>1158</epage><pages>1149-1158</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
In order to understand better the survival and mutual interaction of epiphytic bacteria and fungi on apple plants, bacteria collected from these plants were cultivated on intact adaxial, stoma free cuticle membranes originally obtained from apple. The bacteria were labelled with luciferase genes from Vibrio harveyi in order to follow up their development and activity on the isolated cuticles. Our finding was that the epiphytic bacteria can have access to nutrients below the cuticle without causing damage to these cuticular membranes. Bacterial proteins may enable this nutrient mobilization and we found, indeed, that more than 46 proteins that must have been delivered by the bacteria in response to interaction with the cuticles as they could be found below the cuticle membrane. Eight major representatives of this group of external proteins have been sequenced with electron spray quadrupole time of flight mass spectrometry and subsequently identified by data base homology search as a flagellin, a porin type protein and proteins that are involved in amino acid recruitment and metabolism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15479248</pmid><doi>10.1111/j.1462-2920.2004.00622.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Environmental microbiology, 2004-11, Vol.6 (11), p.1149-1158 |
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| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Antibiosis Ascomycota - isolation & purification Ascomycota - physiology Bacterial Proteins - chemistry Flagellin - isolation & purification Genes, Reporter Luciferases - genetics Luciferases - metabolism Malus - microbiology Malus domestica Porins - isolation & purification Pseudomonas Pseudomonas - isolation & purification Pseudomonas - physiology Staining and Labeling Venturia inaequalis Vibrio - genetics Vibrio harveyi |
| title | Investigations on epiphytic living Pseudomonas species from Malus domestica with an antagonistic effect to Venturia inaequalis on isolated plant cuticle membranes |
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