Analysis of genes isolated from lipopolysaccharide-stimulated rainbow trout ( Oncorhynchus mykiss) macrophages

A primary cell culture system was used to obtain differentiated rainbow trout ( Oncorhynchus mykiss) macrophages that were stimulated with Escherichia coli lipopolysaccharide (LPS-10 μg/ml) for 12 h in vitro. Messenger RNA from the LPS-stimulated cells was used to create two cDNA libraries from whic...

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Veröffentlicht in:	Molecular immunology 2004-11, Vol.41 (12), p.1199-1210
Hauptverfasser:	Goetz, Frederick W., Iliev, Dimitar B., McCauley, Linda A.R., Liarte, Cristina Q., Tort, Lluis B., Planas, Josep V., MacKenzie, Simon
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals cDNA libraries Cells, Cultured Escherichia coli Gene Expression Regulation - drug effects Gene Expression Regulation - immunology Gene Library Genes Head kidney Immunity - genetics Lipopolysaccharide Lipopolysaccharides - pharmacology Macrophage Macrophages - immunology Macrophages - metabolism Oncorhynchus mykiss Rainbow trout RNA, Messenger - analysis Transcriptomics Up-Regulation - drug effects Up-Regulation - immunology
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