Rational design of lipid for membrane protein crystallization
The lipidic cubic phase has been used to grow crystals of membrane proteins for high-resolution structure determination. However, the original, so-called, in meso method does not work reliably at low temperatures, where proteins are generally more stable, because the hosting lipid turns solid. The n...
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| Veröffentlicht in: | Journal of structural biology 2004-11, Vol.148 (2), p.169-175 |
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| container_title | Journal of structural biology |
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| creator | Misquitta, Yohann Cherezov, Vadim Havas, Fabien Patterson, Suzanne Mohan, Jakkam M. Wells, Angela J. Hart, David J. Caffrey, Martin |
| description | The lipidic cubic phase has been used to grow crystals of membrane proteins for high-resolution structure determination. However, the original, so-called, in meso method does not work reliably at low temperatures, where proteins are generally more stable, because the hosting lipid turns solid. The need existed therefore for a lipid that forms the cubic phase and that supports crystal growth at low temperatures. We created a database of phase diagrams and used it to design such a lipid. X-ray diffraction showed that the new lipid exhibits designed phase behavior. Further, it produces diffraction quality membrane protein crystals by the in meso method at 6
°C. This demonstrates that lipidic materials, like their protein counterparts are amenable to rational design. The same approach as used in this study should find application in extending the range of membrane proteins crystallizable by the in meso method and in tailoring transport of cubic phases for controlled delivery and uptake. |
| doi_str_mv | 10.1016/j.jsb.2004.06.008 |
| format | Article |
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°C. This demonstrates that lipidic materials, like their protein counterparts are amenable to rational design. The same approach as used in this study should find application in extending the range of membrane proteins crystallizable by the in meso method and in tailoring transport of cubic phases for controlled delivery and uptake.</description><subject>Carbon - chemistry</subject><subject>Crystallization - methods</subject><subject>Crystallography, X-Ray - methods</subject><subject>Cubic phase</subject><subject>Glycerol - chemistry</subject><subject>Halobacterium salinarum - metabolism</subject><subject>Lipids - chemistry</subject><subject>Membrane Proteins - chemistry</subject><subject>Monoacylglycerol</subject><subject>Phase diagram</subject><subject>Protein Conformation</subject><subject>Structure</subject><subject>Temperature</subject><subject>Water - chemistry</subject><subject>X-Ray Diffraction</subject><issn>1047-8477</issn><issn>1095-8657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMorq7-AC_Sk7fWTNskLeJBFr9gQRA9hzSZSEo_1qQrrL_erLvgzdOE4XlfJg8hF0AzoMCv26wNTZZTWmaUZ5RWB-QEaM3SijNxuH2XIq1KIWbkNISWRhByOCYzYHFJa3FCbl_V5MZBdYnB4D6GZLRJ51bOJHb0SY9949WAycqPE7oh0X4TJtV17vs3dkaOrOoCnu_nnLw_3L8tntLly-Pz4m6Z6hLYlBpVQdEANLYshDA8R5rnAlChtlDllTCqqGyhGDeVVTFTsoZypsFCo5mgxZxc7XrjHZ9rDJPsXdDYdfG2cR0k5zVntWARhB2o_RiCRytX3vXKbyRQuXUmWxmdya0zSbmMzmLmcl--bno0f4m9pAjc7ACMX_xy6GXQDgeNxnnUkzSj-6f-B22wfKY</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Misquitta, Yohann</creator><creator>Cherezov, Vadim</creator><creator>Havas, Fabien</creator><creator>Patterson, Suzanne</creator><creator>Mohan, Jakkam M.</creator><creator>Wells, Angela J.</creator><creator>Hart, David J.</creator><creator>Caffrey, Martin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Rational design of lipid for membrane protein crystallization</title><author>Misquitta, Yohann ; Cherezov, Vadim ; Havas, Fabien ; Patterson, Suzanne ; Mohan, Jakkam M. ; Wells, Angela J. ; Hart, David J. ; Caffrey, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-da813b11bf4377d62e02271eaecf18287da38f3a56d8fac4145b065c1f1bc5703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Carbon - chemistry</topic><topic>Crystallization - methods</topic><topic>Crystallography, X-Ray - methods</topic><topic>Cubic phase</topic><topic>Glycerol - chemistry</topic><topic>Halobacterium salinarum - metabolism</topic><topic>Lipids - chemistry</topic><topic>Membrane Proteins - chemistry</topic><topic>Monoacylglycerol</topic><topic>Phase diagram</topic><topic>Protein Conformation</topic><topic>Structure</topic><topic>Temperature</topic><topic>Water - chemistry</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Misquitta, Yohann</creatorcontrib><creatorcontrib>Cherezov, Vadim</creatorcontrib><creatorcontrib>Havas, Fabien</creatorcontrib><creatorcontrib>Patterson, Suzanne</creatorcontrib><creatorcontrib>Mohan, Jakkam M.</creatorcontrib><creatorcontrib>Wells, Angela J.</creatorcontrib><creatorcontrib>Hart, David J.</creatorcontrib><creatorcontrib>Caffrey, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of structural biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Misquitta, Yohann</au><au>Cherezov, Vadim</au><au>Havas, Fabien</au><au>Patterson, Suzanne</au><au>Mohan, Jakkam M.</au><au>Wells, Angela J.</au><au>Hart, David J.</au><au>Caffrey, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rational design of lipid for membrane protein crystallization</atitle><jtitle>Journal of structural biology</jtitle><addtitle>J Struct Biol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>148</volume><issue>2</issue><spage>169</spage><epage>175</epage><pages>169-175</pages><issn>1047-8477</issn><eissn>1095-8657</eissn><abstract>The lipidic cubic phase has been used to grow crystals of membrane proteins for high-resolution structure determination. 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°C. This demonstrates that lipidic materials, like their protein counterparts are amenable to rational design. The same approach as used in this study should find application in extending the range of membrane proteins crystallizable by the in meso method and in tailoring transport of cubic phases for controlled delivery and uptake.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15477097</pmid><doi>10.1016/j.jsb.2004.06.008</doi><tpages>7</tpages></addata></record> |
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| subjects | Carbon - chemistry Crystallization - methods Crystallography, X-Ray - methods Cubic phase Glycerol - chemistry Halobacterium salinarum - metabolism Lipids - chemistry Membrane Proteins - chemistry Monoacylglycerol Phase diagram Protein Conformation Structure Temperature Water - chemistry X-Ray Diffraction |
| title | Rational design of lipid for membrane protein crystallization |
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