Purification and biochemical characterization of a native invertase from the hydrogen-producing Thermotoga neapolitana (DSM 4359)

This is the first report describing the purification and enzymatic properties of a native invertase (β-D-fructosidase) in Thermotogales. The invertase of the hydrogen-producing thermophilic bacterium Thermotoga neapolitana DSM 4359 (hereby named Tni) was a monomer of about 47 kDa having an amino aci...

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Veröffentlicht in:	Extremophiles : life under extreme conditions 2009-03, Vol.13 (2), p.345-354
Hauptverfasser:	Dipasquale, Laura, Gambacorta, Agata, Siciliano, Rosa Anna, Mazzeo, Maria Fiorella, Lama, Licia
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacteria Bacteriology beta-Fructofuranosidase - chemistry Biochemistry Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Enzymes Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydrolysis Inulin - chemistry Ions Kinetics Metabolism. Enzymes Metals - chemistry Microbiology Mission oriented research Models, Chemical Physiology and metabolism Polymers Polymers - chemistry Proteins Space life sciences Substrate Specificity Temperature Thermotoga neapolitana Thermotoga neapolitana - enzymology Thermotogales
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