Expression and purification of human TRPV1 in baculovirus-infected insect cells for structural studies

TRPV1 is a ligand-gated cation channel that is involved in acute thermal nociception and neurogenic inflammation. By using the GP67 signal peptide, high levels of full-length human TRPV1 was expressed in High Five insect cells using the baculovirus expression system. The functional activity of the e...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein expression and purification 2009-05, Vol.65 (1), p.38-50
Hauptverfasser:	Korepanova, Alla, Pereda-Lopez, Ana, Solomon, Larry R., Walter, Karl A., Lake, Marc R., Bianchi, Bruce R., McDonald, Heath A., Neelands, Torben R., Shen, Jianwei, Matayoshi, Edmund D., Moreland, Robert B., Chiu, Mark L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Baculoviridae Cell Line Expression Gene Expression Humans Ligand binding Purification Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Spodoptera TRPV Cation Channels - biosynthesis TRPV Cation Channels - chemistry TRPV Cation Channels - genetics TRPV Cation Channels - isolation & purification TRPV1 Vanilloid receptor
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!