Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2
Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide Δ 4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihyd...
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| Veröffentlicht in: | FEBS letters 2004-10, Vol.576 (1), p.63-67 |
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| creator | Omae, Fumio Miyazaki, Masao Enomoto, Ayako Suzuki, Akemi |
| description | Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide Δ
4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide Δ
4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and
Xenopus. |
| doi_str_mv | 10.1016/j.febslet.2004.08.060 |
| format | Article |
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4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide Δ
4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and
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4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide Δ
4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and
Xenopus.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>C-4 hydroxylase</subject><subject>Chlorocebus aethiops</subject><subject>Conserved Sequence</subject><subject>COS Cells</subject><subject>DES1</subject><subject>DES2</subject><subject>Hydrolases - chemistry</subject><subject>Hydrolases - genetics</subject><subject>Hydrolases - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - chemistry</subject><subject>Multienzyme Complexes - genetics</subject><subject>Oxidoreductases - chemistry</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Δ 4 desaturase</subject><subject>Δ4 desaturase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUlvFDEQhS0EIpPlJ4B84tZNVS9eTggmExIpEofA2XK7q4VHvQS7J9D_Pu7MSBzhZNXTq-enrxh7h5AjoPi4zztqYk9zXgBUOagcBLxiG1SyzMpKqNdsA4BVVktdnrHzGPeQZoX6LTvDupIVIG5Yc9fSOPvOOzv7aeRTx-3IKcZVtT2P9OtAoyPeTYG3_ufShslRsINviW-zir8of5beRuLWzf7Jz8uaMkyHpFzvHopL9qazfaSr03vBftzsvm9vs_tvX--2n-8zV5daZUprKDpCh9gogbUoS6FqJRptncWmLqHRsgAltbayECWiUxqpICicQ1mXF-zDMfcxTKl0nM3go6O-tyOlMkYILaCSMhnro9GFKcZAnXkMfrBhMQhmhWv25gTXrHANKJPgpr33pw8OzUDt360TzWS4PRp--56W_0s1N7svxcN6qfVQkHJAapWiPh2jKBF78hRMdH49ROsDudm0k_9H22dZJ6IH</recordid><startdate>20041008</startdate><enddate>20041008</enddate><creator>Omae, Fumio</creator><creator>Miyazaki, Masao</creator><creator>Enomoto, Ayako</creator><creator>Suzuki, Akemi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041008</creationdate><title>Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2</title><author>Omae, Fumio ; Miyazaki, Masao ; Enomoto, Ayako ; Suzuki, Akemi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5398-89902fe1c11b861563368586b9aca1b530b97208799a726311c891e2e02cc1753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>C-4 hydroxylase</topic><topic>Chlorocebus aethiops</topic><topic>Conserved Sequence</topic><topic>COS Cells</topic><topic>DES1</topic><topic>DES2</topic><topic>Hydrolases - chemistry</topic><topic>Hydrolases - genetics</topic><topic>Hydrolases - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - chemistry</topic><topic>Multienzyme Complexes - genetics</topic><topic>Oxidoreductases - chemistry</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Δ 4 desaturase</topic><topic>Δ4 desaturase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Omae, Fumio</creatorcontrib><creatorcontrib>Miyazaki, Masao</creatorcontrib><creatorcontrib>Enomoto, Ayako</creatorcontrib><creatorcontrib>Suzuki, Akemi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Omae, Fumio</au><au>Miyazaki, Masao</au><au>Enomoto, Ayako</au><au>Suzuki, Akemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-10-08</date><risdate>2004</risdate><volume>576</volume><issue>1</issue><spage>63</spage><epage>67</epage><pages>63-67</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide Δ
4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide Δ
4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and
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| subjects | Amino Acid Sequence Animals C-4 hydroxylase Chlorocebus aethiops Conserved Sequence COS Cells DES1 DES2 Hydrolases - chemistry Hydrolases - genetics Hydrolases - metabolism Mice Molecular Sequence Data Multienzyme Complexes - chemistry Multienzyme Complexes - genetics Oxidoreductases - chemistry Oxidoreductases - genetics Oxidoreductases - metabolism Phylogeny Recombinant Proteins - chemistry Recombinant Proteins - metabolism Sequence Homology, Amino Acid Δ 4 desaturase Δ4 desaturase |
| title | Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2 |
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