Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP
The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation ti...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2004-10, Vol.576 (1), p.216-220 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 220 |
|---|---|
| container_issue | 1 |
| container_start_page | 216 |
| container_title | FEBS letters |
| container_volume | 576 |
| creator | Leboulle, Gérard Müller, Uli |
| description | The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presence of very low cAMP concentrations, we found a synergistic increase in PKAII activation by physiological cGMP concentrations. Cloning honeybee regulatory subunit RII and phylogenetic comparison of the two cyclic nucleotide-binding sites of RII reveal a high relation of domain A of insect RII with cGMP-binding domains of cGMP-dependent protein kinases. |
| doi_str_mv | 10.1016/j.febslet.2004.08.079 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66959793</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579304011160</els_id><sourcerecordid>66959793</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5700-f56a493369968952aa30efed643f63faf5cf0decf1743a04bf44851379ecc1353</originalsourceid><addsrcrecordid>eNqNkc1r3DAQxUVpabZp_oQWnUp6sDtafdg6lU3Ix0JCA2nOQiuPirZeeWt5E_zfV2YNPaanYeC9N8P7EfKJQcmAqW_b0uMmtTiUSwBRQl1Cpd-QBasrXnCh6rdkAcBEISvNT8iHlLaQ95rp9-SESVEJELAg-DhG7H-FNARHrRvCsx1CF2nnaYgJ3UDd6v6haHCPscE40H3fDRgi_R2iTUhX9HwY90jX6690M1I3ujZMDmpjM2839w8fyTtv24Rn8zwlT9dXPy9vi7sfN-vL1V3hZAVQeKms0JwrrVWt5dJaDuixUYJ7xb310nlo0HlWCW5BbLwQtWS80ugc45Kfki_H3PzlnwOmwexCcti2NmJ3SEYpLXXu41XhkoGUmldZKI9C13cp9ejNvg8724-GgZlAmK2ZQZgJhIHaZBDZ93k-cNjssPnnmpvPgtuj4CW0OP5fqrm-ulg-TlQnqDmFMaamqO_HKMzVPgfsTXIBo8Mm9Jmgabrwyrd_AWTHsMc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21055937</pqid></control><display><type>article</type><title>Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP</title><source>MEDLINE</source><source>Wiley Free Content</source><source>ScienceDirect Journals (5 years ago - present)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Leboulle, Gérard ; Müller, Uli</creator><creatorcontrib>Leboulle, Gérard ; Müller, Uli</creatorcontrib><description>The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presence of very low cAMP concentrations, we found a synergistic increase in PKAII activation by physiological cGMP concentrations. Cloning honeybee regulatory subunit RII and phylogenetic comparison of the two cyclic nucleotide-binding sites of RII reveal a high relation of domain A of insect RII with cGMP-binding domains of cGMP-dependent protein kinases.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2004.08.079</identifier><identifier>PMID: 15474040</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Apis mellifera ; Bees - metabolism ; Binding Sites ; C, catalytic subunit ; Computational Biology ; Cyclic AMP ; Cyclic AMP - metabolism ; Cyclic AMP-Dependent Protein Kinase Type II ; Cyclic AMP-Dependent Protein Kinases - isolation & purification ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Cyclic cGMP ; Cyclic GMP - metabolism ; Enzyme Activation ; Insect ; Nucleotide-binding site ; PBC, phosphate-binding cassette ; Phylogeny ; PKA, cAMP-dependent protein kinase ; PKG, cGMP-dependent protein kinase ; Protein kinase A ; R, regulatory subunit ; Regulatory subunit</subject><ispartof>FEBS letters, 2004-10, Vol.576 (1), p.216-220</ispartof><rights>2004</rights><rights>FEBS Letters 576 (2004) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright 2004 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5700-f56a493369968952aa30efed643f63faf5cf0decf1743a04bf44851379ecc1353</citedby><cites>FETCH-LOGICAL-c5700-f56a493369968952aa30efed643f63faf5cf0decf1743a04bf44851379ecc1353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2004.08.079$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2004.08.079$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3549,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15474040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leboulle, Gérard</creatorcontrib><creatorcontrib>Müller, Uli</creatorcontrib><title>Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presence of very low cAMP concentrations, we found a synergistic increase in PKAII activation by physiological cGMP concentrations. Cloning honeybee regulatory subunit RII and phylogenetic comparison of the two cyclic nucleotide-binding sites of RII reveal a high relation of domain A of insect RII with cGMP-binding domains of cGMP-dependent protein kinases.</description><subject>Animals</subject><subject>Apis mellifera</subject><subject>Bees - metabolism</subject><subject>Binding Sites</subject><subject>C, catalytic subunit</subject><subject>Computational Biology</subject><subject>Cyclic AMP</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic AMP-Dependent Protein Kinase Type II</subject><subject>Cyclic AMP-Dependent Protein Kinases - isolation & purification</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Cyclic cGMP</subject><subject>Cyclic GMP - metabolism</subject><subject>Enzyme Activation</subject><subject>Insect</subject><subject>Nucleotide-binding site</subject><subject>PBC, phosphate-binding cassette</subject><subject>Phylogeny</subject><subject>PKA, cAMP-dependent protein kinase</subject><subject>PKG, cGMP-dependent protein kinase</subject><subject>Protein kinase A</subject><subject>R, regulatory subunit</subject><subject>Regulatory subunit</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1r3DAQxUVpabZp_oQWnUp6sDtafdg6lU3Ix0JCA2nOQiuPirZeeWt5E_zfV2YNPaanYeC9N8P7EfKJQcmAqW_b0uMmtTiUSwBRQl1Cpd-QBasrXnCh6rdkAcBEISvNT8iHlLaQ95rp9-SESVEJELAg-DhG7H-FNARHrRvCsx1CF2nnaYgJ3UDd6v6haHCPscE40H3fDRgi_R2iTUhX9HwY90jX6690M1I3ujZMDmpjM2839w8fyTtv24Rn8zwlT9dXPy9vi7sfN-vL1V3hZAVQeKms0JwrrVWt5dJaDuixUYJ7xb310nlo0HlWCW5BbLwQtWS80ugc45Kfki_H3PzlnwOmwexCcti2NmJ3SEYpLXXu41XhkoGUmldZKI9C13cp9ejNvg8724-GgZlAmK2ZQZgJhIHaZBDZ93k-cNjssPnnmpvPgtuj4CW0OP5fqrm-ulg-TlQnqDmFMaamqO_HKMzVPgfsTXIBo8Mm9Jmgabrwyrd_AWTHsMc</recordid><startdate>20041008</startdate><enddate>20041008</enddate><creator>Leboulle, Gérard</creator><creator>Müller, Uli</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20041008</creationdate><title>Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP</title><author>Leboulle, Gérard ; Müller, Uli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5700-f56a493369968952aa30efed643f63faf5cf0decf1743a04bf44851379ecc1353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Apis mellifera</topic><topic>Bees - metabolism</topic><topic>Binding Sites</topic><topic>C, catalytic subunit</topic><topic>Computational Biology</topic><topic>Cyclic AMP</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic AMP-Dependent Protein Kinase Type II</topic><topic>Cyclic AMP-Dependent Protein Kinases - isolation & purification</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Cyclic cGMP</topic><topic>Cyclic GMP - metabolism</topic><topic>Enzyme Activation</topic><topic>Insect</topic><topic>Nucleotide-binding site</topic><topic>PBC, phosphate-binding cassette</topic><topic>Phylogeny</topic><topic>PKA, cAMP-dependent protein kinase</topic><topic>PKG, cGMP-dependent protein kinase</topic><topic>Protein kinase A</topic><topic>R, regulatory subunit</topic><topic>Regulatory subunit</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leboulle, Gérard</creatorcontrib><creatorcontrib>Müller, Uli</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leboulle, Gérard</au><au>Müller, Uli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-10-08</date><risdate>2004</risdate><volume>576</volume><issue>1</issue><spage>216</spage><epage>220</epage><pages>216-220</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presence of very low cAMP concentrations, we found a synergistic increase in PKAII activation by physiological cGMP concentrations. Cloning honeybee regulatory subunit RII and phylogenetic comparison of the two cyclic nucleotide-binding sites of RII reveal a high relation of domain A of insect RII with cGMP-binding domains of cGMP-dependent protein kinases.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>15474040</pmid><doi>10.1016/j.febslet.2004.08.079</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2004-10, Vol.576 (1), p.216-220 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66959793 |
| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Apis mellifera Bees - metabolism Binding Sites C, catalytic subunit Computational Biology Cyclic AMP Cyclic AMP - metabolism Cyclic AMP-Dependent Protein Kinase Type II Cyclic AMP-Dependent Protein Kinases - isolation & purification Cyclic AMP-Dependent Protein Kinases - metabolism Cyclic cGMP Cyclic GMP - metabolism Enzyme Activation Insect Nucleotide-binding site PBC, phosphate-binding cassette Phylogeny PKA, cAMP-dependent protein kinase PKG, cGMP-dependent protein kinase Protein kinase A R, regulatory subunit Regulatory subunit |
| title | Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T15%3A30%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synergistic%20activation%20of%20insect%20cAMP-dependent%20protein%20kinase%20A%20(type%20II)%20by%20cyclicAMP%20and%20cyclicGMP&rft.jtitle=FEBS%20letters&rft.au=Leboulle,%20G%C3%A9rard&rft.date=2004-10-08&rft.volume=576&rft.issue=1&rft.spage=216&rft.epage=220&rft.pages=216-220&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/j.febslet.2004.08.079&rft_dat=%3Cproquest_cross%3E66959793%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21055937&rft_id=info:pmid/15474040&rft_els_id=S0014579304011160&rfr_iscdi=true |