Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription
CYP6B4, a cytochrome P450 gene from the tiger swallowtail Papilio glaucus, is transcriptionally induced in the midgut by dietary furanocoumarins, plant allelochemicals that can crosslink DNA in their UV-activated form. The CYP6B4 promoter contains an overlapping EcRE/ARE/XRE-xan element similar to t...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2004-10, Vol.34 (10), p.1129-1139 |
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| creator | McDonnell, Cynthia M. Brown, Rebecca Petersen Berenbaum, May R. Schuler, Mary A. |
| description | CYP6B4, a cytochrome P450 gene from the tiger swallowtail
Papilio glaucus, is transcriptionally induced in the midgut by dietary furanocoumarins, plant allelochemicals that can crosslink DNA in their UV-activated form. The
CYP6B4 promoter contains an overlapping EcRE/ARE/XRE-xan element similar to that used for basal and xanthotoxin-inducible expression of the
CYP6B1 promoter from the black swallowtail
Papilio polyxenes. Transfection of the
CYP6B4 promoter:CAT reporter construct into Sf9 cells demonstrates that the basal and xanthotoxin-inducible expression levels observed reflect the relative expression levels of this gene in the midguts of tiger swallowtail larvae. Transfections of mutant
CYP6B4 promoter constructs into Sf9 cells indicate that the EcRE/ARE/XRE-xan element is necessary for
CYP6B4 induction by xanthotoxin but not for its minimal basal expression. In addition to these elements, the
CYP6B4 and
CYP6B1 promoters also contain putative XRE-AhR elements identical to the aryl hydrocarbon response elements present in mammalian phase I detoxification genes. Transfections of
CYP6B4 and
CYP6B1 promoters containing EcRE/ARE/XRE-xan and XRE-AhR elements indicate that both are induced significantly by benzo(α)pyrene, an aryl hydrocarbon widespread in the environment, as well as by xanthotoxin, an allelochemical encountered in their hostplants. |
| doi_str_mv | 10.1016/j.ibmb.2004.06.014 |
| format | Article |
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Papilio glaucus, is transcriptionally induced in the midgut by dietary furanocoumarins, plant allelochemicals that can crosslink DNA in their UV-activated form. The
CYP6B4 promoter contains an overlapping EcRE/ARE/XRE-xan element similar to that used for basal and xanthotoxin-inducible expression of the
CYP6B1 promoter from the black swallowtail
Papilio polyxenes. Transfection of the
CYP6B4 promoter:CAT reporter construct into Sf9 cells demonstrates that the basal and xanthotoxin-inducible expression levels observed reflect the relative expression levels of this gene in the midguts of tiger swallowtail larvae. Transfections of mutant
CYP6B4 promoter constructs into Sf9 cells indicate that the EcRE/ARE/XRE-xan element is necessary for
CYP6B4 induction by xanthotoxin but not for its minimal basal expression. In addition to these elements, the
CYP6B4 and
CYP6B1 promoters also contain putative XRE-AhR elements identical to the aryl hydrocarbon response elements present in mammalian phase I detoxification genes. Transfections of
CYP6B4 and
CYP6B1 promoters containing EcRE/ARE/XRE-xan and XRE-AhR elements indicate that both are induced significantly by benzo(α)pyrene, an aryl hydrocarbon widespread in the environment, as well as by xanthotoxin, an allelochemical encountered in their hostplants.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2004.06.014</identifier><identifier>PMID: 15475306</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>allelochemicals ; Animals ; Aryl Hydrocarbon Hydroxylases - genetics ; Aryl hydrocarbon responses ; Base Sequence ; Benzo(a)pyrene - pharmacology ; benzopyrene ; butterflies ; Butterflies - drug effects ; Butterflies - enzymology ; Butterflies - genetics ; Conserved Sequence ; conserved sequences ; coumarins ; CYP6B1 promoter ; CYP6B4 promoter ; cytochrome P-450 ; Cytochrome P450s ; deletion mutants ; DNA - genetics ; Furanocoumarin responses ; Furocoumarins - pharmacology ; gene expression ; gene induction ; Genes, Insect - drug effects ; Insect–plant interactions ; metabolic detoxification ; methoxsalen ; Methoxsalen - pharmacology ; Molecular Sequence Data ; mutants ; Mutation ; Papilio ; Papilio glaucus ; Papilionidae ; promoter regions ; Promoter Regions, Genetic - drug effects ; psoralens ; reporter genes ; response elements ; Sequence Deletion ; Sequence Homology, Nucleic Acid ; transcription (genetics) ; Transcription, Genetic - drug effects ; Transcriptional regulation ; Transfection</subject><ispartof>Insect biochemistry and molecular biology, 2004-10, Vol.34 (10), p.1129-1139</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-c4210b469e54538a50a55bfba26903aadb51882d54156965778b3060a88e6c343</citedby><cites>FETCH-LOGICAL-c407t-c4210b469e54538a50a55bfba26903aadb51882d54156965778b3060a88e6c343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0965174804001213$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15475306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McDonnell, Cynthia M.</creatorcontrib><creatorcontrib>Brown, Rebecca Petersen</creatorcontrib><creatorcontrib>Berenbaum, May R.</creatorcontrib><creatorcontrib>Schuler, Mary A.</creatorcontrib><title>Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>CYP6B4, a cytochrome P450 gene from the tiger swallowtail
Papilio glaucus, is transcriptionally induced in the midgut by dietary furanocoumarins, plant allelochemicals that can crosslink DNA in their UV-activated form. The
CYP6B4 promoter contains an overlapping EcRE/ARE/XRE-xan element similar to that used for basal and xanthotoxin-inducible expression of the
CYP6B1 promoter from the black swallowtail
Papilio polyxenes. Transfection of the
CYP6B4 promoter:CAT reporter construct into Sf9 cells demonstrates that the basal and xanthotoxin-inducible expression levels observed reflect the relative expression levels of this gene in the midguts of tiger swallowtail larvae. Transfections of mutant
CYP6B4 promoter constructs into Sf9 cells indicate that the EcRE/ARE/XRE-xan element is necessary for
CYP6B4 induction by xanthotoxin but not for its minimal basal expression. In addition to these elements, the
CYP6B4 and
CYP6B1 promoters also contain putative XRE-AhR elements identical to the aryl hydrocarbon response elements present in mammalian phase I detoxification genes. Transfections of
CYP6B4 and
CYP6B1 promoters containing EcRE/ARE/XRE-xan and XRE-AhR elements indicate that both are induced significantly by benzo(α)pyrene, an aryl hydrocarbon widespread in the environment, as well as by xanthotoxin, an allelochemical encountered in their hostplants.</description><subject>allelochemicals</subject><subject>Animals</subject><subject>Aryl Hydrocarbon Hydroxylases - genetics</subject><subject>Aryl hydrocarbon responses</subject><subject>Base Sequence</subject><subject>Benzo(a)pyrene - pharmacology</subject><subject>benzopyrene</subject><subject>butterflies</subject><subject>Butterflies - drug effects</subject><subject>Butterflies - enzymology</subject><subject>Butterflies - genetics</subject><subject>Conserved Sequence</subject><subject>conserved sequences</subject><subject>coumarins</subject><subject>CYP6B1 promoter</subject><subject>CYP6B4 promoter</subject><subject>cytochrome P-450</subject><subject>Cytochrome P450s</subject><subject>deletion mutants</subject><subject>DNA - genetics</subject><subject>Furanocoumarin responses</subject><subject>Furocoumarins - pharmacology</subject><subject>gene expression</subject><subject>gene induction</subject><subject>Genes, Insect - drug effects</subject><subject>Insect–plant interactions</subject><subject>metabolic detoxification</subject><subject>methoxsalen</subject><subject>Methoxsalen - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>Mutation</subject><subject>Papilio</subject><subject>Papilio glaucus</subject><subject>Papilionidae</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - drug effects</subject><subject>psoralens</subject><subject>reporter genes</subject><subject>response elements</subject><subject>Sequence Deletion</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcriptional regulation</subject><subject>Transfection</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhS0EYsrAC7AAr9glXCe2k0hsUDX8SCOBBLO2HOem48qxi-0M6ivw1LhqETvY2JL1neN7zyHkJYOaAZNv97Udl7FuAHgNsgbGH5EN67uhgobDY7KBQYqKdby_Is9S2kMBueiekismeCdakBvyaxt8wviAE424W53OIR4pOlzQ50Stp_ke6SGGJWSMiYaZ5p-BaufQBXOPizXaVdZPq7GjQ2qOuTwXHOlXLoDu0GOik51njMXRFuHxz09Ic9Q-mWgP2Qb_nDyZtUv44nJfk7sPN9-3n6rbLx8_b9_fVoZDl8vZMBi5HFBw0fZagBZinEfdyAFaradRsL5vJsGZkCWAruvHsirovkdpWt5ekzdn37LVjxVTVotNBp3THsOalJSDGDj8H2Rdx1nJsYDNGTQxpBRxVodoFx2PioE6VaX26lSVOlWlQKpSVRG9uriv44LTX8mlmwK8PgOzDkrvok3q7lsDrAUYhCwBFOLdmcAS14PFqJKx6A1ONqLJagr2XxP8Bm0EsF0</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>McDonnell, Cynthia M.</creator><creator>Brown, Rebecca Petersen</creator><creator>Berenbaum, May R.</creator><creator>Schuler, Mary A.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription</title><author>McDonnell, Cynthia M. ; Brown, Rebecca Petersen ; Berenbaum, May R. ; Schuler, Mary A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-c4210b469e54538a50a55bfba26903aadb51882d54156965778b3060a88e6c343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>allelochemicals</topic><topic>Animals</topic><topic>Aryl Hydrocarbon Hydroxylases - genetics</topic><topic>Aryl hydrocarbon responses</topic><topic>Base Sequence</topic><topic>Benzo(a)pyrene - pharmacology</topic><topic>benzopyrene</topic><topic>butterflies</topic><topic>Butterflies - drug effects</topic><topic>Butterflies - enzymology</topic><topic>Butterflies - genetics</topic><topic>Conserved Sequence</topic><topic>conserved sequences</topic><topic>coumarins</topic><topic>CYP6B1 promoter</topic><topic>CYP6B4 promoter</topic><topic>cytochrome P-450</topic><topic>Cytochrome P450s</topic><topic>deletion mutants</topic><topic>DNA - genetics</topic><topic>Furanocoumarin responses</topic><topic>Furocoumarins - pharmacology</topic><topic>gene expression</topic><topic>gene induction</topic><topic>Genes, Insect - drug effects</topic><topic>Insect–plant interactions</topic><topic>metabolic detoxification</topic><topic>methoxsalen</topic><topic>Methoxsalen - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>Mutation</topic><topic>Papilio</topic><topic>Papilio glaucus</topic><topic>Papilionidae</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - drug effects</topic><topic>psoralens</topic><topic>reporter genes</topic><topic>response elements</topic><topic>Sequence Deletion</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcriptional regulation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McDonnell, Cynthia M.</creatorcontrib><creatorcontrib>Brown, Rebecca Petersen</creatorcontrib><creatorcontrib>Berenbaum, May R.</creatorcontrib><creatorcontrib>Schuler, Mary A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McDonnell, Cynthia M.</au><au>Brown, Rebecca Petersen</au><au>Berenbaum, May R.</au><au>Schuler, Mary A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>34</volume><issue>10</issue><spage>1129</spage><epage>1139</epage><pages>1129-1139</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>CYP6B4, a cytochrome P450 gene from the tiger swallowtail
Papilio glaucus, is transcriptionally induced in the midgut by dietary furanocoumarins, plant allelochemicals that can crosslink DNA in their UV-activated form. The
CYP6B4 promoter contains an overlapping EcRE/ARE/XRE-xan element similar to that used for basal and xanthotoxin-inducible expression of the
CYP6B1 promoter from the black swallowtail
Papilio polyxenes. Transfection of the
CYP6B4 promoter:CAT reporter construct into Sf9 cells demonstrates that the basal and xanthotoxin-inducible expression levels observed reflect the relative expression levels of this gene in the midguts of tiger swallowtail larvae. Transfections of mutant
CYP6B4 promoter constructs into Sf9 cells indicate that the EcRE/ARE/XRE-xan element is necessary for
CYP6B4 induction by xanthotoxin but not for its minimal basal expression. In addition to these elements, the
CYP6B4 and
CYP6B1 promoters also contain putative XRE-AhR elements identical to the aryl hydrocarbon response elements present in mammalian phase I detoxification genes. Transfections of
CYP6B4 and
CYP6B1 promoters containing EcRE/ARE/XRE-xan and XRE-AhR elements indicate that both are induced significantly by benzo(α)pyrene, an aryl hydrocarbon widespread in the environment, as well as by xanthotoxin, an allelochemical encountered in their hostplants.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15475306</pmid><doi>10.1016/j.ibmb.2004.06.014</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0965-1748 |
| ispartof | Insect biochemistry and molecular biology, 2004-10, Vol.34 (10), p.1129-1139 |
| issn | 0965-1748 1879-0240 |
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| subjects | allelochemicals Animals Aryl Hydrocarbon Hydroxylases - genetics Aryl hydrocarbon responses Base Sequence Benzo(a)pyrene - pharmacology benzopyrene butterflies Butterflies - drug effects Butterflies - enzymology Butterflies - genetics Conserved Sequence conserved sequences coumarins CYP6B1 promoter CYP6B4 promoter cytochrome P-450 Cytochrome P450s deletion mutants DNA - genetics Furanocoumarin responses Furocoumarins - pharmacology gene expression gene induction Genes, Insect - drug effects Insect–plant interactions metabolic detoxification methoxsalen Methoxsalen - pharmacology Molecular Sequence Data mutants Mutation Papilio Papilio glaucus Papilionidae promoter regions Promoter Regions, Genetic - drug effects psoralens reporter genes response elements Sequence Deletion Sequence Homology, Nucleic Acid transcription (genetics) Transcription, Genetic - drug effects Transcriptional regulation Transfection |
| title | Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription |
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